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Fluorescent proteins for live cell imaging: opportunities, limitations, and challenges.
IUBMB Life. 2009 Nov; 61(11):1029-42.IL

Abstract

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of the glowing probes.

Authors+Show Affiliations

National Oceanography Centre, University of Southampton, Southampton, UK. joerg.wiedenmann@noc.soton.ac.ukNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

19859977

Citation

Wiedenmann, Jörg, et al. "Fluorescent Proteins for Live Cell Imaging: Opportunities, Limitations, and Challenges." IUBMB Life, vol. 61, no. 11, 2009, pp. 1029-42.
Wiedenmann J, Oswald F, Nienhaus GU. Fluorescent proteins for live cell imaging: opportunities, limitations, and challenges. IUBMB Life. 2009;61(11):1029-42.
Wiedenmann, J., Oswald, F., & Nienhaus, G. U. (2009). Fluorescent proteins for live cell imaging: opportunities, limitations, and challenges. IUBMB Life, 61(11), 1029-42. https://doi.org/10.1002/iub.256
Wiedenmann J, Oswald F, Nienhaus GU. Fluorescent Proteins for Live Cell Imaging: Opportunities, Limitations, and Challenges. IUBMB Life. 2009;61(11):1029-42. PubMed PMID: 19859977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluorescent proteins for live cell imaging: opportunities, limitations, and challenges. AU - Wiedenmann,Jörg, AU - Oswald,Franz, AU - Nienhaus,Gerd Ulrich, PY - 2009/10/28/entrez PY - 2009/10/28/pubmed PY - 2010/3/3/medline SP - 1029 EP - 42 JF - IUBMB life JO - IUBMB Life VL - 61 IS - 11 N2 - The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of the glowing probes. SN - 1521-6551 UR - https://www.unboundmedicine.com/medline/citation/19859977/Fluorescent_proteins_for_live_cell_imaging:_opportunities_limitations_and_challenges_ L2 - https://doi.org/10.1002/iub.256 DB - PRIME DP - Unbound Medicine ER -