TY - JOUR T1 - The role of KATP channels on propofol preconditioning in a cellular model of renal ischemia-reperfusion. AU - Assad,Alexandra R, AU - Delou,João Marcos A, AU - Fonseca,Leonardo M, AU - Villela,Nivaldo R, AU - Nascimento,José Hamilton M, AU - Verçosa,Nubia, AU - Lopes,Anibal Gil, AU - Capella,Márcia A M, PY - 2009/10/22/entrez PY - 2009/10/22/pubmed PY - 2009/11/6/medline SP - 1486 EP - 92 JF - Anesthesia and analgesia JO - Anesth Analg VL - 109 IS - 5 N2 - BACKGROUND: Propofol (2,6-diisopropylphenol) has been shown to protect several organs, including the kidneys, from ischemia-reperfusion (I-R)-induced injury. Although propofol affects adenosine triphosphate-sensitive potassium (K(ATP)) channels in nonrenal tissues, it is still not clear by which mechanisms propofol protects renal cells from such damage. In this study, we investigated whether propofol induces renal preconditioning through renal K(ATP) channels. METHODS: A reversible ATP depletion (antimycin A) followed by restoration of substrate supply in LLC-PK1 cells was used as an in vitro model of renal I-R. Cell viability was assessed by dimethylthiazol-diphenyltetrazol bromide and trypan blue dye exclusion test assays. Apoptosis was evaluated by annexin V-fluorescein isothiocyanate staining by flow cytometry and immunofluorescence. Propofol treatments were initiated at various time intervals: 1 or 24 h before ischemia, only during ischemia, or only during reperfusion. To evaluate the mechanisms of propofol protection, specific K(ATP) channel inhibitors or activators were used in some experiments during propofol pretreatment. RESULTS: Propofol attenuated I-R injury on LLC-PK1 cells when present either 1 or 24 h before initiated I-R, and also during the recovery period, but not when added only during ischemia. Propofol pretreatment significantly protected LLC-PK1 from I-R-induced apoptosis. The protective effect of propofol was prevented by glibenclamide (a sarcolemmal ATP-dependent K(+) channel blocker) and decreased by 5-hydroxidecanoic acid (a mitochondrial ATP-dependent K(+) channel blocker), but it was not modified by diazoxide (a selective opener of ATP-sensitive K(+) channel). CONCLUSION: Propofol protected cells against apoptosis induced by I-R. This protection was probably due to a preconditioning effect of propofol and was, at least in part, mediated by K(ATP) channels. SN - 1526-7598 UR - https://www.unboundmedicine.com/medline/citation/19843786/The_role_of_KATP_channels_on_propofol_preconditioning_in_a_cellular_model_of_renal_ischemia_reperfusion_ DB - PRIME DP - Unbound Medicine ER -