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Cardiac fibroblasts have functional TRPV4 activated by 4alpha-phorbol 12,13-didecanoate.
Life Sci. 2009 Dec 16; 85(23-26):808-14.LS

Abstract

AIMS

Vanilloid type transient receptor potential channel (TRPV) could be a potential environmental sensor to multiple stimuli in many types of cells. In this study, we provide the first evidence of functional vanilloid type 4 transient receptor potential channel (TRPV4) in rat cardiac fibroblasts (CFs).

MAIN METHODS

Expression of TRPV4 in CFs was analyzed at mRNA and protein level. Function of TRPV4 in CFs was evaluated using a selective TRPV4 agonist, 4alpha-phorbol 12,13-didecanoate (4alphaPDD) while measuring intracellular Ca(2+) concentration ([Ca(2+)](i)) and membrane currents.

KEY FINDINGS

Analysis of expression of mRNA transcripts of TRPV subfamily revealed that TRPV2 and TRPV4 were expressed in CFs. Significant immunoreactivity to TRPV4 protein was also detected in CFs. When 4alphaPDD was applied to CFs, [Ca(2+)](i) was elevated in a concentration-dependent manner. The elevation of [Ca(2+)](i) was abolished by the removal of external Ca(2+) and by ruthenium red (RuR). 4alphaPDD also activated non-selective cation currents (NSCCs), which were suppressed by RuR. Moreover, pretreatment of CFs with short interference RNA (siRNA) targeting TRPV4 significantly reduced both 4alphaPDD-induced elevation of [Ca(2+)](i) and NSCC.

SIGNIFICANCE

These results provide strong evidence that endogenous TRPV4 functions as an important regulator of [Ca(2+)](i) in CFs.

Authors+Show Affiliations

Laboratory of Cellular Pharmacology, School of Pharmacy, Aichi-Gakuin University, 1-100 Kusumoto, Chikusa, Nagoya 464-8650, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19879881

Citation

Hatano, Noriyuki, et al. "Cardiac Fibroblasts Have Functional TRPV4 Activated By 4alpha-phorbol 12,13-didecanoate." Life Sciences, vol. 85, no. 23-26, 2009, pp. 808-14.
Hatano N, Itoh Y, Muraki K. Cardiac fibroblasts have functional TRPV4 activated by 4alpha-phorbol 12,13-didecanoate. Life Sci. 2009;85(23-26):808-14.
Hatano, N., Itoh, Y., & Muraki, K. (2009). Cardiac fibroblasts have functional TRPV4 activated by 4alpha-phorbol 12,13-didecanoate. Life Sciences, 85(23-26), 808-14. https://doi.org/10.1016/j.lfs.2009.10.013
Hatano N, Itoh Y, Muraki K. Cardiac Fibroblasts Have Functional TRPV4 Activated By 4alpha-phorbol 12,13-didecanoate. Life Sci. 2009 Dec 16;85(23-26):808-14. PubMed PMID: 19879881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cardiac fibroblasts have functional TRPV4 activated by 4alpha-phorbol 12,13-didecanoate. AU - Hatano,Noriyuki, AU - Itoh,Yuka, AU - Muraki,Katsuhiko, Y1 - 2009/10/30/ PY - 2009/05/13/received PY - 2009/10/17/revised PY - 2009/10/21/accepted PY - 2009/11/3/entrez PY - 2009/11/3/pubmed PY - 2009/12/16/medline SP - 808 EP - 14 JF - Life sciences JO - Life Sci VL - 85 IS - 23-26 N2 - AIMS: Vanilloid type transient receptor potential channel (TRPV) could be a potential environmental sensor to multiple stimuli in many types of cells. In this study, we provide the first evidence of functional vanilloid type 4 transient receptor potential channel (TRPV4) in rat cardiac fibroblasts (CFs). MAIN METHODS: Expression of TRPV4 in CFs was analyzed at mRNA and protein level. Function of TRPV4 in CFs was evaluated using a selective TRPV4 agonist, 4alpha-phorbol 12,13-didecanoate (4alphaPDD) while measuring intracellular Ca(2+) concentration ([Ca(2+)](i)) and membrane currents. KEY FINDINGS: Analysis of expression of mRNA transcripts of TRPV subfamily revealed that TRPV2 and TRPV4 were expressed in CFs. Significant immunoreactivity to TRPV4 protein was also detected in CFs. When 4alphaPDD was applied to CFs, [Ca(2+)](i) was elevated in a concentration-dependent manner. The elevation of [Ca(2+)](i) was abolished by the removal of external Ca(2+) and by ruthenium red (RuR). 4alphaPDD also activated non-selective cation currents (NSCCs), which were suppressed by RuR. Moreover, pretreatment of CFs with short interference RNA (siRNA) targeting TRPV4 significantly reduced both 4alphaPDD-induced elevation of [Ca(2+)](i) and NSCC. SIGNIFICANCE: These results provide strong evidence that endogenous TRPV4 functions as an important regulator of [Ca(2+)](i) in CFs. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/19879881/Cardiac_fibroblasts_have_functional_TRPV4_activated_by_4alpha_phorbol_1213_didecanoate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(09)00438-X DB - PRIME DP - Unbound Medicine ER -