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Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group.
Environ Microbiol. 2010 Feb; 12(2):422-39.EM

Abstract

Microbial consortia mediating the anaerobic oxidation of methane with sulfate are composed of methanotrophic Archaea (ANME) and Bacteria related to sulfate-reducing Deltaproteobacteria. Cultured representatives are not available for any of the three ANME clades. Therefore, a metagenomic approach was applied to assess the genetic potential of ANME-1 archaea. In total, 3.4 Mbp sequence information was generated based on metagenomic fosmid libraries constructed directly from a methanotrophic microbial mat in the Black Sea. These sequence data represent, in 30 contigs, about 82-90% of a composite ANME-1 genome. The dataset supports the hypothesis of a reversal of the methanogenesis pathway. Indications for an assimilatory, but not for a dissimilatory sulfate reduction pathway in ANME-1, were found. Draft genome and expression analyses are consistent with acetate and formate as putative electron shuttles. Moreover, the dataset points towards downstream electron-accepting redox components different from the ones known from methanogenic archaea. Whereas catalytic subunits of [NiFe]-hydrogenases are lacking in the dataset, genes for an [FeFe]-hydrogenase homologue were identified, not yet described to be present in methanogenic archaea. Clustered genes annotated as secreted multiheme c-type cytochromes were identified, which have not yet been correlated with methanogenesis-related steps. The genes were shown to be expressed, suggesting direct electron transfer as an additional possible mode to shuttle electrons from ANME-1 to the bacterial sulfate-reducing partner.

Authors+Show Affiliations

Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany. ameyerdi@mpi-bremen.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19878267

Citation

Meyerdierks, Anke, et al. "Metagenome and mRNA Expression Analyses of Anaerobic Methanotrophic Archaea of the ANME-1 Group." Environmental Microbiology, vol. 12, no. 2, 2010, pp. 422-39.
Meyerdierks A, Kube M, Kostadinov I, et al. Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group. Environ Microbiol. 2010;12(2):422-39.
Meyerdierks, A., Kube, M., Kostadinov, I., Teeling, H., Glöckner, F. O., Reinhardt, R., & Amann, R. (2010). Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group. Environmental Microbiology, 12(2), 422-39. https://doi.org/10.1111/j.1462-2920.2009.02083.x
Meyerdierks A, et al. Metagenome and mRNA Expression Analyses of Anaerobic Methanotrophic Archaea of the ANME-1 Group. Environ Microbiol. 2010;12(2):422-39. PubMed PMID: 19878267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group. AU - Meyerdierks,Anke, AU - Kube,Michael, AU - Kostadinov,Ivaylo, AU - Teeling,Hanno, AU - Glöckner,Frank Oliver, AU - Reinhardt,Richard, AU - Amann,Rudolf, Y1 - 2009/10/29/ PY - 2009/11/3/entrez PY - 2009/11/3/pubmed PY - 2010/7/16/medline SP - 422 EP - 39 JF - Environmental microbiology JO - Environ. Microbiol. VL - 12 IS - 2 N2 - Microbial consortia mediating the anaerobic oxidation of methane with sulfate are composed of methanotrophic Archaea (ANME) and Bacteria related to sulfate-reducing Deltaproteobacteria. Cultured representatives are not available for any of the three ANME clades. Therefore, a metagenomic approach was applied to assess the genetic potential of ANME-1 archaea. In total, 3.4 Mbp sequence information was generated based on metagenomic fosmid libraries constructed directly from a methanotrophic microbial mat in the Black Sea. These sequence data represent, in 30 contigs, about 82-90% of a composite ANME-1 genome. The dataset supports the hypothesis of a reversal of the methanogenesis pathway. Indications for an assimilatory, but not for a dissimilatory sulfate reduction pathway in ANME-1, were found. Draft genome and expression analyses are consistent with acetate and formate as putative electron shuttles. Moreover, the dataset points towards downstream electron-accepting redox components different from the ones known from methanogenic archaea. Whereas catalytic subunits of [NiFe]-hydrogenases are lacking in the dataset, genes for an [FeFe]-hydrogenase homologue were identified, not yet described to be present in methanogenic archaea. Clustered genes annotated as secreted multiheme c-type cytochromes were identified, which have not yet been correlated with methanogenesis-related steps. The genes were shown to be expressed, suggesting direct electron transfer as an additional possible mode to shuttle electrons from ANME-1 to the bacterial sulfate-reducing partner. SN - 1462-2920 UR - https://www.unboundmedicine.com/medline/citation/19878267/Metagenome_and_mRNA_expression_analyses_of_anaerobic_methanotrophic_archaea_of_the_ANME_1_group_ L2 - https://doi.org/10.1111/j.1462-2920.2009.02083.x DB - PRIME DP - Unbound Medicine ER -