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Low-density macroarray targeting non-locus of enterocyte effacement effectors (nle genes) and major virulence factors of Shiga toxin-producing Escherichia coli (STEC): a new approach for molecular risk assessment of STEC isolates.
Appl Environ Microbiol. 2010 Jan; 76(1):203-11.AE

Abstract

Rapid and specific detection of Shiga toxin-producing Escherichia coli (STEC) strains with a high level of virulence for humans has become a priority for public health authorities. This study reports on the development of a low-density macroarray for simultaneously testing the genes stx1, stx2, eae, and ehxA and six different nle genes issued from genomic islands OI-122 (ent, nleB, and nleE) and OI-71 (nleF, nleH1-2, and nleA). Various strains of E. coli isolated from the environment, food, animals, and healthy children have been compared with clinical isolates of various seropathotypes. The eae gene was detected in all enteropathogenic E. coli (EPEC) strains as well as in enterohemorrhagic E. coli (EHEC) strains, except in EHEC O91:H21 and EHEC O113:H21. The gene ehxA was more prevalent in EHEC (90%) than in STEC (42.66%) strains, in which it was unequally distributed. The nle genes were detected only in some EPEC and EHEC strains but with various distributions, showing that nle genes are strain and/or serotype specific, probably reflecting adaptation of the strains to different hosts or environmental niches. One characteristic nle gene distribution in EHEC O157:[H7], O111:[H8], O26:[H11], O103:H25, O118:[H16], O121:[H19], O5:H-, O55:H7, O123:H11, O172:H25, and O165:H25 was ent/espL2, nleB, nleE, nleF, nleH1-2, nleA. (Brackets indicate genotyping of the flic or rfb genes.) A second nle pattern (ent/espL2, nleB, nleE, nleH1-2) was characteristic of EHEC O103:H2, O145:[H28], O45:H2, and O15:H2. The presence of eae, ent/espL2, nleB, nleE, and nleH1-2 genes is a clear signature of STEC strains with high virulence for humans.

Authors+Show Affiliations

French Food Safety Agency, Food Quality and Agro-alimentary Processes Research Laboratory, Avenue du Général De Gaulle, F-94706 Maisons-Alfort, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19880649

Citation

Bugarel, Marie, et al. "Low-density Macroarray Targeting Non-locus of Enterocyte Effacement Effectors (nle Genes) and Major Virulence Factors of Shiga Toxin-producing Escherichia Coli (STEC): a New Approach for Molecular Risk Assessment of STEC Isolates." Applied and Environmental Microbiology, vol. 76, no. 1, 2010, pp. 203-11.
Bugarel M, Beutin L, Fach P. Low-density macroarray targeting non-locus of enterocyte effacement effectors (nle genes) and major virulence factors of Shiga toxin-producing Escherichia coli (STEC): a new approach for molecular risk assessment of STEC isolates. Appl Environ Microbiol. 2010;76(1):203-11.
Bugarel, M., Beutin, L., & Fach, P. (2010). Low-density macroarray targeting non-locus of enterocyte effacement effectors (nle genes) and major virulence factors of Shiga toxin-producing Escherichia coli (STEC): a new approach for molecular risk assessment of STEC isolates. Applied and Environmental Microbiology, 76(1), 203-11. https://doi.org/10.1128/AEM.01921-09
Bugarel M, Beutin L, Fach P. Low-density Macroarray Targeting Non-locus of Enterocyte Effacement Effectors (nle Genes) and Major Virulence Factors of Shiga Toxin-producing Escherichia Coli (STEC): a New Approach for Molecular Risk Assessment of STEC Isolates. Appl Environ Microbiol. 2010;76(1):203-11. PubMed PMID: 19880649.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Low-density macroarray targeting non-locus of enterocyte effacement effectors (nle genes) and major virulence factors of Shiga toxin-producing Escherichia coli (STEC): a new approach for molecular risk assessment of STEC isolates. AU - Bugarel,Marie, AU - Beutin,Lothar, AU - Fach,Patrick, Y1 - 2009/10/30/ PY - 2009/11/3/entrez PY - 2009/11/3/pubmed PY - 2010/2/27/medline SP - 203 EP - 11 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 76 IS - 1 N2 - Rapid and specific detection of Shiga toxin-producing Escherichia coli (STEC) strains with a high level of virulence for humans has become a priority for public health authorities. This study reports on the development of a low-density macroarray for simultaneously testing the genes stx1, stx2, eae, and ehxA and six different nle genes issued from genomic islands OI-122 (ent, nleB, and nleE) and OI-71 (nleF, nleH1-2, and nleA). Various strains of E. coli isolated from the environment, food, animals, and healthy children have been compared with clinical isolates of various seropathotypes. The eae gene was detected in all enteropathogenic E. coli (EPEC) strains as well as in enterohemorrhagic E. coli (EHEC) strains, except in EHEC O91:H21 and EHEC O113:H21. The gene ehxA was more prevalent in EHEC (90%) than in STEC (42.66%) strains, in which it was unequally distributed. The nle genes were detected only in some EPEC and EHEC strains but with various distributions, showing that nle genes are strain and/or serotype specific, probably reflecting adaptation of the strains to different hosts or environmental niches. One characteristic nle gene distribution in EHEC O157:[H7], O111:[H8], O26:[H11], O103:H25, O118:[H16], O121:[H19], O5:H-, O55:H7, O123:H11, O172:H25, and O165:H25 was ent/espL2, nleB, nleE, nleF, nleH1-2, nleA. (Brackets indicate genotyping of the flic or rfb genes.) A second nle pattern (ent/espL2, nleB, nleE, nleH1-2) was characteristic of EHEC O103:H2, O145:[H28], O45:H2, and O15:H2. The presence of eae, ent/espL2, nleB, nleE, and nleH1-2 genes is a clear signature of STEC strains with high virulence for humans. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/19880649/Low_density_macroarray_targeting_non_locus_of_enterocyte_effacement_effectors__nle_genes__and_major_virulence_factors_of_Shiga_toxin_producing_Escherichia_coli__STEC_:_a_new_approach_for_molecular_risk_assessment_of_STEC_isolates_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=19880649 DB - PRIME DP - Unbound Medicine ER -