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Measurement of interleukin-6 in bronchoalveolar lavage fluid by radioimmunoassay: differences between patients with interstitial lung disease and control subjects.
Clin Exp Immunol. 1991 Jan; 83(1):30-4.CE

Abstract

Bronchoalveolar lavage fluid (BALF) from subjects with a variety of interstitial lung diseases (active sarcoidosis, pigeon breeders' disease (PBD), asymptomatic pigeon breeders, patients with idiopathic pulmonary fibrosis) and from control subjects were assayed for interleukin-6 (IL-6) using a novel radioimmunoassay system. IL-6 was detectable in BALF from all groups, with disease groups showing significantly increased IL-6 levels compared with controls (P less than 0.01 in all cases). When these results were standardized, using urea to compensate for dilution effects in the BALF, only the asymptomatic pigeon breeders had significantly higher IL-6 levels than the controls (P less than 0.025), with all other groups showing no difference. When albumin was used for standardization, both the PBD group (P less than 0.001) and the sarcoidosis patients (P less than 0.01) had considerably lower levels of IL-6 than the control subjects. Using either albumin or urea for standardization, the PBD patients had significantly lower levels of IL-6 than do their asymptomatic counterparts (P less than 0.001 in both cases). This is contrasted by the finding of greatly elevated levels of IgG in the BALF of the PBD patients compared with asymptomatics (P less than 0.001). There was, however, no relation between IL-6 and IgG in any patient group, although the PBD patients had the lowest IL-6 and highest IgG as a group. These findings may suggest a mechanism by which asymptomatic subjects remain free from clinical complaints.

Authors+Show Affiliations

MRC, Department of Medical Microbiology, University of Wales College of Medicine, Heath Park, Cardiff, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1988228

Citation

Jones, K P., et al. "Measurement of Interleukin-6 in Bronchoalveolar Lavage Fluid By Radioimmunoassay: Differences Between Patients With Interstitial Lung Disease and Control Subjects." Clinical and Experimental Immunology, vol. 83, no. 1, 1991, pp. 30-4.
Jones KP, Reynolds SP, Capper SJ, et al. Measurement of interleukin-6 in bronchoalveolar lavage fluid by radioimmunoassay: differences between patients with interstitial lung disease and control subjects. Clin Exp Immunol. 1991;83(1):30-4.
Jones, K. P., Reynolds, S. P., Capper, S. J., Kalinka, S., Edwards, J. H., & Davies, B. H. (1991). Measurement of interleukin-6 in bronchoalveolar lavage fluid by radioimmunoassay: differences between patients with interstitial lung disease and control subjects. Clinical and Experimental Immunology, 83(1), 30-4.
Jones KP, et al. Measurement of Interleukin-6 in Bronchoalveolar Lavage Fluid By Radioimmunoassay: Differences Between Patients With Interstitial Lung Disease and Control Subjects. Clin Exp Immunol. 1991;83(1):30-4. PubMed PMID: 1988228.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Measurement of interleukin-6 in bronchoalveolar lavage fluid by radioimmunoassay: differences between patients with interstitial lung disease and control subjects. AU - Jones,K P, AU - Reynolds,S P, AU - Capper,S J, AU - Kalinka,S, AU - Edwards,J H, AU - Davies,B H, PY - 1991/1/1/pubmed PY - 1991/1/1/medline PY - 1991/1/1/entrez SP - 30 EP - 4 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 83 IS - 1 N2 - Bronchoalveolar lavage fluid (BALF) from subjects with a variety of interstitial lung diseases (active sarcoidosis, pigeon breeders' disease (PBD), asymptomatic pigeon breeders, patients with idiopathic pulmonary fibrosis) and from control subjects were assayed for interleukin-6 (IL-6) using a novel radioimmunoassay system. IL-6 was detectable in BALF from all groups, with disease groups showing significantly increased IL-6 levels compared with controls (P less than 0.01 in all cases). When these results were standardized, using urea to compensate for dilution effects in the BALF, only the asymptomatic pigeon breeders had significantly higher IL-6 levels than the controls (P less than 0.025), with all other groups showing no difference. When albumin was used for standardization, both the PBD group (P less than 0.001) and the sarcoidosis patients (P less than 0.01) had considerably lower levels of IL-6 than the control subjects. Using either albumin or urea for standardization, the PBD patients had significantly lower levels of IL-6 than do their asymptomatic counterparts (P less than 0.001 in both cases). This is contrasted by the finding of greatly elevated levels of IgG in the BALF of the PBD patients compared with asymptomatics (P less than 0.001). There was, however, no relation between IL-6 and IgG in any patient group, although the PBD patients had the lowest IL-6 and highest IgG as a group. These findings may suggest a mechanism by which asymptomatic subjects remain free from clinical complaints. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/1988228/Measurement_of_interleukin_6_in_bronchoalveolar_lavage_fluid_by_radioimmunoassay:_differences_between_patients_with_interstitial_lung_disease_and_control_subjects_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0009-9104&date=1991&volume=83&issue=1&spage=30 DB - PRIME DP - Unbound Medicine ER -