Tags

Type your tag names separated by a space and hit enter

The C. elegans dosage compensation complex propagates dynamically and independently of X chromosome sequence.
Curr Biol 2009; 19(21):1777-87CB

Abstract

BACKGROUND

The C. elegans dosage compensation complex (DCC) associates with both X chromosomes of XX animals to reduce X-linked transcript levels. Five DCC members are homologous to subunits of the evolutionarily conserved condensin complex, and two noncondensin subunits are required for DCC recruitment to X.

RESULTS

We investigated the molecular mechanism of DCC recruitment and spreading along X by examining gene expression and the binding patterns of DCC subunits in different stages of development, and in strains harboring X;autosome (X;A) fusions. We show that DCC binding is dynamically specified according to gene activity during development and that the mechanism of DCC spreading is independent of X chromosome DNA sequence. Accordingly, in X;A fusion strains, DCC binding propagates from X-linked recruitment sites onto autosomal promoters as a function of distance. Quantitative analysis of spreading suggests that the condensin-like subunits spread from recruitment sites to promoters more readily than subunits involved in initial X targeting.

CONCLUSIONS

A highly conserved chromatin complex is appropriated to accomplish domain-scale transcriptional regulation during C. elegans development. Unlike X recognition, which is specified partly by DNA sequence, spreading is sequence independent and coupled to transcriptional activity. Similarities to the X recognition and spreading strategies used by the Drosophila DCC suggest mechanisms fundamental to chromosome-scale gene regulation.

Authors+Show Affiliations

Department of Biology, Carolina Center for Genome Sciences and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19853451

Citation

Ercan, Sevinç, et al. "The C. Elegans Dosage Compensation Complex Propagates Dynamically and Independently of X Chromosome Sequence." Current Biology : CB, vol. 19, no. 21, 2009, pp. 1777-87.
Ercan S, Dick LL, Lieb JD. The C. elegans dosage compensation complex propagates dynamically and independently of X chromosome sequence. Curr Biol. 2009;19(21):1777-87.
Ercan, S., Dick, L. L., & Lieb, J. D. (2009). The C. elegans dosage compensation complex propagates dynamically and independently of X chromosome sequence. Current Biology : CB, 19(21), pp. 1777-87. doi:10.1016/j.cub.2009.09.047.
Ercan S, Dick LL, Lieb JD. The C. Elegans Dosage Compensation Complex Propagates Dynamically and Independently of X Chromosome Sequence. Curr Biol. 2009 Nov 17;19(21):1777-87. PubMed PMID: 19853451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The C. elegans dosage compensation complex propagates dynamically and independently of X chromosome sequence. AU - Ercan,Sevinç, AU - Dick,Lindsay L, AU - Lieb,Jason D, Y1 - 2009/10/22/ PY - 2009/08/10/received PY - 2009/09/11/revised PY - 2009/09/15/accepted PY - 2009/10/27/entrez PY - 2009/10/27/pubmed PY - 2010/3/10/medline SP - 1777 EP - 87 JF - Current biology : CB JO - Curr. Biol. VL - 19 IS - 21 N2 - BACKGROUND: The C. elegans dosage compensation complex (DCC) associates with both X chromosomes of XX animals to reduce X-linked transcript levels. Five DCC members are homologous to subunits of the evolutionarily conserved condensin complex, and two noncondensin subunits are required for DCC recruitment to X. RESULTS: We investigated the molecular mechanism of DCC recruitment and spreading along X by examining gene expression and the binding patterns of DCC subunits in different stages of development, and in strains harboring X;autosome (X;A) fusions. We show that DCC binding is dynamically specified according to gene activity during development and that the mechanism of DCC spreading is independent of X chromosome DNA sequence. Accordingly, in X;A fusion strains, DCC binding propagates from X-linked recruitment sites onto autosomal promoters as a function of distance. Quantitative analysis of spreading suggests that the condensin-like subunits spread from recruitment sites to promoters more readily than subunits involved in initial X targeting. CONCLUSIONS: A highly conserved chromatin complex is appropriated to accomplish domain-scale transcriptional regulation during C. elegans development. Unlike X recognition, which is specified partly by DNA sequence, spreading is sequence independent and coupled to transcriptional activity. Similarities to the X recognition and spreading strategies used by the Drosophila DCC suggest mechanisms fundamental to chromosome-scale gene regulation. SN - 1879-0445 UR - https://www.unboundmedicine.com/medline/citation/19853451/The_C__elegans_dosage_compensation_complex_propagates_dynamically_and_independently_of_X_chromosome_sequence_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-9822(09)01759-X DB - PRIME DP - Unbound Medicine ER -