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Inhibitory effect of fucoidan on nitric oxide production in lipopolysaccharide-activated primary microglia.
Clin Exp Pharmacol Physiol. 2010 Apr; 37(4):422-8.CE

Abstract

1. Microglial activation plays an important role in the pathogenesis of neurodegenerative diseases by producing various pro-inflammatory cytokines. Microglia-derived nitric oxide (NO) is critical for the lipopolysaccharide (LPS)-induced selective loss of dopaminergic neurons. 2. Fucoidan is a sulphated polysaccharide extracted from brown seaweeds. It has a variety of biological actions, including anticoagulant, antiviral and anti-inflammatory effects. The aim of the present study was to investigate the effects of fucoidan on LPS-induced cellular activation in microglia and to evaluate the inhibitory mechanisms involved. 3. To investigate the effects of fucoidan on LPS-induced cellular activation in microglia, primary microglial cells were preincubated with fucoidan (31.25, 62.5 and 125 microg/mL) for 10 min, followed by stimulation with LPS (0.01 microg/mL). Then, cell shape and NO production were determined 24 h after LPS stimulation, whereas inducible nitric oxide synthase (iNOS) mRNA and protein expression were determined at 6 and 18 h after LPS stimulation, respectively. To evaluate the inhibitory mechanisms involved, mitogen-activated protein kinase (MAPK) activation was also evaluated. 4. Lipopolysaccharide transformed cells into an amoeboid shape, whereas 62.5 microg/mL fucoidan inhibited this activation. Moreover, 125 microg/mL fucoidan significantly inhibited microglial NO production to 75% of that in LPS-treated group and also significantly diminished the expression of iNOS mRNA and protein by nearly 50%. Fucoidan (125 microg/mL) also suppressed phosphorylation of p38 and extracellular signal-regulated kinase (ERK) by approximately 50%, but not that of c-Jun N-terminal kinase. 5. The results provide the first evidence that fucoidan has a potent inhibitory effect against LPS-induced NO production by microglia. The results also suggest that this inhibitory action of fucoidan involves suppression of p38 and ERK phosphorylation.

Authors+Show Affiliations

Key Laboratory of Neurodegenerative Diseases of Ministry of Education, Capital Medical University, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19843098

Citation

Cui, Yan-Qiu, et al. "Inhibitory Effect of Fucoidan On Nitric Oxide Production in Lipopolysaccharide-activated Primary Microglia." Clinical and Experimental Pharmacology & Physiology, vol. 37, no. 4, 2010, pp. 422-8.
Cui YQ, Zhang LJ, Zhang T, et al. Inhibitory effect of fucoidan on nitric oxide production in lipopolysaccharide-activated primary microglia. Clin Exp Pharmacol Physiol. 2010;37(4):422-8.
Cui, Y. Q., Zhang, L. J., Zhang, T., Luo, D. Z., Jia, Y. J., Guo, Z. X., Zhang, Q. B., Wang, X., & Wang, X. M. (2010). Inhibitory effect of fucoidan on nitric oxide production in lipopolysaccharide-activated primary microglia. Clinical and Experimental Pharmacology & Physiology, 37(4), 422-8. https://doi.org/10.1111/j.1440-1681.2009.05314.x
Cui YQ, et al. Inhibitory Effect of Fucoidan On Nitric Oxide Production in Lipopolysaccharide-activated Primary Microglia. Clin Exp Pharmacol Physiol. 2010;37(4):422-8. PubMed PMID: 19843098.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of fucoidan on nitric oxide production in lipopolysaccharide-activated primary microglia. AU - Cui,Yan-Qiu, AU - Zhang,Li-Juan, AU - Zhang,Ting, AU - Luo,Ding-Zhen, AU - Jia,Yan-Jun, AU - Guo,Zi-Xuan, AU - Zhang,Quan-Bin, AU - Wang,Xuan, AU - Wang,Xiao-Min, Y1 - 2009/10/16/ PY - 2009/10/22/entrez PY - 2009/10/22/pubmed PY - 2010/8/7/medline SP - 422 EP - 8 JF - Clinical and experimental pharmacology & physiology JO - Clin Exp Pharmacol Physiol VL - 37 IS - 4 N2 - 1. Microglial activation plays an important role in the pathogenesis of neurodegenerative diseases by producing various pro-inflammatory cytokines. Microglia-derived nitric oxide (NO) is critical for the lipopolysaccharide (LPS)-induced selective loss of dopaminergic neurons. 2. Fucoidan is a sulphated polysaccharide extracted from brown seaweeds. It has a variety of biological actions, including anticoagulant, antiviral and anti-inflammatory effects. The aim of the present study was to investigate the effects of fucoidan on LPS-induced cellular activation in microglia and to evaluate the inhibitory mechanisms involved. 3. To investigate the effects of fucoidan on LPS-induced cellular activation in microglia, primary microglial cells were preincubated with fucoidan (31.25, 62.5 and 125 microg/mL) for 10 min, followed by stimulation with LPS (0.01 microg/mL). Then, cell shape and NO production were determined 24 h after LPS stimulation, whereas inducible nitric oxide synthase (iNOS) mRNA and protein expression were determined at 6 and 18 h after LPS stimulation, respectively. To evaluate the inhibitory mechanisms involved, mitogen-activated protein kinase (MAPK) activation was also evaluated. 4. Lipopolysaccharide transformed cells into an amoeboid shape, whereas 62.5 microg/mL fucoidan inhibited this activation. Moreover, 125 microg/mL fucoidan significantly inhibited microglial NO production to 75% of that in LPS-treated group and also significantly diminished the expression of iNOS mRNA and protein by nearly 50%. Fucoidan (125 microg/mL) also suppressed phosphorylation of p38 and extracellular signal-regulated kinase (ERK) by approximately 50%, but not that of c-Jun N-terminal kinase. 5. The results provide the first evidence that fucoidan has a potent inhibitory effect against LPS-induced NO production by microglia. The results also suggest that this inhibitory action of fucoidan involves suppression of p38 and ERK phosphorylation. SN - 1440-1681 UR - https://www.unboundmedicine.com/medline/citation/19843098/Inhibitory_effect_of_fucoidan_on_nitric_oxide_production_in_lipopolysaccharide_activated_primary_microglia_ L2 - https://doi.org/10.1111/j.1440-1681.2009.05314.x DB - PRIME DP - Unbound Medicine ER -