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Separation and elucidation of anthocyanins in the fruit of mockstrawberry (Duchesnea indica Focke).
Nat Prod Res. 2009; 23(17):1589-98.NP

Abstract

Anthocyanin pigments in the fruit of mockstrawberry (Duchesnea indica Focke), were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified by a C18 Sep-Pak cartridge open-column chromatography. High-performance liquid chromatography (HPLC) with photodiode array detection and mass spectrometry (MS) was applied for the isolation and composition analysis of anthocyanins in the fruit of mockstrawberry and their aglycones from acid hydrolysis. Three anthocyanins were found in the fruit of mockstrawberry and they were identified as cyanidin 3-O-rutinoside (61%), peonidin 3-O-rutinoside (34%), and petunidin 3-O-rutinoside (5%), respectively, by spectroscopic methods (UV-vis and MS). The two major anthocyanins were isolated by preparative HPLC, and their chemical structures were further characterised by Heuro(1) NMR. On the basis of chromatographic data, the total anthocyanin content was 205 mg g(-1) of the fresh fruit of mockstrawberry.

Authors+Show Affiliations

Faculty of Life Science, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, China. qinchg@nwpu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19851924

Citation

Qin, Chuanguang, et al. "Separation and Elucidation of Anthocyanins in the Fruit of Mockstrawberry (Duchesnea Indica Focke)." Natural Product Research, vol. 23, no. 17, 2009, pp. 1589-98.
Qin C, Li Y, Zhang R, et al. Separation and elucidation of anthocyanins in the fruit of mockstrawberry (Duchesnea indica Focke). Nat Prod Res. 2009;23(17):1589-98.
Qin, C., Li, Y., Zhang, R., Niu, W., & Ding, Y. (2009). Separation and elucidation of anthocyanins in the fruit of mockstrawberry (Duchesnea indica Focke). Natural Product Research, 23(17), 1589-98. https://doi.org/10.1080/14786410802496903
Qin C, et al. Separation and Elucidation of Anthocyanins in the Fruit of Mockstrawberry (Duchesnea Indica Focke). Nat Prod Res. 2009;23(17):1589-98. PubMed PMID: 19851924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and elucidation of anthocyanins in the fruit of mockstrawberry (Duchesnea indica Focke). AU - Qin,Chuanguang, AU - Li,Yang, AU - Zhang,Ruijie, AU - Niu,Weining, AU - Ding,Yan, PY - 2009/10/24/entrez PY - 2009/10/24/pubmed PY - 2010/1/14/medline SP - 1589 EP - 98 JF - Natural product research JO - Nat Prod Res VL - 23 IS - 17 N2 - Anthocyanin pigments in the fruit of mockstrawberry (Duchesnea indica Focke), were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified by a C18 Sep-Pak cartridge open-column chromatography. High-performance liquid chromatography (HPLC) with photodiode array detection and mass spectrometry (MS) was applied for the isolation and composition analysis of anthocyanins in the fruit of mockstrawberry and their aglycones from acid hydrolysis. Three anthocyanins were found in the fruit of mockstrawberry and they were identified as cyanidin 3-O-rutinoside (61%), peonidin 3-O-rutinoside (34%), and petunidin 3-O-rutinoside (5%), respectively, by spectroscopic methods (UV-vis and MS). The two major anthocyanins were isolated by preparative HPLC, and their chemical structures were further characterised by Heuro(1) NMR. On the basis of chromatographic data, the total anthocyanin content was 205 mg g(-1) of the fresh fruit of mockstrawberry. SN - 1478-6427 UR - https://www.unboundmedicine.com/medline/citation/19851924/Separation_and_elucidation_of_anthocyanins_in_the_fruit_of_mockstrawberry__Duchesnea_indica_Focke__ L2 - https://www.tandfonline.com/doi/full/10.1080/14786410802496903 DB - PRIME DP - Unbound Medicine ER -