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Fast determination of glutathione by capillary electrophoresis with fluorescence detection using beta-cyclodextrin as modifier.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Dec 01; 877(31):4051-4.JC

Abstract

A rapid analytical method was developed for the quantitative determination of glutathione (GSH) using capillary electrophoresis and fluorescence detection. A fluorescence derivatization reagent, naphthalene-2,3-dicarboxaldehyde (NDA) was successfully applied to label GSH. The optimal derivatization reaction was performed with 5.0 mM NDA, 20 mM borate buffer (pH 9.2) with the reaction time of 4 min at room temperature. The capillary electrophoresis analysis of GSH could be achieved in less than 120 s using 10 mM sodium tetraborate (pH 9.2) containing 2.5 mM beta-cyclodextrin (beta-CD) as the running buffer, and the detection limit of 2.5 x 10(-9) M (S/N=3) was obtained. This method was successfully applied to analyze the content of GSH in tobacco BY-2 cells.

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Authors+Show Affiliations

Zhang LY
Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China. lyzhang26@yahoo.com.cn
Sun MX
No affiliation info available

MeSH

BuffersCell LineElectrophoresis, CapillaryFluorescenceGlutathioneLimit of DetectionNaphthalenesTobaccobeta-Cyclodextrins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19858002

Citation

Zhang, Li-Yao, and Meng-Xiang Sun. "Fast Determination of Glutathione By Capillary Electrophoresis With Fluorescence Detection Using Beta-cyclodextrin as Modifier." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 31, 2009, pp. 4051-4.
Zhang LY, Sun MX. Fast determination of glutathione by capillary electrophoresis with fluorescence detection using beta-cyclodextrin as modifier. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877(31):4051-4.
Zhang, L. Y., & Sun, M. X. (2009). Fast determination of glutathione by capillary electrophoresis with fluorescence detection using beta-cyclodextrin as modifier. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877(31), 4051-4. https://doi.org/10.1016/j.jchromb.2009.10.001
Zhang LY, Sun MX. Fast Determination of Glutathione By Capillary Electrophoresis With Fluorescence Detection Using Beta-cyclodextrin as Modifier. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Dec 1;877(31):4051-4. PubMed PMID: 19858002.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast determination of glutathione by capillary electrophoresis with fluorescence detection using beta-cyclodextrin as modifier. AU - Zhang,Li-Yao, AU - Sun,Meng-Xiang, Y1 - 2009/10/08/ PY - 2009/04/01/received PY - 2009/08/14/revised PY - 2009/10/01/accepted PY - 2009/10/28/entrez PY - 2009/10/28/pubmed PY - 2010/3/3/medline SP - 4051 EP - 4 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 877 IS - 31 N2 - A rapid analytical method was developed for the quantitative determination of glutathione (GSH) using capillary electrophoresis and fluorescence detection. A fluorescence derivatization reagent, naphthalene-2,3-dicarboxaldehyde (NDA) was successfully applied to label GSH. The optimal derivatization reaction was performed with 5.0 mM NDA, 20 mM borate buffer (pH 9.2) with the reaction time of 4 min at room temperature. The capillary electrophoresis analysis of GSH could be achieved in less than 120 s using 10 mM sodium tetraborate (pH 9.2) containing 2.5 mM beta-cyclodextrin (beta-CD) as the running buffer, and the detection limit of 2.5 x 10(-9) M (S/N=3) was obtained. This method was successfully applied to analyze the content of GSH in tobacco BY-2 cells. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/19858002/Fast_determination_of_glutathione_by_capillary_electrophoresis_with_fluorescence_detection_using_beta_cyclodextrin_as_modifier_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(09)00704-1 DB - PRIME DP - Unbound Medicine ER -