Tags

Type your tag names separated by a space and hit enter

Translational regulation by the 3' untranslated region of the dengue type 2 virus genome.
Am J Trop Med Hyg. 2009 Nov; 81(5):817-24.AJ

Abstract

The role of the 3'untranslated region (UTR) of the dengue virus (DENV) genome during viral translation remains to be elucidated. We assessed the contribution of well-defined RNA elements in the 3'UTR of DENV-2 to viral translation using a virus-induced reporting gene system and deoxyribozymes (DRzs) targeting the 3'UTR of the DENV-2 genome. Results show that mRNAs carrying a deletion of repeated conserved sequence (RCS2)-CS2 are translated less efficiently than wild type mRNAs. However, mRNAs with a deletion of CS1-stem loop (SL) are translated more efficiently. Thus, CS1-SL and RCS2-CS2 may have different effects on translational regulation. Additionally, the translation-suppressing effect of CS1-SL or the SL element is further confirmed in DENV-2-infected cells using DRzs. Mutagenesis studies show that, rather than the secondary structure, nucleotides 10663-10677 and 10709-10723 are responsible for translational suppression of SL. Overall, our results demonstrate that sequences and elements within the DENV-2 3'UTR regulate viral translation.

Authors+Show Affiliations

Beijing Institute of Microbiology and Epidemiology, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19861617

Citation

Wei, Yan, et al. "Translational Regulation By the 3' Untranslated Region of the Dengue Type 2 Virus Genome." The American Journal of Tropical Medicine and Hygiene, vol. 81, no. 5, 2009, pp. 817-24.
Wei Y, Qin C, Jiang T, et al. Translational regulation by the 3' untranslated region of the dengue type 2 virus genome. Am J Trop Med Hyg. 2009;81(5):817-24.
Wei, Y., Qin, C., Jiang, T., Li, X., Zhao, H., Liu, Z., Deng, Y., Liu, R., Chen, S., Yu, M., & Qin, E. (2009). Translational regulation by the 3' untranslated region of the dengue type 2 virus genome. The American Journal of Tropical Medicine and Hygiene, 81(5), 817-24. https://doi.org/10.4269/ajtmh.2009.08-0595
Wei Y, et al. Translational Regulation By the 3' Untranslated Region of the Dengue Type 2 Virus Genome. Am J Trop Med Hyg. 2009;81(5):817-24. PubMed PMID: 19861617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Translational regulation by the 3' untranslated region of the dengue type 2 virus genome. AU - Wei,Yan, AU - Qin,Chengfeng, AU - Jiang,Tao, AU - Li,Xiaofeng, AU - Zhao,Hui, AU - Liu,Zhongyu, AU - Deng,Yongqiang, AU - Liu,Ran, AU - Chen,Shuiping, AU - Yu,Man, AU - Qin,Ede, PY - 2009/10/29/entrez PY - 2009/10/29/pubmed PY - 2009/11/18/medline SP - 817 EP - 24 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 81 IS - 5 N2 - The role of the 3'untranslated region (UTR) of the dengue virus (DENV) genome during viral translation remains to be elucidated. We assessed the contribution of well-defined RNA elements in the 3'UTR of DENV-2 to viral translation using a virus-induced reporting gene system and deoxyribozymes (DRzs) targeting the 3'UTR of the DENV-2 genome. Results show that mRNAs carrying a deletion of repeated conserved sequence (RCS2)-CS2 are translated less efficiently than wild type mRNAs. However, mRNAs with a deletion of CS1-stem loop (SL) are translated more efficiently. Thus, CS1-SL and RCS2-CS2 may have different effects on translational regulation. Additionally, the translation-suppressing effect of CS1-SL or the SL element is further confirmed in DENV-2-infected cells using DRzs. Mutagenesis studies show that, rather than the secondary structure, nucleotides 10663-10677 and 10709-10723 are responsible for translational suppression of SL. Overall, our results demonstrate that sequences and elements within the DENV-2 3'UTR regulate viral translation. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/19861617/Translational_regulation_by_the_3'_untranslated_region_of_the_dengue_type_2_virus_genome_ L2 - https://ajtmh.org/doi/10.4269/ajtmh.2009.08-0595 DB - PRIME DP - Unbound Medicine ER -