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Antioxidant activities of aqueous extract from Agrimonia pilosa Ledeb and its fractions.
Chem Biodivers. 2009 Oct; 6(10):1716-26.CB

Abstract

Agrimonia pilosa Ledeb is used as the tonic for asthenia and fatigue in China. Considering that the energizing effect might be correlated with antioxidant properties, we investigated the antioxidant activities of aqueous extract (AE) from Agrimonia pilosa Ledeb by assessing radical-scavenging and anti-lipid-peroxidation abilities. We found that AE shows a moderate antioxidant activity to scavenge DPPH*, O2(-)* and *OH and inhibit beta-carotene bleaching with IC(50) values of 13.0, 33.2, 351, and 11.9 microg/ml, respectively, while its AcOEt-soluble fraction (ESF) and BuOH soluble fraction (BSF) exhibit remarkable efficiencies. The ESF's IC(50) values of scavenging DPPH*, O2(-)*, and *OH, and inhibiting beta-carotene bleaching are 5.6, 5.8, 171, and 7.6 mircog/ml, respectively, and those of BSF are 7.5, 8.4, 82.0, and 6.2 microg/ml, respectively. In addition, we found that there is a significant correlation between total phenol content and the antioxidant activity determined by O2(-)* and *OH scavenging, and beta-carotene-bleaching assays. Furthermore, HPLC analysis revealed the presence of quercetin, hyperoside, quercitrin, taxifoliol, luteolin-7-O-beta-D-glucopyranoside, and rutin in Agrimonia pilosa Ledeb. Thus, we suggest that the extracts from Agrimonia pilosa Ledeb, could be considered as natural antioxidant sources and dietary nutritional supplements to prevent oxidation-related diseases.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zhu L
Bioengineering College, Chongqing University, Chongqing, 400030, PR China.
Tan J
No affiliation info available
Wang B
No affiliation info available
He R
No affiliation info available
Liu Y
No affiliation info available
Zheng C
No affiliation info available

MeSH

AgrimoniaAntioxidantsChromatography, High Pressure LiquidDietary SupplementsDrugs, Chinese HerbalFree Radical ScavengersGlucosidesInhibitory Concentration 50Lipid PeroxidationLuteolinOxidation-ReductionPhenolsPlant ExtractsQuercetinRutinSolventsWaterbeta Carotene

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19842137

Citation

Zhu, Liancai, et al. "Antioxidant Activities of Aqueous Extract From Agrimonia Pilosa Ledeb and Its Fractions." Chemistry & Biodiversity, vol. 6, no. 10, 2009, pp. 1716-26.
Zhu L, Tan J, Wang B, et al. Antioxidant activities of aqueous extract from Agrimonia pilosa Ledeb and its fractions. Chem Biodivers. 2009;6(10):1716-26.
Zhu, L., Tan, J., Wang, B., He, R., Liu, Y., & Zheng, C. (2009). Antioxidant activities of aqueous extract from Agrimonia pilosa Ledeb and its fractions. Chemistry & Biodiversity, 6(10), 1716-26. https://doi.org/10.1002/cbdv.200800248
Zhu L, et al. Antioxidant Activities of Aqueous Extract From Agrimonia Pilosa Ledeb and Its Fractions. Chem Biodivers. 2009;6(10):1716-26. PubMed PMID: 19842137.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activities of aqueous extract from Agrimonia pilosa Ledeb and its fractions. AU - Zhu,Liancai, AU - Tan,Jun, AU - Wang,Bochu, AU - He,Rui, AU - Liu,Yuping, AU - Zheng,Chao, PY - 2009/10/21/entrez PY - 2009/10/21/pubmed PY - 2010/3/13/medline SP - 1716 EP - 26 JF - Chemistry & biodiversity JO - Chem Biodivers VL - 6 IS - 10 N2 - Agrimonia pilosa Ledeb is used as the tonic for asthenia and fatigue in China. Considering that the energizing effect might be correlated with antioxidant properties, we investigated the antioxidant activities of aqueous extract (AE) from Agrimonia pilosa Ledeb by assessing radical-scavenging and anti-lipid-peroxidation abilities. We found that AE shows a moderate antioxidant activity to scavenge DPPH*, O2(-)* and *OH and inhibit beta-carotene bleaching with IC(50) values of 13.0, 33.2, 351, and 11.9 microg/ml, respectively, while its AcOEt-soluble fraction (ESF) and BuOH soluble fraction (BSF) exhibit remarkable efficiencies. The ESF's IC(50) values of scavenging DPPH*, O2(-)*, and *OH, and inhibiting beta-carotene bleaching are 5.6, 5.8, 171, and 7.6 mircog/ml, respectively, and those of BSF are 7.5, 8.4, 82.0, and 6.2 microg/ml, respectively. In addition, we found that there is a significant correlation between total phenol content and the antioxidant activity determined by O2(-)* and *OH scavenging, and beta-carotene-bleaching assays. Furthermore, HPLC analysis revealed the presence of quercetin, hyperoside, quercitrin, taxifoliol, luteolin-7-O-beta-D-glucopyranoside, and rutin in Agrimonia pilosa Ledeb. Thus, we suggest that the extracts from Agrimonia pilosa Ledeb, could be considered as natural antioxidant sources and dietary nutritional supplements to prevent oxidation-related diseases. SN - 1612-1880 UR - https://www.unboundmedicine.com/medline/citation/19842137/Antioxidant_activities_of_aqueous_extract_from_Agrimonia_pilosa_Ledeb_and_its_fractions_ L2 - https://doi.org/10.1002/cbdv.200800248 DB - PRIME DP - Unbound Medicine ER -