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Prunella vulgaris extract and rosmarinic acid prevent UVB-induced DNA damage and oxidative stress in HaCaT keratinocytes.
Arch Dermatol Res. 2010 Apr; 302(3):171-81.AD

Abstract

Solar radiation is a very important exogenous factor in skin pathogenesis and can lead to the development of a number of skin disorders. UVB irradiation is known to induce oxidative stress, inflammation and especially DNA lesions in exposed cells. It is important, therefore, to identify agents that can offer protection against UVB-caused skin damage. Natural compounds have been studied for their possible ability to control/modulate various lifestyle-related diseases. The application of plant compounds/extracts with screening, antioxidant and anti-inflammatory activities may also successfully protect the skin against UV-caused injury. We assessed the potency of Prunella vulgaris extract (PVE) and its main phenolic acid component, rosmarinic acid (RA), to suppress UVB-induced (295-315 nm) alterations to human keratinocytes HaCaT using a solar simulator. Pre- and post-treatment of HaCaT cells with PVE (5-50 mg/l) and RA (0.18-1.8 mg/l) reduced breakage together with the apoptotic process. PVE and RA also significantly eliminated ROS production and diminished IL-6 release. Taken together, both PVE and RA prevent UVB-caused injury to keratinocytes. However their efficacy needs to be demonstrated in vivo.

Authors+Show Affiliations

Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hnevotínská 3, Olomouc, Czech Republic.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19862537

Citation

Vostálová, Jitka, et al. "Prunella Vulgaris Extract and Rosmarinic Acid Prevent UVB-induced DNA Damage and Oxidative Stress in HaCaT Keratinocytes." Archives of Dermatological Research, vol. 302, no. 3, 2010, pp. 171-81.
Vostálová J, Zdarilová A, Svobodová A. Prunella vulgaris extract and rosmarinic acid prevent UVB-induced DNA damage and oxidative stress in HaCaT keratinocytes. Arch Dermatol Res. 2010;302(3):171-81.
Vostálová, J., Zdarilová, A., & Svobodová, A. (2010). Prunella vulgaris extract and rosmarinic acid prevent UVB-induced DNA damage and oxidative stress in HaCaT keratinocytes. Archives of Dermatological Research, 302(3), 171-81. https://doi.org/10.1007/s00403-009-0999-6
Vostálová J, Zdarilová A, Svobodová A. Prunella Vulgaris Extract and Rosmarinic Acid Prevent UVB-induced DNA Damage and Oxidative Stress in HaCaT Keratinocytes. Arch Dermatol Res. 2010;302(3):171-81. PubMed PMID: 19862537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prunella vulgaris extract and rosmarinic acid prevent UVB-induced DNA damage and oxidative stress in HaCaT keratinocytes. AU - Vostálová,Jitka, AU - Zdarilová,Adéla, AU - Svobodová,Alena, Y1 - 2009/10/28/ PY - 2009/07/13/received PY - 2009/10/07/accepted PY - 2009/10/05/revised PY - 2009/10/29/entrez PY - 2009/10/29/pubmed PY - 2010/6/9/medline SP - 171 EP - 81 JF - Archives of dermatological research JO - Arch. Dermatol. Res. VL - 302 IS - 3 N2 - Solar radiation is a very important exogenous factor in skin pathogenesis and can lead to the development of a number of skin disorders. UVB irradiation is known to induce oxidative stress, inflammation and especially DNA lesions in exposed cells. It is important, therefore, to identify agents that can offer protection against UVB-caused skin damage. Natural compounds have been studied for their possible ability to control/modulate various lifestyle-related diseases. The application of plant compounds/extracts with screening, antioxidant and anti-inflammatory activities may also successfully protect the skin against UV-caused injury. We assessed the potency of Prunella vulgaris extract (PVE) and its main phenolic acid component, rosmarinic acid (RA), to suppress UVB-induced (295-315 nm) alterations to human keratinocytes HaCaT using a solar simulator. Pre- and post-treatment of HaCaT cells with PVE (5-50 mg/l) and RA (0.18-1.8 mg/l) reduced breakage together with the apoptotic process. PVE and RA also significantly eliminated ROS production and diminished IL-6 release. Taken together, both PVE and RA prevent UVB-caused injury to keratinocytes. However their efficacy needs to be demonstrated in vivo. SN - 1432-069X UR - https://www.unboundmedicine.com/medline/citation/19862537/Prunella_vulgaris_extract_and_rosmarinic_acid_prevent_UVB_induced_DNA_damage_and_oxidative_stress_in_HaCaT_keratinocytes_ L2 - https://link.springer.com/article/10.1007/s00403-009-0999-6 DB - PRIME DP - Unbound Medicine ER -