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Characterization of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus spp. isolated from US West Coast public marine beaches.
J Antimicrob Chemother. 2009 Dec; 64(6):1148-55.JA

Abstract

OBJECTIVES

The aim of this study was to isolate and characterize methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative Staphylococcus spp. (MRCoNS) from marine water and intertidal beach sand from public beaches in Washington State, USA.

METHODS

Fifty-one staphylococci from Washington State beaches were characterized using antimicrobial susceptibility testing, carriage of acquired tetracycline and/or macrolide resistance genes, staphylococcal cassette chromosome mec (SCCmec) typing, the BBL Crystal Gram-Positive ID System and/or 16S rRNA sequencing, coagulase test and multilocus sequence typing (MLST) for MRSA.

RESULTS

Five multidrug-resistant MRSA SCCmec type I, of which three were MLST type ST45, one ST59 and one a new MLST type, ST1405, plus one susceptible non-typeable (NT) MRSA ST30 were characterized. Thirty-three MRCoNS isolates, representing 21 strains from 9 Staphylococcus spp., carried a range of SCCmec types [I (2), II (6), III (3), V (2), I/II (1) and NT (7)] and varied in their antibiotic susceptibility to other antibiotic classes and carriage of acquired tetracycline/macrolide resistance gene(s). MRSA and MRCoNS donors co-transferred tet(M) and erm(A) genes to an Enterococcus faecalis recipient at a frequency of 10(-8).

CONCLUSIONS

This is the first report of MRSA and MRCoNS isolated from marine water and intertidal beach sand. The MLST types and antibiotic carriage of five MRSA isolates were similar to hospital MRSA isolates rather than US community-acquired MRSA isolates. Our results suggest that public marine beaches may be a reservoir for transmission of MRSA to beach visitors as well as an ecosystem for exchange of antibiotic resistance genes among staphylococci and related genera.

Authors+Show Affiliations

Department of Environmental & Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19837712

Citation

Soge, Olusegun O., et al. "Characterization of Methicillin-resistant Staphylococcus Aureus and Methicillin-resistant Coagulase-negative Staphylococcus Spp. Isolated From US West Coast Public Marine Beaches." The Journal of Antimicrobial Chemotherapy, vol. 64, no. 6, 2009, pp. 1148-55.
Soge OO, Meschke JS, No DB, et al. Characterization of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus spp. isolated from US West Coast public marine beaches. J Antimicrob Chemother. 2009;64(6):1148-55.
Soge, O. O., Meschke, J. S., No, D. B., & Roberts, M. C. (2009). Characterization of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus spp. isolated from US West Coast public marine beaches. The Journal of Antimicrobial Chemotherapy, 64(6), 1148-55. https://doi.org/10.1093/jac/dkp368
Soge OO, et al. Characterization of Methicillin-resistant Staphylococcus Aureus and Methicillin-resistant Coagulase-negative Staphylococcus Spp. Isolated From US West Coast Public Marine Beaches. J Antimicrob Chemother. 2009;64(6):1148-55. PubMed PMID: 19837712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus spp. isolated from US West Coast public marine beaches. AU - Soge,Olusegun O, AU - Meschke,John S, AU - No,David B, AU - Roberts,Marilyn C, Y1 - 2009/10/16/ PY - 2009/10/20/entrez PY - 2009/10/20/pubmed PY - 2010/2/13/medline SP - 1148 EP - 55 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 64 IS - 6 N2 - OBJECTIVES: The aim of this study was to isolate and characterize methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative Staphylococcus spp. (MRCoNS) from marine water and intertidal beach sand from public beaches in Washington State, USA. METHODS: Fifty-one staphylococci from Washington State beaches were characterized using antimicrobial susceptibility testing, carriage of acquired tetracycline and/or macrolide resistance genes, staphylococcal cassette chromosome mec (SCCmec) typing, the BBL Crystal Gram-Positive ID System and/or 16S rRNA sequencing, coagulase test and multilocus sequence typing (MLST) for MRSA. RESULTS: Five multidrug-resistant MRSA SCCmec type I, of which three were MLST type ST45, one ST59 and one a new MLST type, ST1405, plus one susceptible non-typeable (NT) MRSA ST30 were characterized. Thirty-three MRCoNS isolates, representing 21 strains from 9 Staphylococcus spp., carried a range of SCCmec types [I (2), II (6), III (3), V (2), I/II (1) and NT (7)] and varied in their antibiotic susceptibility to other antibiotic classes and carriage of acquired tetracycline/macrolide resistance gene(s). MRSA and MRCoNS donors co-transferred tet(M) and erm(A) genes to an Enterococcus faecalis recipient at a frequency of 10(-8). CONCLUSIONS: This is the first report of MRSA and MRCoNS isolated from marine water and intertidal beach sand. The MLST types and antibiotic carriage of five MRSA isolates were similar to hospital MRSA isolates rather than US community-acquired MRSA isolates. Our results suggest that public marine beaches may be a reservoir for transmission of MRSA to beach visitors as well as an ecosystem for exchange of antibiotic resistance genes among staphylococci and related genera. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/19837712/Characterization_of_methicillin_resistant_Staphylococcus_aureus_and_methicillin_resistant_coagulase_negative_Staphylococcus_spp__isolated_from_US_West_Coast_public_marine_beaches_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkp368 DB - PRIME DP - Unbound Medicine ER -