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Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid and high-sensitive detection of Cryptosporidium in water samples.
Water Sci Technol 2009; 60(8):2167-72WS

Abstract

We describe a novel assay for simple, rapid and high-sensitive detection of Cryptosporidium oocysts in water samples using a reverse transcription-loop-mediated isothermal amplification (RT-LAMP). The assay is based on the detection of 18S rRNA specific for Cryptosporidium oocysts. The detection limit of the developed RT-LAMP assay was as low as 6 x 10(-3) oocysts/test tube, which theoretically enables us to detect a Cryptosporidium oocyst and perform duplicated tests even if water samples contain only one oocyst. The developed RT-LAMP assay could more sensitively detect Cryptosporidium oocysts in real water samples than the conventional assay based on microscopic observation.

Authors+Show Affiliations

Department of Environmental Health and Toxicology, Tokyo Metropolitan Institute of Public Health, Shinjuku, Tokyo, Japan. Akiko_Inomata@member.metro.tokyo.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19844064

Citation

Inomata, A, et al. "Development and Evaluation of a Reverse Transcription-loop-mediated Isothermal Amplification Assay for Rapid and High-sensitive Detection of Cryptosporidium in Water Samples." Water Science and Technology : a Journal of the International Association On Water Pollution Research, vol. 60, no. 8, 2009, pp. 2167-72.
Inomata A, Kishida N, Momoda T, et al. Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid and high-sensitive detection of Cryptosporidium in water samples. Water Sci Technol. 2009;60(8):2167-72.
Inomata, A., Kishida, N., Momoda, T., Akiba, M., Izumiyama, S., Yagita, K., & Endo, T. (2009). Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid and high-sensitive detection of Cryptosporidium in water samples. Water Science and Technology : a Journal of the International Association On Water Pollution Research, 60(8), pp. 2167-72. doi:10.2166/wst.2009.599.
Inomata A, et al. Development and Evaluation of a Reverse Transcription-loop-mediated Isothermal Amplification Assay for Rapid and High-sensitive Detection of Cryptosporidium in Water Samples. Water Sci Technol. 2009;60(8):2167-72. PubMed PMID: 19844064.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid and high-sensitive detection of Cryptosporidium in water samples. AU - Inomata,A, AU - Kishida,N, AU - Momoda,T, AU - Akiba,M, AU - Izumiyama,S, AU - Yagita,K, AU - Endo,T, PY - 2009/10/22/entrez PY - 2009/10/22/pubmed PY - 2009/12/18/medline SP - 2167 EP - 72 JF - Water science and technology : a journal of the International Association on Water Pollution Research JO - Water Sci. Technol. VL - 60 IS - 8 N2 - We describe a novel assay for simple, rapid and high-sensitive detection of Cryptosporidium oocysts in water samples using a reverse transcription-loop-mediated isothermal amplification (RT-LAMP). The assay is based on the detection of 18S rRNA specific for Cryptosporidium oocysts. The detection limit of the developed RT-LAMP assay was as low as 6 x 10(-3) oocysts/test tube, which theoretically enables us to detect a Cryptosporidium oocyst and perform duplicated tests even if water samples contain only one oocyst. The developed RT-LAMP assay could more sensitively detect Cryptosporidium oocysts in real water samples than the conventional assay based on microscopic observation. SN - 0273-1223 UR - https://www.unboundmedicine.com/medline/citation/19844064/Development_and_evaluation_of_a_reverse_transcription_loop_mediated_isothermal_amplification_assay_for_rapid_and_high_sensitive_detection_of_Cryptosporidium_in_water_samples_ L2 - https://iwaponline.com/wst/article-lookup/doi/10.2166/wst.2009.599 DB - PRIME DP - Unbound Medicine ER -