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Optimised separation procedures for the simultaneous assay of three plant hormones in liquid biofertilisers.
Phytochem Anal. 2010 Mar-Apr; 21(2):157-62.PA

Abstract

INTRODUCTION

The overuse of petrochemical-based synthetic fertilisers has caused detrimental effects to soil, water supplies, foods and animal health. This, in addition to increased awareness of organic farming, has generated considerable interest in the evaluation of renewable biofertilisers.

OBJECTIVE

The three objectives of the current research were: (1) to evaluate and optimise a solid phase extraction procedure for extraction of three plant hormones, IAA, GA(3) and ABA from two model biofertilisers produced from coconut shells and pineapple peels; (2) to develop an HPLC analysis procedure for the simultaneous separation and quantification of three plant hormones (IAA, GA(3) and ABA); and (3) to evaluate the changes in three plant hormones levels at four different fermentation time periods and varying number of general bacteria, lactic acid bacteria and yeast.

RESULT

An optimised procedure for sample preparation, separation and simultaneous analysis of three plant hormones [indole-3-acetic acid (IAA), gibberellic acid (GA(3)) and abscisic acid (ABA)] produced in liquid biofertilisers was developed. This method involves sample cleanup using a Sep-pack OasisMAX cartridge containing mixed-mode anion-exchange and reverse-phase sorbents that provided optimum recovery of 85.6, 91.9 and 94.3%, respectively, for the three hormones, IAA, GA(3), and ABA. Baseline separation of three hormones was achieved using mobile phase consisting of 1% acetic acid and acetonitrile (75:25, v/v) at pH 4.0. The amounts of hormones produced in liquid biofertilisers were influenced by fruit types, fermentation time and total number of general bacteria, lactic acid bacteria and yeasts. The quantities of three plant hormones produced during fermentation correlated well with the total number of microorganisms present in the liquid biofertilisers.

CONCLUSION

A simple and rapid sample preparation procedure followed by RP-HPLC with UV detection was optimised and developed for simultaneous quantification and identification of three plant hormones namely, IAA, GA(3) and ABA in the liquid biofertilisers. This procedure allows quantification of the three plant hormones in their natural states without any prior derivatisation step. The results presented illustrate that the contents of the three plant hormones depended on the type of fruit wastes, fermentation time and the number of microorganisms found in liquid biofertilisers. This method can be extended to determine the quantity of three hormones in other matrices. This assay procedure will aid in the development of liquid biofertilisers, a valuable alternative fertilisers to promote plant growth. This process will help farmers to reduce production cost and pollution problems.

Authors+Show Affiliations

Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19845039

Citation

Tansupo, Panadda, et al. "Optimised Separation Procedures for the Simultaneous Assay of Three Plant Hormones in Liquid Biofertilisers." Phytochemical Analysis : PCA, vol. 21, no. 2, 2010, pp. 157-62.
Tansupo P, Suwannasom P, Luthria DL, et al. Optimised separation procedures for the simultaneous assay of three plant hormones in liquid biofertilisers. Phytochem Anal. 2010;21(2):157-62.
Tansupo, P., Suwannasom, P., Luthria, D. L., Chanthai, S., & Ruangviriyachai, C. (2010). Optimised separation procedures for the simultaneous assay of three plant hormones in liquid biofertilisers. Phytochemical Analysis : PCA, 21(2), 157-62. https://doi.org/10.1002/pca.1172
Tansupo P, et al. Optimised Separation Procedures for the Simultaneous Assay of Three Plant Hormones in Liquid Biofertilisers. Phytochem Anal. 2010 Mar-Apr;21(2):157-62. PubMed PMID: 19845039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimised separation procedures for the simultaneous assay of three plant hormones in liquid biofertilisers. AU - Tansupo,Panadda, AU - Suwannasom,Pirom, AU - Luthria,Devanand L, AU - Chanthai,Saksit, AU - Ruangviriyachai,Chalerm, PY - 2009/10/22/entrez PY - 2009/10/22/pubmed PY - 2010/6/11/medline SP - 157 EP - 62 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 21 IS - 2 N2 - INTRODUCTION: The overuse of petrochemical-based synthetic fertilisers has caused detrimental effects to soil, water supplies, foods and animal health. This, in addition to increased awareness of organic farming, has generated considerable interest in the evaluation of renewable biofertilisers. OBJECTIVE: The three objectives of the current research were: (1) to evaluate and optimise a solid phase extraction procedure for extraction of three plant hormones, IAA, GA(3) and ABA from two model biofertilisers produced from coconut shells and pineapple peels; (2) to develop an HPLC analysis procedure for the simultaneous separation and quantification of three plant hormones (IAA, GA(3) and ABA); and (3) to evaluate the changes in three plant hormones levels at four different fermentation time periods and varying number of general bacteria, lactic acid bacteria and yeast. RESULT: An optimised procedure for sample preparation, separation and simultaneous analysis of three plant hormones [indole-3-acetic acid (IAA), gibberellic acid (GA(3)) and abscisic acid (ABA)] produced in liquid biofertilisers was developed. This method involves sample cleanup using a Sep-pack OasisMAX cartridge containing mixed-mode anion-exchange and reverse-phase sorbents that provided optimum recovery of 85.6, 91.9 and 94.3%, respectively, for the three hormones, IAA, GA(3), and ABA. Baseline separation of three hormones was achieved using mobile phase consisting of 1% acetic acid and acetonitrile (75:25, v/v) at pH 4.0. The amounts of hormones produced in liquid biofertilisers were influenced by fruit types, fermentation time and total number of general bacteria, lactic acid bacteria and yeasts. The quantities of three plant hormones produced during fermentation correlated well with the total number of microorganisms present in the liquid biofertilisers. CONCLUSION: A simple and rapid sample preparation procedure followed by RP-HPLC with UV detection was optimised and developed for simultaneous quantification and identification of three plant hormones namely, IAA, GA(3) and ABA in the liquid biofertilisers. This procedure allows quantification of the three plant hormones in their natural states without any prior derivatisation step. The results presented illustrate that the contents of the three plant hormones depended on the type of fruit wastes, fermentation time and the number of microorganisms found in liquid biofertilisers. This method can be extended to determine the quantity of three hormones in other matrices. This assay procedure will aid in the development of liquid biofertilisers, a valuable alternative fertilisers to promote plant growth. This process will help farmers to reduce production cost and pollution problems. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/19845039/Optimised_separation_procedures_for_the_simultaneous_assay_of_three_plant_hormones_in_liquid_biofertilisers_ L2 - https://doi.org/10.1002/pca.1172 DB - PRIME DP - Unbound Medicine ER -