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Connexin 43 gene expression in male and female gonads of porcine offspring following in utero exposure to an anti-androgen, flutamide.
Acta Histochem. 2011 Jan; 113(1):6-12.AH

Abstract

The aim of this study was to show the effect of maternal exposure to flutamide on connexin 43 (Cx43) gene expression in testes and ovaries of 2-day-old piglets. Additionally, anogenital distance (AGD) was measured both in male and female offspring. Immunohistochemistry, Western blotting, and RT-PCR were performed to assess the immunoreactivity and the presence of Cx43 protein and it's mRNA, respectively. Following flutamide exposure strong immunostaining for Cx43 was observed between testicular Leydig cells, between granulosa cells of primary follicles, and between interstitial cells surrounding clusters of oocyte nests in the ovarian cortex as in the respective controls. Differences between the flutamide-treated groups and the controls obtained by qualitative immunohistochemistry were confirmed by quantitative image analysis (*P<0.05; **P<0.01). In Western blotting, Cx43 appeared as a band of 43kDa, whereas electrophoresis revealed PCR products of the predicted sizes. Screening for Cx43 expression revealed the presence of a transcript, both in control and in flutamide-treated pigs. The AGD values differed significantly from the control (*P<0.05). Overall, since no obvious changes in gonad morphology were observed and the Cx43 signal was present in all the examined tissues, it seems likely that androgens acting through ARs are not involved in the control of Cx43 gene expression in neonatal pig gonads.

Authors+Show Affiliations

Department of Endocrinology, Institute of Zoology, Jagiellonian University, Krakow, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19853283

Citation

Durlej, Malgorzata, et al. "Connexin 43 Gene Expression in Male and Female Gonads of Porcine Offspring Following in Utero Exposure to an Anti-androgen, Flutamide." Acta Histochemica, vol. 113, no. 1, 2011, pp. 6-12.
Durlej M, Kopera I, Knapczyk-Stwora K, et al. Connexin 43 gene expression in male and female gonads of porcine offspring following in utero exposure to an anti-androgen, flutamide. Acta Histochem. 2011;113(1):6-12.
Durlej, M., Kopera, I., Knapczyk-Stwora, K., Hejmej, A., Duda, M., Koziorowski, M., Slomczynska, M., & Bilinska, B. (2011). Connexin 43 gene expression in male and female gonads of porcine offspring following in utero exposure to an anti-androgen, flutamide. Acta Histochemica, 113(1), 6-12. https://doi.org/10.1016/j.acthis.2009.07.001
Durlej M, et al. Connexin 43 Gene Expression in Male and Female Gonads of Porcine Offspring Following in Utero Exposure to an Anti-androgen, Flutamide. Acta Histochem. 2011;113(1):6-12. PubMed PMID: 19853283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Connexin 43 gene expression in male and female gonads of porcine offspring following in utero exposure to an anti-androgen, flutamide. AU - Durlej,Malgorzata, AU - Kopera,Ilona, AU - Knapczyk-Stwora,Katarzyna, AU - Hejmej,Anna, AU - Duda,Malgorzata, AU - Koziorowski,Marek, AU - Slomczynska,Maria, AU - Bilinska,Barbara, Y1 - 2009/10/22/ PY - 2009/05/17/received PY - 2009/07/05/revised PY - 2009/07/06/accepted PY - 2009/10/27/entrez PY - 2009/10/27/pubmed PY - 2011/3/26/medline SP - 6 EP - 12 JF - Acta histochemica JO - Acta Histochem VL - 113 IS - 1 N2 - The aim of this study was to show the effect of maternal exposure to flutamide on connexin 43 (Cx43) gene expression in testes and ovaries of 2-day-old piglets. Additionally, anogenital distance (AGD) was measured both in male and female offspring. Immunohistochemistry, Western blotting, and RT-PCR were performed to assess the immunoreactivity and the presence of Cx43 protein and it's mRNA, respectively. Following flutamide exposure strong immunostaining for Cx43 was observed between testicular Leydig cells, between granulosa cells of primary follicles, and between interstitial cells surrounding clusters of oocyte nests in the ovarian cortex as in the respective controls. Differences between the flutamide-treated groups and the controls obtained by qualitative immunohistochemistry were confirmed by quantitative image analysis (*P<0.05; **P<0.01). In Western blotting, Cx43 appeared as a band of 43kDa, whereas electrophoresis revealed PCR products of the predicted sizes. Screening for Cx43 expression revealed the presence of a transcript, both in control and in flutamide-treated pigs. The AGD values differed significantly from the control (*P<0.05). Overall, since no obvious changes in gonad morphology were observed and the Cx43 signal was present in all the examined tissues, it seems likely that androgens acting through ARs are not involved in the control of Cx43 gene expression in neonatal pig gonads. SN - 1618-0372 UR - https://www.unboundmedicine.com/medline/citation/19853283/Connexin_43_gene_expression_in_male_and_female_gonads_of_porcine_offspring_following_in_utero_exposure_to_an_anti_androgen_flutamide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0065-1281(09)00073-7 DB - PRIME DP - Unbound Medicine ER -