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Molecularly imprinted polymer as SPE sorbent for selective extraction of melamine in dairy products.
Talanta. 2009 Dec 15; 80(2):821-5.T

Abstract

In this paper, a highly selective sample cleanup procedure combining molecular imprinting and solid-phase extraction (MI-SPE) was developed for the isolation of melamine in dairy products. The molecularly imprinted polymer (MIP) was prepared using melamine as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linking monomer. The melamine imprinted polymer was used as selective sorbent for the solid-phase extraction of melamine from dairy products. An off-line MI-SPE method followed by high-performance liquid chromatography with diode-array detection for the detection of melamine was also established. The mean recoveries of melamine from ultra-heat treatment (UHT) milk and milk powders were 92.9-98.0% and 91.6-102.8%, respectively. Good linearity was obtained from 0.5 microM to 10 microM (r>0.999) with a quantitation limit of 0.5 micromol/L (0.06 ppm) which was sufficient to analyse melamine at the maximum level permitted by U.S. Food and Drug Administration (1 ppm) in dairy products. It was demonstrated that the proposed MI-SPE-HPLC method could be applied to direct determination of melamine in dairy products.

Authors+Show Affiliations

The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, PR China. hhyang@fio.org.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19836558

Citation

Yang, Huang-Hao, et al. "Molecularly Imprinted Polymer as SPE Sorbent for Selective Extraction of Melamine in Dairy Products." Talanta, vol. 80, no. 2, 2009, pp. 821-5.
Yang HH, Zhou WH, Guo XC, et al. Molecularly imprinted polymer as SPE sorbent for selective extraction of melamine in dairy products. Talanta. 2009;80(2):821-5.
Yang, H. H., Zhou, W. H., Guo, X. C., Chen, F. R., Zhao, H. Q., Lin, L. M., & Wang, X. R. (2009). Molecularly imprinted polymer as SPE sorbent for selective extraction of melamine in dairy products. Talanta, 80(2), 821-5. https://doi.org/10.1016/j.talanta.2009.07.067
Yang HH, et al. Molecularly Imprinted Polymer as SPE Sorbent for Selective Extraction of Melamine in Dairy Products. Talanta. 2009 Dec 15;80(2):821-5. PubMed PMID: 19836558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly imprinted polymer as SPE sorbent for selective extraction of melamine in dairy products. AU - Yang,Huang-Hao, AU - Zhou,Wen-Hui, AU - Guo,Xiu-Chun, AU - Chen,Fa-Rong, AU - Zhao,Heng-Qiang, AU - Lin,Li-Ming, AU - Wang,Xiao-Ru, Y1 - 2009/08/08/ PY - 2009/05/19/received PY - 2009/07/28/revised PY - 2009/07/31/accepted PY - 2009/10/20/entrez PY - 2009/10/20/pubmed PY - 2010/1/21/medline SP - 821 EP - 5 JF - Talanta JO - Talanta VL - 80 IS - 2 N2 - In this paper, a highly selective sample cleanup procedure combining molecular imprinting and solid-phase extraction (MI-SPE) was developed for the isolation of melamine in dairy products. The molecularly imprinted polymer (MIP) was prepared using melamine as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linking monomer. The melamine imprinted polymer was used as selective sorbent for the solid-phase extraction of melamine from dairy products. An off-line MI-SPE method followed by high-performance liquid chromatography with diode-array detection for the detection of melamine was also established. The mean recoveries of melamine from ultra-heat treatment (UHT) milk and milk powders were 92.9-98.0% and 91.6-102.8%, respectively. Good linearity was obtained from 0.5 microM to 10 microM (r>0.999) with a quantitation limit of 0.5 micromol/L (0.06 ppm) which was sufficient to analyse melamine at the maximum level permitted by U.S. Food and Drug Administration (1 ppm) in dairy products. It was demonstrated that the proposed MI-SPE-HPLC method could be applied to direct determination of melamine in dairy products. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19836558/Molecularly_imprinted_polymer_as_SPE_sorbent_for_selective_extraction_of_melamine_in_dairy_products_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(09)00645-6 DB - PRIME DP - Unbound Medicine ER -