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Sensitive method for detection of cocaine and associated analytes by liquid chromatography-tandem mass spectrometry in urine.
J Anal Toxicol. 2009 Oct; 33(8):447-55.JA

Abstract

Cocaine (COC) is a potent CNS stimulant that is metabolized to benzoylecgonine (BE) and further metabolized to minor metabolites such as m-hydroxybenzoylecgonine (m-HOBE). COC is also metabolized to norcocaine (NC). Cocaethylene (CE) is formed when cocaine and ethyl alcohol are used simultaneously. Anhydroecgonine methyl ester (AEME) is a unique marker following smoked cocaine, and anhydroecgonine ethyl ester (AEEE) is found in cocaine smokers who also use ethyl alcohol. We developed a liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the detection and quantitation of COC, BE, NC, CE, m-HOBE, AEME, and AEEE in urine. Two hundred samples previously analyzed by gas chromatography (GC) coupled with MS were extracted using solid-phase extraction. Chromatographic separation was achieved using a gradient consisting of mobile phase A [20 mM ammonium formate (pH 2.7)] and mobile phase B (methanol/acetonitrile, 50:50), an XDB-C(8) (50 x 2.1 mm, 1.8 microm) column and a flow rate of 270 microL/min. Concentrations were calculated by comparing the peak-area with the internal standard and plotted against a standard curve. The assay displayed linearity from 1.0 to 100 ng/mL. Within- and between-run coefficients of variation were < 10% throughout the linear range. A method comparison between GC-MS and LC-MS-MS showed good correlation for COC (r(2) = 0.982) and BE (r(2) = 0.955). We report here on a sensitive method to identify clinically and forensically relevant cocaine and associated analytes at concentrations as low as 1.0 ng/mL.

Authors+Show Affiliations

Toxicology and Drug Monitoring Laboratory, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA. Langman.loralie@mayo.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19874651

Citation

Langman, Loralie J., et al. "Sensitive Method for Detection of Cocaine and Associated Analytes By Liquid Chromatography-tandem Mass Spectrometry in Urine." Journal of Analytical Toxicology, vol. 33, no. 8, 2009, pp. 447-55.
Langman LJ, Bjergum MW, Williamson CL, et al. Sensitive method for detection of cocaine and associated analytes by liquid chromatography-tandem mass spectrometry in urine. J Anal Toxicol. 2009;33(8):447-55.
Langman, L. J., Bjergum, M. W., Williamson, C. L., & Crow, F. W. (2009). Sensitive method for detection of cocaine and associated analytes by liquid chromatography-tandem mass spectrometry in urine. Journal of Analytical Toxicology, 33(8), 447-55.
Langman LJ, et al. Sensitive Method for Detection of Cocaine and Associated Analytes By Liquid Chromatography-tandem Mass Spectrometry in Urine. J Anal Toxicol. 2009;33(8):447-55. PubMed PMID: 19874651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive method for detection of cocaine and associated analytes by liquid chromatography-tandem mass spectrometry in urine. AU - Langman,Loralie J, AU - Bjergum,Matthew W, AU - Williamson,Christopher L, AU - Crow,Frank W, PY - 2009/10/31/entrez PY - 2009/10/31/pubmed PY - 2010/1/6/medline SP - 447 EP - 55 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 33 IS - 8 N2 - Cocaine (COC) is a potent CNS stimulant that is metabolized to benzoylecgonine (BE) and further metabolized to minor metabolites such as m-hydroxybenzoylecgonine (m-HOBE). COC is also metabolized to norcocaine (NC). Cocaethylene (CE) is formed when cocaine and ethyl alcohol are used simultaneously. Anhydroecgonine methyl ester (AEME) is a unique marker following smoked cocaine, and anhydroecgonine ethyl ester (AEEE) is found in cocaine smokers who also use ethyl alcohol. We developed a liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the detection and quantitation of COC, BE, NC, CE, m-HOBE, AEME, and AEEE in urine. Two hundred samples previously analyzed by gas chromatography (GC) coupled with MS were extracted using solid-phase extraction. Chromatographic separation was achieved using a gradient consisting of mobile phase A [20 mM ammonium formate (pH 2.7)] and mobile phase B (methanol/acetonitrile, 50:50), an XDB-C(8) (50 x 2.1 mm, 1.8 microm) column and a flow rate of 270 microL/min. Concentrations were calculated by comparing the peak-area with the internal standard and plotted against a standard curve. The assay displayed linearity from 1.0 to 100 ng/mL. Within- and between-run coefficients of variation were < 10% throughout the linear range. A method comparison between GC-MS and LC-MS-MS showed good correlation for COC (r(2) = 0.982) and BE (r(2) = 0.955). We report here on a sensitive method to identify clinically and forensically relevant cocaine and associated analytes at concentrations as low as 1.0 ng/mL. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/19874651/Sensitive_method_for_detection_of_cocaine_and_associated_analytes_by_liquid_chromatography_tandem_mass_spectrometry_in_urine_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/33.8.447 DB - PRIME DP - Unbound Medicine ER -