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Superior: a novel repetitive DNA element dispersed in the rye genome.
Cytogenet Genome Res 2009; 125(4):306-20CG

Abstract

A new class of rye-specific repetitive DNA elements designated Superior has been identified. The rye genome library was constructed by cleavage with EcoO109I, the recognition sites of which consisted of 5'-PuGGNCCPy-3' multi-sequences and were present with high frequency in the rye repetitive families. A novel 495-bp segment enriched in the rye genome was successfully identified. Southern blot hybridization and fluorescence in situ hybridization using the repetitive element showed a dispersed array through all 7 chromosomes of rye. The repetitive DNA element did not share identity with known class I or class II transposable elements or known repetitive elements. Only several DNA segments in BACs and ESTs of barley showed partial similarity to the repetitive DNA element in all DNA databases of living species. The new class of dispersed repetitive elements was designated Superior. The entire structure of Superior was determined by using a rye genomic library of lambda FIXII screened by the 495-bp probe. The Superior family consisted of 1,292-bp, 1,324-bp, and 1,432-bp elements in which the 5' regions had been destroyed, indicating the presence of considerable structural diversity. Superior might be a useful tool for studying genomic organization and differentiation.

Authors+Show Affiliations

Molecular Genetics Laboratory, Faculty of Agriculture, Tottori University, Tottori, Japan. tomita@muses.tottori-u.ac.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19864894

Citation

Tomita, M, et al. "Superior: a Novel Repetitive DNA Element Dispersed in the Rye Genome." Cytogenetic and Genome Research, vol. 125, no. 4, 2009, pp. 306-20.
Tomita M, Kuramochi M, Iwata S. Superior: a novel repetitive DNA element dispersed in the rye genome. Cytogenet Genome Res. 2009;125(4):306-20.
Tomita, M., Kuramochi, M., & Iwata, S. (2009). Superior: a novel repetitive DNA element dispersed in the rye genome. Cytogenetic and Genome Research, 125(4), pp. 306-20. doi:10.1159/000235937.
Tomita M, Kuramochi M, Iwata S. Superior: a Novel Repetitive DNA Element Dispersed in the Rye Genome. Cytogenet Genome Res. 2009;125(4):306-20. PubMed PMID: 19864894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Superior: a novel repetitive DNA element dispersed in the rye genome. AU - Tomita,M, AU - Kuramochi,M, AU - Iwata,S, Y1 - 2009/10/27/ PY - 2009/07/06/accepted PY - 2009/10/30/entrez PY - 2009/10/30/pubmed PY - 2009/11/10/medline SP - 306 EP - 20 JF - Cytogenetic and genome research JO - Cytogenet. Genome Res. VL - 125 IS - 4 N2 - A new class of rye-specific repetitive DNA elements designated Superior has been identified. The rye genome library was constructed by cleavage with EcoO109I, the recognition sites of which consisted of 5'-PuGGNCCPy-3' multi-sequences and were present with high frequency in the rye repetitive families. A novel 495-bp segment enriched in the rye genome was successfully identified. Southern blot hybridization and fluorescence in situ hybridization using the repetitive element showed a dispersed array through all 7 chromosomes of rye. The repetitive DNA element did not share identity with known class I or class II transposable elements or known repetitive elements. Only several DNA segments in BACs and ESTs of barley showed partial similarity to the repetitive DNA element in all DNA databases of living species. The new class of dispersed repetitive elements was designated Superior. The entire structure of Superior was determined by using a rye genomic library of lambda FIXII screened by the 495-bp probe. The Superior family consisted of 1,292-bp, 1,324-bp, and 1,432-bp elements in which the 5' regions had been destroyed, indicating the presence of considerable structural diversity. Superior might be a useful tool for studying genomic organization and differentiation. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/19864894/Superior:_a_novel_repetitive_DNA_element_dispersed_in_the_rye_genome_ L2 - https://www.karger.com?DOI=10.1159/000235937 DB - PRIME DP - Unbound Medicine ER -