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Possible pathways involved in activation of catalase and superoxide dismutase with sodium nitroprusside in yeast Saccharomyces cerevisiae.
Ukr Biokhim Zh (1999). 2009 Mar-Apr; 81(2):34-9.UB

Abstract

The effect of nitric oxide (*NO) on biological systems depends very much on many circumstances. Nitric oxide can activate redox sensitive pathways that in many cases results in an increase of antioxidant potential of the cell. However, the direct effects of nitric oxide on the activity of principal antioxidant enzymes such as catalase and superoxide dismutase (SOD) have not been studied. In the present work we exploited the yeast model to elucidate a possibility of regulation of the mentioned activity by NO-donor sodium nitroprusside (SNP). We demonstrated that nitric oxide spontaneously generated at SNP decomposition increased the activity of catalase and SOD 1.3 times. Using inhibitors of mRNA (actinomycin D) and protein (cycloheximide) synthesis, the strain deficient in Yap1p, a master regulator coordinating yeast adaptive response to oxidative stress, we have found that these enzymes are up-regulated via synthesis of new molecules at transcription and translation levels. This response is mediated by Yap1p. Despite the increase of SOD activity in yeast cells possibly includes the activation of the present apoprotein by Ccs1p, the ways of nitric oxide regulation of Ccs1p activity are still unclear.

Authors+Show Affiliations

Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine. lushchak@pu.if.uaNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19873875

Citation

Lushchak, O V., and V I. Lushchak. "Possible Pathways Involved in Activation of Catalase and Superoxide Dismutase With Sodium Nitroprusside in Yeast Saccharomyces Cerevisiae." Ukrains'kyi Biokhimichnyi Zhurnal (1999), vol. 81, no. 2, 2009, pp. 34-9.
Lushchak OV, Lushchak VI. Possible pathways involved in activation of catalase and superoxide dismutase with sodium nitroprusside in yeast Saccharomyces cerevisiae. Ukr Biokhim Zh (1999). 2009;81(2):34-9.
Lushchak, O. V., & Lushchak, V. I. (2009). Possible pathways involved in activation of catalase and superoxide dismutase with sodium nitroprusside in yeast Saccharomyces cerevisiae. Ukrains'kyi Biokhimichnyi Zhurnal (1999), 81(2), 34-9.
Lushchak OV, Lushchak VI. Possible Pathways Involved in Activation of Catalase and Superoxide Dismutase With Sodium Nitroprusside in Yeast Saccharomyces Cerevisiae. Ukr Biokhim Zh (1999). 2009 Mar-Apr;81(2):34-9. PubMed PMID: 19873875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Possible pathways involved in activation of catalase and superoxide dismutase with sodium nitroprusside in yeast Saccharomyces cerevisiae. AU - Lushchak,O V, AU - Lushchak,V I, PY - 2009/10/31/entrez PY - 2009/10/31/pubmed PY - 2010/4/1/medline SP - 34 EP - 9 JF - Ukrains'kyi biokhimichnyi zhurnal (1999) JO - Ukr Biokhim Zh (1999) VL - 81 IS - 2 N2 - The effect of nitric oxide (*NO) on biological systems depends very much on many circumstances. Nitric oxide can activate redox sensitive pathways that in many cases results in an increase of antioxidant potential of the cell. However, the direct effects of nitric oxide on the activity of principal antioxidant enzymes such as catalase and superoxide dismutase (SOD) have not been studied. In the present work we exploited the yeast model to elucidate a possibility of regulation of the mentioned activity by NO-donor sodium nitroprusside (SNP). We demonstrated that nitric oxide spontaneously generated at SNP decomposition increased the activity of catalase and SOD 1.3 times. Using inhibitors of mRNA (actinomycin D) and protein (cycloheximide) synthesis, the strain deficient in Yap1p, a master regulator coordinating yeast adaptive response to oxidative stress, we have found that these enzymes are up-regulated via synthesis of new molecules at transcription and translation levels. This response is mediated by Yap1p. Despite the increase of SOD activity in yeast cells possibly includes the activation of the present apoprotein by Ccs1p, the ways of nitric oxide regulation of Ccs1p activity are still unclear. UR - https://www.unboundmedicine.com/medline/citation/19873875/Possible_pathways_involved_in_activation_of_catalase_and_superoxide_dismutase_with_sodium_nitroprusside_in_yeast_Saccharomyces_cerevisiae_ L2 - https://biocyc.org/gene?orgid=YEAST&id=YJR104C DB - PRIME DP - Unbound Medicine ER -