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Determination of paclitaxel and its analogues in the needles of Taxus species by using negative pressure cavitation extraction followed by HPLC-MS-MS.
J Sep Sci. 2009 Nov; 32(22):3958-66.JS

Abstract

A new separation method, negative pressure cavitation (NPC) extraction followed by HPLC-MS-MS for the determination of paclitaxel and its analogues in the needles of Taxus species is described in this study. Compared with three conventional extraction methods, NPC is a more effective, economical, and facile method for the separation of nature compounds from herbal plants. In contrast to high-temperature extraction, NPC at low temperature can minimize undesirable reactions such as thermal decomposition or degradation. In addition, the extraction mechanism of NPC was also illuminated. The effects of vacuum degree, extraction solvent, ratio of solid to liquid, time, and times extraction were studied and optimized. The optimum extraction conditions were as follows: vacuum degree -0.03 MPa, solvent 80% v/v alcohol, ratio of material to liquid 1:15 (g/mL), extraction time 60 min, extraction (x3). Using these conditions, the recoveries of 10-deacetyl-7-xylosylpaclitaxel, 10-deacetylpaclitaxel, cephalomannine, and paclitaxel were higher than 95.88, 95.82, 94.85, and 96.18%, respectively. The contents of paclitaxel, 10-deacetyl-7-xylosylpaclitaxel, 10-deacetylpaclitaxel, and cephalomannine for Taxus chinensis were 0.0053, 0.0467, 0.0132, and 0.0076%, and 0.0067, 0.0153, 0.0047, and 0.0064% for Taxus cuspidata, respectively. Furthermore, a comparison of SEM images observed the morphological changes of microstructures and cellular damage in yew needles.

Authors+Show Affiliations

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

19839015

Citation

Li, Shuangming, et al. "Determination of Paclitaxel and Its Analogues in the Needles of Taxus Species By Using Negative Pressure Cavitation Extraction Followed By HPLC-MS-MS." Journal of Separation Science, vol. 32, no. 22, 2009, pp. 3958-66.
Li S, Fu Y, Zu Y, et al. Determination of paclitaxel and its analogues in the needles of Taxus species by using negative pressure cavitation extraction followed by HPLC-MS-MS. J Sep Sci. 2009;32(22):3958-66.
Li, S., Fu, Y., Zu, Y., Zu, B., Wang, Y., & Efferth, T. (2009). Determination of paclitaxel and its analogues in the needles of Taxus species by using negative pressure cavitation extraction followed by HPLC-MS-MS. Journal of Separation Science, 32(22), 3958-66. https://doi.org/10.1002/jssc.200900402
Li S, et al. Determination of Paclitaxel and Its Analogues in the Needles of Taxus Species By Using Negative Pressure Cavitation Extraction Followed By HPLC-MS-MS. J Sep Sci. 2009;32(22):3958-66. PubMed PMID: 19839015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of paclitaxel and its analogues in the needles of Taxus species by using negative pressure cavitation extraction followed by HPLC-MS-MS. AU - Li,Shuangming, AU - Fu,Yujie, AU - Zu,Yuangang, AU - Zu,Baishi, AU - Wang,Ying, AU - Efferth,Thomas, PY - 2009/10/20/entrez PY - 2009/10/20/pubmed PY - 2010/3/10/medline SP - 3958 EP - 66 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 22 N2 - A new separation method, negative pressure cavitation (NPC) extraction followed by HPLC-MS-MS for the determination of paclitaxel and its analogues in the needles of Taxus species is described in this study. Compared with three conventional extraction methods, NPC is a more effective, economical, and facile method for the separation of nature compounds from herbal plants. In contrast to high-temperature extraction, NPC at low temperature can minimize undesirable reactions such as thermal decomposition or degradation. In addition, the extraction mechanism of NPC was also illuminated. The effects of vacuum degree, extraction solvent, ratio of solid to liquid, time, and times extraction were studied and optimized. The optimum extraction conditions were as follows: vacuum degree -0.03 MPa, solvent 80% v/v alcohol, ratio of material to liquid 1:15 (g/mL), extraction time 60 min, extraction (x3). Using these conditions, the recoveries of 10-deacetyl-7-xylosylpaclitaxel, 10-deacetylpaclitaxel, cephalomannine, and paclitaxel were higher than 95.88, 95.82, 94.85, and 96.18%, respectively. The contents of paclitaxel, 10-deacetyl-7-xylosylpaclitaxel, 10-deacetylpaclitaxel, and cephalomannine for Taxus chinensis were 0.0053, 0.0467, 0.0132, and 0.0076%, and 0.0067, 0.0153, 0.0047, and 0.0064% for Taxus cuspidata, respectively. Furthermore, a comparison of SEM images observed the morphological changes of microstructures and cellular damage in yew needles. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/19839015/Determination_of_paclitaxel_and_its_analogues_in_the_needles_of_Taxus_species_by_using_negative_pressure_cavitation_extraction_followed_by_HPLC_MS_MS_ L2 - https://doi.org/10.1002/jssc.200900402 DB - PRIME DP - Unbound Medicine ER -