Tags

Type your tag names separated by a space and hit enter

Neuroprotection of midbrain dopaminergic cells in MPTP-treated mice after near-infrared light treatment.
J Comp Neurol. 2010 Jan 01; 518(1):25-40.JC

Abstract

This study explores whether near-infrared (NIr) light treatment neuroprotects dopaminergic cells in the substantia nigra pars compacta (SNc) and the zona incerta-hypothalamus (ZI-Hyp) from degeneration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. BALB/c albino mice were divided into four groups: 1) Saline, 2) Saline-NIr, 3) MPTP, 4) MPTP-NIr. The injections were intraperitoneal and they were followed immediately by NIr light treatment (or not). Two doses of MPTP, mild (50 mg/kg) and strong (100 mg/kg), were used. Mice were perfused transcardially with aldehyde fixative 6 days after their MPTP treatment. Brains were processed for tyrosine hydroxylase (TH) immunochemistry. The number of TH(+) cells was estimated using the optical fractionator method. Our major finding was that in the SNc there were significantly more dopaminergic cells in the MPTP-NIr compared to the MPTP group (35%-45%). By contrast, in the ZI-Hyp there was no significant difference in the numbers of cells in these two groups. In addition, our results indicated that survival in the two regions after MPTP insult was dose-dependent. In the stronger MPTP regime, the magnitude of loss was similar in the two regions (approximately 60%), while in the milder regime cell loss was greater in the SNc (45%) than ZI-Hyp (approximately 30%). In summary, our results indicate that NIr light treatment offers neuroprotection against MPTP toxicity for dopaminergic cells in the SNc, but not in the ZI-Hyp.

Authors+Show Affiliations

Discipline of Anatomy & Histology F13, University of Sydney, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19882716

Citation

Shaw, Victoria E., et al. "Neuroprotection of Midbrain Dopaminergic Cells in MPTP-treated Mice After Near-infrared Light Treatment." The Journal of Comparative Neurology, vol. 518, no. 1, 2010, pp. 25-40.
Shaw VE, Spana S, Ashkan K, et al. Neuroprotection of midbrain dopaminergic cells in MPTP-treated mice after near-infrared light treatment. J Comp Neurol. 2010;518(1):25-40.
Shaw, V. E., Spana, S., Ashkan, K., Benabid, A. L., Stone, J., Baker, G. E., & Mitrofanis, J. (2010). Neuroprotection of midbrain dopaminergic cells in MPTP-treated mice after near-infrared light treatment. The Journal of Comparative Neurology, 518(1), 25-40. https://doi.org/10.1002/cne.22207
Shaw VE, et al. Neuroprotection of Midbrain Dopaminergic Cells in MPTP-treated Mice After Near-infrared Light Treatment. J Comp Neurol. 2010 Jan 1;518(1):25-40. PubMed PMID: 19882716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotection of midbrain dopaminergic cells in MPTP-treated mice after near-infrared light treatment. AU - Shaw,Victoria E, AU - Spana,Sharon, AU - Ashkan,Keyoumars, AU - Benabid,Alim-Louis, AU - Stone,Jonathan, AU - Baker,Gary E, AU - Mitrofanis,John, PY - 2009/11/3/entrez PY - 2009/11/3/pubmed PY - 2010/1/12/medline SP - 25 EP - 40 JF - The Journal of comparative neurology JO - J Comp Neurol VL - 518 IS - 1 N2 - This study explores whether near-infrared (NIr) light treatment neuroprotects dopaminergic cells in the substantia nigra pars compacta (SNc) and the zona incerta-hypothalamus (ZI-Hyp) from degeneration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. BALB/c albino mice were divided into four groups: 1) Saline, 2) Saline-NIr, 3) MPTP, 4) MPTP-NIr. The injections were intraperitoneal and they were followed immediately by NIr light treatment (or not). Two doses of MPTP, mild (50 mg/kg) and strong (100 mg/kg), were used. Mice were perfused transcardially with aldehyde fixative 6 days after their MPTP treatment. Brains were processed for tyrosine hydroxylase (TH) immunochemistry. The number of TH(+) cells was estimated using the optical fractionator method. Our major finding was that in the SNc there were significantly more dopaminergic cells in the MPTP-NIr compared to the MPTP group (35%-45%). By contrast, in the ZI-Hyp there was no significant difference in the numbers of cells in these two groups. In addition, our results indicated that survival in the two regions after MPTP insult was dose-dependent. In the stronger MPTP regime, the magnitude of loss was similar in the two regions (approximately 60%), while in the milder regime cell loss was greater in the SNc (45%) than ZI-Hyp (approximately 30%). In summary, our results indicate that NIr light treatment offers neuroprotection against MPTP toxicity for dopaminergic cells in the SNc, but not in the ZI-Hyp. SN - 1096-9861 UR - https://www.unboundmedicine.com/medline/citation/19882716/Neuroprotection_of_midbrain_dopaminergic_cells_in_MPTP_treated_mice_after_near_infrared_light_treatment_ L2 - https://doi.org/10.1002/cne.22207 DB - PRIME DP - Unbound Medicine ER -