Tags

Type your tag names separated by a space and hit enter

The Zn finger protein Iguana impacts Hedgehog signaling by promoting ciliogenesis.
Dev Biol 2010; 337(1):148-56DB

Abstract

Hedgehog signaling is critical for metazoan development and requires cilia for pathway activity. The gene iguana was discovered in zebrafish as required for Hedgehog signaling, and encodes a novel Zn finger protein. Planarians are flatworms with robust regenerative capacities and utilize epidermal cilia for locomotion. RNA interference of Smed-iguana in the planarian Schmidtea mediterranea caused cilia loss and failure to regenerate new cilia, but did not cause defects similar to those observed in hedgehog(RNAi) animals. Smed-iguana gene expression was also similar in pattern to the expression of multiple other ciliogenesis genes, but was not required for expression of these ciliogenesis genes. iguana-defective zebrafish had too few motile cilia in pronephric ducts and in Kupffer's vesicle. Kupffer's vesicle promotes left-right asymmetry and iguana mutant embryos had left-right asymmetry defects. Finally, human Iguana proteins (dZIP1 and dZIP1L) localize to the basal bodies of primary cilia and, together, are required for primary cilia formation. Our results indicate that a critical and broadly conserved function for Iguana is in ciliogenesis and that this function has come to be required for Hedgehog signaling in vertebrates.

Authors+Show Affiliations

Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19852954

Citation

Glazer, Andrew M., et al. "The Zn Finger Protein Iguana Impacts Hedgehog Signaling By Promoting Ciliogenesis." Developmental Biology, vol. 337, no. 1, 2010, pp. 148-56.
Glazer AM, Wilkinson AW, Backer CB, et al. The Zn finger protein Iguana impacts Hedgehog signaling by promoting ciliogenesis. Dev Biol. 2010;337(1):148-56.
Glazer, A. M., Wilkinson, A. W., Backer, C. B., Lapan, S. W., Gutzman, J. H., Cheeseman, I. M., & Reddien, P. W. (2010). The Zn finger protein Iguana impacts Hedgehog signaling by promoting ciliogenesis. Developmental Biology, 337(1), pp. 148-56. doi:10.1016/j.ydbio.2009.10.025.
Glazer AM, et al. The Zn Finger Protein Iguana Impacts Hedgehog Signaling By Promoting Ciliogenesis. Dev Biol. 2010 Jan 1;337(1):148-56. PubMed PMID: 19852954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Zn finger protein Iguana impacts Hedgehog signaling by promoting ciliogenesis. AU - Glazer,Andrew M, AU - Wilkinson,Alex W, AU - Backer,Chelsea B, AU - Lapan,Sylvain W, AU - Gutzman,Jennifer H, AU - Cheeseman,Iain M, AU - Reddien,Peter W, Y1 - 2009/10/21/ PY - 2009/10/13/received PY - 2009/10/15/accepted PY - 2009/10/27/entrez PY - 2009/10/27/pubmed PY - 2009/12/16/medline SP - 148 EP - 56 JF - Developmental biology JO - Dev. Biol. VL - 337 IS - 1 N2 - Hedgehog signaling is critical for metazoan development and requires cilia for pathway activity. The gene iguana was discovered in zebrafish as required for Hedgehog signaling, and encodes a novel Zn finger protein. Planarians are flatworms with robust regenerative capacities and utilize epidermal cilia for locomotion. RNA interference of Smed-iguana in the planarian Schmidtea mediterranea caused cilia loss and failure to regenerate new cilia, but did not cause defects similar to those observed in hedgehog(RNAi) animals. Smed-iguana gene expression was also similar in pattern to the expression of multiple other ciliogenesis genes, but was not required for expression of these ciliogenesis genes. iguana-defective zebrafish had too few motile cilia in pronephric ducts and in Kupffer's vesicle. Kupffer's vesicle promotes left-right asymmetry and iguana mutant embryos had left-right asymmetry defects. Finally, human Iguana proteins (dZIP1 and dZIP1L) localize to the basal bodies of primary cilia and, together, are required for primary cilia formation. Our results indicate that a critical and broadly conserved function for Iguana is in ciliogenesis and that this function has come to be required for Hedgehog signaling in vertebrates. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/19852954/The_Zn_finger_protein_Iguana_impacts_Hedgehog_signaling_by_promoting_ciliogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(09)01284-6 DB - PRIME DP - Unbound Medicine ER -