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Amperometric detection of dopamine based on tyrosinase-SWNTs-Ppy composite electrode.
Talanta. 2009 Dec 15; 80(2):1007-11.T

Abstract

A novel 3-dimensional single wall carbon nanotubes (SWNTs)-polypyrrole (Ppy) composite was prepared as an electrode by chemically polymerizing polypyrrole onto SWNTs using a LiClO(4) oxidant. This composite electrode was characterized by scanning electron microscopy (SEM) and cyclic voltammetry with 1 mM [Fe(CN)(6)](-3)/[Fe(CN)(6)](-4). The SWNTs were thickly coated with chemically polymerized polypyrrole and the composite had many surface pores and crevices which could enhance mass transfer. The SWNTs-Ppy composite electrode showed a large specific surface area (30 m(2)/g) and a good reproducible current response, at about 100 times the peak current of a glassy carbon electrode (GCE). The diffusion coefficient was calculated to be 4.81x10(-6) cm(2)/s. As a biosensor application, tyrosinase was immobilized on the functionalized SWNTs and tyrosinase-SWNTs-Ppy composite was prepared in the same manner. This tyrosinase-SWNT-Ppy composite electrode was used for amperometric detection of dopamine in the presence of ascorbic acid and showed high sensitivity (467 mA/M cm(2)) and lower detection limit (5 microM) compared to previous reports.

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Authors+Show Affiliations

Min K
School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
Yoo YJ
No affiliation info available

MeSH

Ascorbic AcidBiosensing TechniquesDopamineElectrochemical TechniquesElectrodesEnzymes, ImmobilizedKineticsMicroscopy, Electron, ScanningMonophenol MonooxygenaseNanotubes, CarbonPolymersPyrroles

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19836587

Citation

Min, Kyoungseon, and Young Je Yoo. "Amperometric Detection of Dopamine Based On tyrosinase-SWNTs-Ppy Composite Electrode." Talanta, vol. 80, no. 2, 2009, pp. 1007-11.
Min K, Yoo YJ. Amperometric detection of dopamine based on tyrosinase-SWNTs-Ppy composite electrode. Talanta. 2009;80(2):1007-11.
Min, K., & Yoo, Y. J. (2009). Amperometric detection of dopamine based on tyrosinase-SWNTs-Ppy composite electrode. Talanta, 80(2), 1007-11. https://doi.org/10.1016/j.talanta.2009.08.032
Min K, Yoo YJ. Amperometric Detection of Dopamine Based On tyrosinase-SWNTs-Ppy Composite Electrode. Talanta. 2009 Dec 15;80(2):1007-11. PubMed PMID: 19836587.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amperometric detection of dopamine based on tyrosinase-SWNTs-Ppy composite electrode. AU - Min,Kyoungseon, AU - Yoo,Young Je, Y1 - 2009/08/29/ PY - 2009/07/31/received PY - 2009/08/24/accepted PY - 2009/10/20/entrez PY - 2009/10/20/pubmed PY - 2010/1/21/medline SP - 1007 EP - 11 JF - Talanta JO - Talanta VL - 80 IS - 2 N2 - A novel 3-dimensional single wall carbon nanotubes (SWNTs)-polypyrrole (Ppy) composite was prepared as an electrode by chemically polymerizing polypyrrole onto SWNTs using a LiClO(4) oxidant. This composite electrode was characterized by scanning electron microscopy (SEM) and cyclic voltammetry with 1 mM [Fe(CN)(6)](-3)/[Fe(CN)(6)](-4). The SWNTs were thickly coated with chemically polymerized polypyrrole and the composite had many surface pores and crevices which could enhance mass transfer. The SWNTs-Ppy composite electrode showed a large specific surface area (30 m(2)/g) and a good reproducible current response, at about 100 times the peak current of a glassy carbon electrode (GCE). The diffusion coefficient was calculated to be 4.81x10(-6) cm(2)/s. As a biosensor application, tyrosinase was immobilized on the functionalized SWNTs and tyrosinase-SWNTs-Ppy composite was prepared in the same manner. This tyrosinase-SWNT-Ppy composite electrode was used for amperometric detection of dopamine in the presence of ascorbic acid and showed high sensitivity (467 mA/M cm(2)) and lower detection limit (5 microM) compared to previous reports. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19836587/Amperometric_detection_of_dopamine_based_on_tyrosinase_SWNTs_Ppy_composite_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(09)00677-8 DB - PRIME DP - Unbound Medicine ER -