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Microdissection and chromosome painting of X and B chromosomes in the grasshopper Eyprepocnemis plorans.
Cytogenet Genome Res. 2009; 125(4):286-91.CG

Abstract

The relative location of 2 repetitive DNAs, i.e. ribosomal (rDNA) and a tandemly repeated satellite DNA (satDNA), with respect to the centromere, suggested that B chromosomes in the grasshopper Eyprepocnemis plorans derived intraspecifically from the X chromosome. To test this hypothesis, we microdissected X and B chromosomes and amplified the obtained DNA by 2 different procedures, the conventional DOP-PCR method and the single-cell whole-genome amplification GenomePlex method. We then generated DNA probes to perform chromosome painting. Our results have confirmed that X and B chromosomes share many DNA sequences between them and with most of the autosomes, especially at locations where the satDNA and rDNA reside, in consistency with previous information. This supports the hypothesis of an intraspecific origin of B chromosomes in E. plorans. Nevertheless, the present results did not help to clarify whether Bs were derived from the X chromosome or else from 1 or more autosomes.

Authors+Show Affiliations

Departamento de Genética, Universidad de Granada, Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19864892

Citation

Teruel, M, et al. "Microdissection and Chromosome Painting of X and B Chromosomes in the Grasshopper Eyprepocnemis Plorans." Cytogenetic and Genome Research, vol. 125, no. 4, 2009, pp. 286-91.
Teruel M, Cabrero J, Perfectti F, et al. Microdissection and chromosome painting of X and B chromosomes in the grasshopper Eyprepocnemis plorans. Cytogenet Genome Res. 2009;125(4):286-91.
Teruel, M., Cabrero, J., Perfectti, F., Acosta, M. J., Sánchez, A., & Camacho, J. P. (2009). Microdissection and chromosome painting of X and B chromosomes in the grasshopper Eyprepocnemis plorans. Cytogenetic and Genome Research, 125(4), 286-91. https://doi.org/10.1159/000235935
Teruel M, et al. Microdissection and Chromosome Painting of X and B Chromosomes in the Grasshopper Eyprepocnemis Plorans. Cytogenet Genome Res. 2009;125(4):286-91. PubMed PMID: 19864892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microdissection and chromosome painting of X and B chromosomes in the grasshopper Eyprepocnemis plorans. AU - Teruel,M, AU - Cabrero,J, AU - Perfectti,F, AU - Acosta,M J, AU - Sánchez,A, AU - Camacho,J P M, Y1 - 2009/10/27/ PY - 2009/06/10/accepted PY - 2009/10/30/entrez PY - 2009/10/30/pubmed PY - 2009/11/10/medline SP - 286 EP - 91 JF - Cytogenetic and genome research JO - Cytogenet. Genome Res. VL - 125 IS - 4 N2 - The relative location of 2 repetitive DNAs, i.e. ribosomal (rDNA) and a tandemly repeated satellite DNA (satDNA), with respect to the centromere, suggested that B chromosomes in the grasshopper Eyprepocnemis plorans derived intraspecifically from the X chromosome. To test this hypothesis, we microdissected X and B chromosomes and amplified the obtained DNA by 2 different procedures, the conventional DOP-PCR method and the single-cell whole-genome amplification GenomePlex method. We then generated DNA probes to perform chromosome painting. Our results have confirmed that X and B chromosomes share many DNA sequences between them and with most of the autosomes, especially at locations where the satDNA and rDNA reside, in consistency with previous information. This supports the hypothesis of an intraspecific origin of B chromosomes in E. plorans. Nevertheless, the present results did not help to clarify whether Bs were derived from the X chromosome or else from 1 or more autosomes. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/19864892/Microdissection_and_chromosome_painting_of_X_and_B_chromosomes_in_the_grasshopper_Eyprepocnemis_plorans_ L2 - https://www.karger.com?DOI=10.1159/000235935 DB - PRIME DP - Unbound Medicine ER -