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Upregulation of stromal cell derived factor-1alpha in collagen vascular diseases-associated interstitial pneumonias (CVDs-IPs).
Pulm Pharmacol Ther. 2010 Apr; 23(2):115-20.PP

Abstract

OBJECTIVE

We speculated that distinct angiogenic profiles are involved in idiopathic interstitial pneumonias (IIPs) in comparison with interstitial pneumonias associated with collagen vascular disease (CVD-IPs). This hypothesis was investigated by measuring the expression of a cardinal biologic axis, the vascular endothelial growth factor (VEGF)-stromal derived growth factor [SDF-1alpha, transcripts 1 and 2 (TR1 and TR2)] and receptor, CXCR4 and the angiogenetic receptors CXCR2 and CXCR3 in bronchoalveolar lavage fluid (BALF) in both conditions.

METHODS

We studied prospectively 25 patients with fibrotic IIPs (f-IIPs) [20 with idiopathic pulmonary fibrosis (IPF) and 5 with idiopathic non-specific interstitial pneumonia (NSIP)] and 16 patients with CVD-IPs. mRNA expression was measured by Real-Time RT-PCR and protein was evaluated by Western Blotting.

RESULTS

A significantly greater value has been detected in SDF-1alpha-TR1 mRNA expression levels of CVD-IPs (p=0.05) in comparison with IPF group. A similar trend has been also detected in protein expression in favor of CVD-IP group. In addition, VEGF mRNA levels have been found significantly increased in CVD-IPs in comparison with the NSIP group (p=0.05). No significant difference has been found in SDF-1alpha-TR2-CXCR4 mRNA and CXCR2-CXCR3 between the two groups.

CONCLUSION

These results showed increased expression of SDF-1alpha in CVD-IPs, suggesting different angiogenic procedures. Further studies are needed in order to better explore the angiogenetic pathway in these disorders.

Authors+Show Affiliations

Department of Thoracic Medicine, Interstitial Lung Disease Unit, University Hospital, Medical School, University of Crete, Heraklion 71110, Crete, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19878731

Citation

Margaritopoulos, Giorgos A., et al. "Upregulation of Stromal Cell Derived Factor-1alpha in Collagen Vascular Diseases-associated Interstitial Pneumonias (CVDs-IPs)." Pulmonary Pharmacology & Therapeutics, vol. 23, no. 2, 2010, pp. 115-20.
Margaritopoulos GA, Antoniou KM, Soufla G, et al. Upregulation of stromal cell derived factor-1alpha in collagen vascular diseases-associated interstitial pneumonias (CVDs-IPs). Pulm Pharmacol Ther. 2010;23(2):115-20.
Margaritopoulos, G. A., Antoniou, K. M., Soufla, G., Karagiannis, K., Proklou, A., Lasithiotaki, I., Tzanakis, N., Spandidos, D. A., & Siafakas, N. M. (2010). Upregulation of stromal cell derived factor-1alpha in collagen vascular diseases-associated interstitial pneumonias (CVDs-IPs). Pulmonary Pharmacology & Therapeutics, 23(2), 115-20. https://doi.org/10.1016/j.pupt.2009.10.008
Margaritopoulos GA, et al. Upregulation of Stromal Cell Derived Factor-1alpha in Collagen Vascular Diseases-associated Interstitial Pneumonias (CVDs-IPs). Pulm Pharmacol Ther. 2010;23(2):115-20. PubMed PMID: 19878731.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Upregulation of stromal cell derived factor-1alpha in collagen vascular diseases-associated interstitial pneumonias (CVDs-IPs). AU - Margaritopoulos,Giorgos A, AU - Antoniou,Katerina M, AU - Soufla,Giannoula, AU - Karagiannis,Konstantinos, AU - Proklou,Athanasia, AU - Lasithiotaki,Ismini, AU - Tzanakis,Nikolaos, AU - Spandidos,Demetrios A, AU - Siafakas,Nikolaos M, Y1 - 2009/10/28/ PY - 2009/05/19/received PY - 2009/10/08/revised PY - 2009/10/21/accepted PY - 2009/11/3/entrez PY - 2009/11/3/pubmed PY - 2010/5/4/medline SP - 115 EP - 20 JF - Pulmonary pharmacology & therapeutics JO - Pulm Pharmacol Ther VL - 23 IS - 2 N2 - OBJECTIVE: We speculated that distinct angiogenic profiles are involved in idiopathic interstitial pneumonias (IIPs) in comparison with interstitial pneumonias associated with collagen vascular disease (CVD-IPs). This hypothesis was investigated by measuring the expression of a cardinal biologic axis, the vascular endothelial growth factor (VEGF)-stromal derived growth factor [SDF-1alpha, transcripts 1 and 2 (TR1 and TR2)] and receptor, CXCR4 and the angiogenetic receptors CXCR2 and CXCR3 in bronchoalveolar lavage fluid (BALF) in both conditions. METHODS: We studied prospectively 25 patients with fibrotic IIPs (f-IIPs) [20 with idiopathic pulmonary fibrosis (IPF) and 5 with idiopathic non-specific interstitial pneumonia (NSIP)] and 16 patients with CVD-IPs. mRNA expression was measured by Real-Time RT-PCR and protein was evaluated by Western Blotting. RESULTS: A significantly greater value has been detected in SDF-1alpha-TR1 mRNA expression levels of CVD-IPs (p=0.05) in comparison with IPF group. A similar trend has been also detected in protein expression in favor of CVD-IP group. In addition, VEGF mRNA levels have been found significantly increased in CVD-IPs in comparison with the NSIP group (p=0.05). No significant difference has been found in SDF-1alpha-TR2-CXCR4 mRNA and CXCR2-CXCR3 between the two groups. CONCLUSION: These results showed increased expression of SDF-1alpha in CVD-IPs, suggesting different angiogenic procedures. Further studies are needed in order to better explore the angiogenetic pathway in these disorders. SN - 1522-9629 UR - https://www.unboundmedicine.com/medline/citation/19878731/Upregulation_of_stromal_cell_derived_factor_1alpha_in_collagen_vascular_diseases_associated_interstitial_pneumonias__CVDs_IPs__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1094-5539(09)00122-9 DB - PRIME DP - Unbound Medicine ER -