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Insect bite hypersensitivity in the horse: comparison of IgE-binding proteins in salivary gland extracts from Simulium vittatum and Culicoides nubeculosus.
Vet Immunol Immunopathol. 2009 Nov 15; 132(1):62-7.VI

Abstract

Insect bite hypersensitivity (IBH) is an IgE-mediated allergic dermatitis of horses caused by bites of insects such as Culicoides or Simulium spp. The aim of the present study was to compare the IgE-binding pattern of sera of IBH-affected horses to Culicoides nubeculosus and Simulium vittatum salivary gland extracts (SGE). Individual IgE responses to proteins of S. vittatum and C. nubeculosus SGEs were evaluated in 15 IBH-affected and three healthy horses on immunoblots. Fourteen out of the 15 IBH-affected but none of the healthy horses showed individual IgE binding patterns to seven and six main protein bands in C. nubeculosus and S. vittatum SGE, respectively. These 14 sera showed IgE-binding to proteins from SGE of both C. nubeculosus and S. vittatum, but they reacted with fewer protein bands derived from S. vittatum than from C. nubeculosus SGE. Sera showing IgE-binding to a 32 kDa band from C. nubeculosus always bound to a 32 kDa band from S. vittatum. Similarly, all sera binding to a 70 kDa band from C. nubeculosus reacted with a corresponding band in S. vittatum SGE. The 70 kDa bands from S. vittatum and C. nubeculosus were identified by mass spectrometry as heat shock protein-70-cognate-3.

Authors+Show Affiliations

Department of Clinical Research-VPH, Vetsuisse Faculty, University of Berne, Berne, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19836841

Citation

Hellberg, W, et al. "Insect Bite Hypersensitivity in the Horse: Comparison of IgE-binding Proteins in Salivary Gland Extracts From Simulium Vittatum and Culicoides Nubeculosus." Veterinary Immunology and Immunopathology, vol. 132, no. 1, 2009, pp. 62-7.
Hellberg W, Mellor PS, Torsteinsdóttir S, et al. Insect bite hypersensitivity in the horse: comparison of IgE-binding proteins in salivary gland extracts from Simulium vittatum and Culicoides nubeculosus. Vet Immunol Immunopathol. 2009;132(1):62-7.
Hellberg, W., Mellor, P. S., Torsteinsdóttir, S., & Marti, E. (2009). Insect bite hypersensitivity in the horse: comparison of IgE-binding proteins in salivary gland extracts from Simulium vittatum and Culicoides nubeculosus. Veterinary Immunology and Immunopathology, 132(1), 62-7. https://doi.org/10.1016/j.vetimm.2009.09.016
Hellberg W, et al. Insect Bite Hypersensitivity in the Horse: Comparison of IgE-binding Proteins in Salivary Gland Extracts From Simulium Vittatum and Culicoides Nubeculosus. Vet Immunol Immunopathol. 2009 Nov 15;132(1):62-7. PubMed PMID: 19836841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insect bite hypersensitivity in the horse: comparison of IgE-binding proteins in salivary gland extracts from Simulium vittatum and Culicoides nubeculosus. AU - Hellberg,W, AU - Mellor,P S, AU - Torsteinsdóttir,S, AU - Marti,E, Y1 - 2009/09/24/ PY - 2009/10/20/entrez PY - 2009/10/20/pubmed PY - 2010/1/12/medline SP - 62 EP - 7 JF - Veterinary immunology and immunopathology JO - Vet Immunol Immunopathol VL - 132 IS - 1 N2 - Insect bite hypersensitivity (IBH) is an IgE-mediated allergic dermatitis of horses caused by bites of insects such as Culicoides or Simulium spp. The aim of the present study was to compare the IgE-binding pattern of sera of IBH-affected horses to Culicoides nubeculosus and Simulium vittatum salivary gland extracts (SGE). Individual IgE responses to proteins of S. vittatum and C. nubeculosus SGEs were evaluated in 15 IBH-affected and three healthy horses on immunoblots. Fourteen out of the 15 IBH-affected but none of the healthy horses showed individual IgE binding patterns to seven and six main protein bands in C. nubeculosus and S. vittatum SGE, respectively. These 14 sera showed IgE-binding to proteins from SGE of both C. nubeculosus and S. vittatum, but they reacted with fewer protein bands derived from S. vittatum than from C. nubeculosus SGE. Sera showing IgE-binding to a 32 kDa band from C. nubeculosus always bound to a 32 kDa band from S. vittatum. Similarly, all sera binding to a 70 kDa band from C. nubeculosus reacted with a corresponding band in S. vittatum SGE. The 70 kDa bands from S. vittatum and C. nubeculosus were identified by mass spectrometry as heat shock protein-70-cognate-3. SN - 1873-2534 UR - https://www.unboundmedicine.com/medline/citation/19836841/Insect_bite_hypersensitivity_in_the_horse:_comparison_of_IgE_binding_proteins_in_salivary_gland_extracts_from_Simulium_vittatum_and_Culicoides_nubeculosus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2427(09)00319-5 DB - PRIME DP - Unbound Medicine ER -