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Translational regulation by the 3' untranslated region of the dengue type 2 virus genome.
Am J Trop Med Hyg. 2009 Nov; 81(5):817-24.AJ

Abstract

The role of the 3'untranslated region (UTR) of the dengue virus (DENV) genome during viral translation remains to be elucidated. We assessed the contribution of well-defined RNA elements in the 3'UTR of DENV-2 to viral translation using a virus-induced reporting gene system and deoxyribozymes (DRzs) targeting the 3'UTR of the DENV-2 genome. Results show that mRNAs carrying a deletion of repeated conserved sequence (RCS2)-CS2 are translated less efficiently than wild type mRNAs. However, mRNAs with a deletion of CS1-stem loop (SL) are translated more efficiently. Thus, CS1-SL and RCS2-CS2 may have different effects on translational regulation. Additionally, the translation-suppressing effect of CS1-SL or the SL element is further confirmed in DENV-2-infected cells using DRzs. Mutagenesis studies show that, rather than the secondary structure, nucleotides 10663-10677 and 10709-10723 are responsible for translational suppression of SL. Overall, our results demonstrate that sequences and elements within the DENV-2 3'UTR regulate viral translation.

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Publisher Full Text

Authors+Show Affiliations

Wei Y
Beijing Institute of Microbiology and Epidemiology, People's Republic of China.
Qin C
No affiliation info available
Jiang T
No affiliation info available
Li X
No affiliation info available
Zhao H
No affiliation info available
Liu Z
No affiliation info available
Deng Y
No affiliation info available
Liu R
No affiliation info available
Chen S
No affiliation info available
Yu M
No affiliation info available
Qin E
No affiliation info available

MeSH

3' Untranslated RegionsAnimalsCell LineCricetinaeDengue VirusGene Expression Regulation, ViralGenome, ViralProtein BiosynthesisRNA, MessengerRNA, Viral

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19861617

Citation

Wei, Yan, et al. "Translational Regulation By the 3' Untranslated Region of the Dengue Type 2 Virus Genome." The American Journal of Tropical Medicine and Hygiene, vol. 81, no. 5, 2009, pp. 817-24.
Wei Y, Qin C, Jiang T, et al. Translational regulation by the 3' untranslated region of the dengue type 2 virus genome. Am J Trop Med Hyg. 2009;81(5):817-24.
Wei, Y., Qin, C., Jiang, T., Li, X., Zhao, H., Liu, Z., Deng, Y., Liu, R., Chen, S., Yu, M., & Qin, E. (2009). Translational regulation by the 3' untranslated region of the dengue type 2 virus genome. The American Journal of Tropical Medicine and Hygiene, 81(5), 817-24. https://doi.org/10.4269/ajtmh.2009.08-0595
Wei Y, et al. Translational Regulation By the 3' Untranslated Region of the Dengue Type 2 Virus Genome. Am J Trop Med Hyg. 2009;81(5):817-24. PubMed PMID: 19861617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Translational regulation by the 3' untranslated region of the dengue type 2 virus genome. AU - Wei,Yan, AU - Qin,Chengfeng, AU - Jiang,Tao, AU - Li,Xiaofeng, AU - Zhao,Hui, AU - Liu,Zhongyu, AU - Deng,Yongqiang, AU - Liu,Ran, AU - Chen,Shuiping, AU - Yu,Man, AU - Qin,Ede, PY - 2009/10/29/entrez PY - 2009/10/29/pubmed PY - 2009/11/18/medline SP - 817 EP - 24 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 81 IS - 5 N2 - The role of the 3'untranslated region (UTR) of the dengue virus (DENV) genome during viral translation remains to be elucidated. We assessed the contribution of well-defined RNA elements in the 3'UTR of DENV-2 to viral translation using a virus-induced reporting gene system and deoxyribozymes (DRzs) targeting the 3'UTR of the DENV-2 genome. Results show that mRNAs carrying a deletion of repeated conserved sequence (RCS2)-CS2 are translated less efficiently than wild type mRNAs. However, mRNAs with a deletion of CS1-stem loop (SL) are translated more efficiently. Thus, CS1-SL and RCS2-CS2 may have different effects on translational regulation. Additionally, the translation-suppressing effect of CS1-SL or the SL element is further confirmed in DENV-2-infected cells using DRzs. Mutagenesis studies show that, rather than the secondary structure, nucleotides 10663-10677 and 10709-10723 are responsible for translational suppression of SL. Overall, our results demonstrate that sequences and elements within the DENV-2 3'UTR regulate viral translation. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/19861617/Translational_regulation_by_the_3'_untranslated_region_of_the_dengue_type_2_virus_genome_ L2 - https://ajtmh.org/doi/10.4269/ajtmh.2009.08-0595 DB - PRIME DP - Unbound Medicine ER -