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Characterization of the polyphenolic fraction of Morus alba leaves extracts by HPLC coupled to a hybrid IT-TOF MS system.
J Sep Sci. 2009 Nov; 32(21):3627-34.JS

Abstract

Infusions or dried leaves from Morus alba (L., Moraceae) have long been used in folk medicine for the prevention or treatment of several diseases. Despite the great interest in determining the role of phytonutrients as potential therapeutic agents, and the rising demand of natural sources with nutraceutic benefits, the antioxidant content of many foodstuffs is unknown, making accurate estimation for human dietary consumption and the correlation to human diseases difficult. The high content in polyphenols mainly accounts for in vitro and in vivo antioxidant activity of extracts obtained from this plant, and it is responsible for their preventive and therapeutic roles in a number of human diseases. In this study, we achieved full characterization and quantitation of flavonoids and other phenolic components extracted from lyophilized mulberry leaves, by maceration with ethanol. To tackle such a task, an RPLC-diode array detector-MS system was optimized, employing a partially porous octadecylsilica column as stationary phase. Identification of mulberry leaves constituents was carried out on the basis of the complementary information obtained from their migration times, diode array spectra, MS ions, and MS/MS fragments. The employment of the hybrid (IT-TOF) mass spectrometer further allowed the structural assignment of a series of structural isomers. To the best of our knowledge, 11 out of 22 identified compounds are being reported in mulberry leaves for the first time, and the relative amounts determined, in the two cultivars analyzed.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Dugo P
Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy. pdugo@pharma.unime.it
Donato P
No affiliation info available
Cacciola F
No affiliation info available
Germanò MP
No affiliation info available
Rapisarda A
No affiliation info available
Mondello L
No affiliation info available

MeSH

Chromatography, High Pressure LiquidFlavonoidsMass SpectrometryMorusPhenolsPlant ExtractsPlant LeavesPolyphenols

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19877143

Citation

Dugo, Paola, et al. "Characterization of the Polyphenolic Fraction of Morus Alba Leaves Extracts By HPLC Coupled to a Hybrid IT-TOF MS System." Journal of Separation Science, vol. 32, no. 21, 2009, pp. 3627-34.
Dugo P, Donato P, Cacciola F, et al. Characterization of the polyphenolic fraction of Morus alba leaves extracts by HPLC coupled to a hybrid IT-TOF MS system. J Sep Sci. 2009;32(21):3627-34.
Dugo, P., Donato, P., Cacciola, F., Germanò, M. P., Rapisarda, A., & Mondello, L. (2009). Characterization of the polyphenolic fraction of Morus alba leaves extracts by HPLC coupled to a hybrid IT-TOF MS system. Journal of Separation Science, 32(21), 3627-34. https://doi.org/10.1002/jssc.200900348
Dugo P, et al. Characterization of the Polyphenolic Fraction of Morus Alba Leaves Extracts By HPLC Coupled to a Hybrid IT-TOF MS System. J Sep Sci. 2009;32(21):3627-34. PubMed PMID: 19877143.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the polyphenolic fraction of Morus alba leaves extracts by HPLC coupled to a hybrid IT-TOF MS system. AU - Dugo,Paola, AU - Donato,Paola, AU - Cacciola,Francesco, AU - Germanò,Maria Paola, AU - Rapisarda,Antonio, AU - Mondello,Luigi, PY - 2009/10/31/entrez PY - 2009/10/31/pubmed PY - 2010/3/2/medline SP - 3627 EP - 34 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 21 N2 - Infusions or dried leaves from Morus alba (L., Moraceae) have long been used in folk medicine for the prevention or treatment of several diseases. Despite the great interest in determining the role of phytonutrients as potential therapeutic agents, and the rising demand of natural sources with nutraceutic benefits, the antioxidant content of many foodstuffs is unknown, making accurate estimation for human dietary consumption and the correlation to human diseases difficult. The high content in polyphenols mainly accounts for in vitro and in vivo antioxidant activity of extracts obtained from this plant, and it is responsible for their preventive and therapeutic roles in a number of human diseases. In this study, we achieved full characterization and quantitation of flavonoids and other phenolic components extracted from lyophilized mulberry leaves, by maceration with ethanol. To tackle such a task, an RPLC-diode array detector-MS system was optimized, employing a partially porous octadecylsilica column as stationary phase. Identification of mulberry leaves constituents was carried out on the basis of the complementary information obtained from their migration times, diode array spectra, MS ions, and MS/MS fragments. The employment of the hybrid (IT-TOF) mass spectrometer further allowed the structural assignment of a series of structural isomers. To the best of our knowledge, 11 out of 22 identified compounds are being reported in mulberry leaves for the first time, and the relative amounts determined, in the two cultivars analyzed. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/19877143/Characterization_of_the_polyphenolic_fraction_of_Morus_alba_leaves_extracts_by_HPLC_coupled_to_a_hybrid_IT_TOF_MS_system_ L2 - https://doi.org/10.1002/jssc.200900348 DB - PRIME DP - Unbound Medicine ER -
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