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Induction of germline apoptosis by cobalt and relevant signal transduction pathways in Caenorhabditis elegans.
Toxicol Mech Methods. 2009 Nov; 19(9):541-6.TM

Abstract

Many investigations showed that cobalt exposure could induce apoptosis both in cells and tissues. However, appropriate in vivo animal models to assess the underlying mechanisms of cobalt-induced apoptosis are currently unavailable. The model organism, Caenorhabditis elegans, has been shown to be a good model for evaluating many biological processes. This study detected significant cobalt induced germline cell apoptosis after 12-h exposure; thus demonstrating that C. elegans could be a mammalian in vivo substitute model to study mechanisms of apoptosis. Then knockout gene C. elegans strains were utilized to investigate the relationship between cobalt-induced apoptosis and relevant signal pathways, which were involved in DNA damage and repair, apoptosis regulation, and damage signal transduction. The results presented here demonstrated that cobalt-induced apoptosis was independent of the DNA damage response gene, such as hus-1, p53/cep-1, and egl-1. The loss-of-function of the genes that related to JNK and p38 MAPK signaling cascades suppressed cobalt-induced germline apoptosis, while ERK signaling cascades have no effect on the cobalt-induced germline apoptosis.

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Authors+Show Affiliations

Chong R
Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei, Anhui 230031, PR China. r.chong1981@163.com
Ke-zhou C
No affiliation info available
Zeng-liang Y
No affiliation info available

MeSH

AnimalsApoptosisCaenorhabditis elegansCobaltDNA DamageDNA RepairGene Expression RegulationGene Knockout TechniquesGerm CellsMAP Kinase Kinase 4Mitogen-Activated Protein KinasesModels, AnimalMutagensNickelSignal TransductionToxicity Testsp38 Mitogen-Activated Protein Kinases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19839723

Citation

Chong, Ren, et al. "Induction of Germline Apoptosis By Cobalt and Relevant Signal Transduction Pathways in Caenorhabditis Elegans." Toxicology Mechanisms and Methods, vol. 19, no. 9, 2009, pp. 541-6.
Chong R, Ke-zhou C, Zeng-liang Y. Induction of germline apoptosis by cobalt and relevant signal transduction pathways in Caenorhabditis elegans. Toxicol Mech Methods. 2009;19(9):541-6.
Chong, R., Ke-zhou, C., & Zeng-liang, Y. (2009). Induction of germline apoptosis by cobalt and relevant signal transduction pathways in Caenorhabditis elegans. Toxicology Mechanisms and Methods, 19(9), 541-6. https://doi.org/10.3109/15376510903350363
Chong R, Ke-zhou C, Zeng-liang Y. Induction of Germline Apoptosis By Cobalt and Relevant Signal Transduction Pathways in Caenorhabditis Elegans. Toxicol Mech Methods. 2009;19(9):541-6. PubMed PMID: 19839723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of germline apoptosis by cobalt and relevant signal transduction pathways in Caenorhabditis elegans. AU - Chong,Ren, AU - Ke-zhou,Cai, AU - Zeng-liang,Yu, PY - 2009/10/21/entrez PY - 2009/10/21/pubmed PY - 2010/1/23/medline SP - 541 EP - 6 JF - Toxicology mechanisms and methods JO - Toxicol Mech Methods VL - 19 IS - 9 N2 - Many investigations showed that cobalt exposure could induce apoptosis both in cells and tissues. However, appropriate in vivo animal models to assess the underlying mechanisms of cobalt-induced apoptosis are currently unavailable. The model organism, Caenorhabditis elegans, has been shown to be a good model for evaluating many biological processes. This study detected significant cobalt induced germline cell apoptosis after 12-h exposure; thus demonstrating that C. elegans could be a mammalian in vivo substitute model to study mechanisms of apoptosis. Then knockout gene C. elegans strains were utilized to investigate the relationship between cobalt-induced apoptosis and relevant signal pathways, which were involved in DNA damage and repair, apoptosis regulation, and damage signal transduction. The results presented here demonstrated that cobalt-induced apoptosis was independent of the DNA damage response gene, such as hus-1, p53/cep-1, and egl-1. The loss-of-function of the genes that related to JNK and p38 MAPK signaling cascades suppressed cobalt-induced germline apoptosis, while ERK signaling cascades have no effect on the cobalt-induced germline apoptosis. SN - 1537-6524 UR - https://www.unboundmedicine.com/medline/citation/19839723/Induction_of_germline_apoptosis_by_cobalt_and_relevant_signal_transduction_pathways_in_Caenorhabditis_elegans_ L2 - https://www.tandfonline.com/doi/full/10.3109/15376510903350363 DB - PRIME DP - Unbound Medicine ER -