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Multi-immunogenic outer membrane vesicles derived from an MsbB-deficient Salmonella enterica serovar typhimurium mutant.
J Microbiol Biotechnol. 2009 Oct; 19(10):1271-9.JM

Abstract

To develop low endotoxic and multi-immunogenic outer membrane vesicles (OMVs), a deletion mutant of the msbB gene in Salmonella enterica serovar Typhimurium (S. Typhimurium) was used as a source of low endotoxic OMV, and an expression vector of the canine parvovirus (CPV) VP2 epitope fused to the bacterial OmpA protein was constructed and transformed into the Salmonella deltaAmsbB mutant. In a lethality test, BALB/c mice injected intraperitoneally with the Salmonella deltaAmsbB mutant survived for 7 days, whereas mice injected intraperitoneally with the wild type survived for 3 days. Moreover, all mice inoculated orally with the deltaAmsbB mutant survived for 30 days, but 80% of mice inoculated orally with the wild type survived. The OmpA::CPV VP2 epitope fusion protein was expressed successfully and associated with the outer membrane and OMV fractions from the mutant S. Typhimurium transformed with the fusion protein-expressing vector. In immunogenicity tests, sera obtained from the mice immunized with either the Salmonella msbB mutant or its OMVs containing the OmpA::CPV VP2 epitope showed bactericidal activities against wild-type S. Typhimurium and contained specific antibodies to the CPV VP2 epitope. In the hemagglutination inhibition (HI) assay as a measurement of CPV-neutralizing activity in the immune sera, there was an 8-fold increase of HI titer in the OMV-immunized group compared with the control. These results suggested that the CPV-neutralizing antibody response was raised by immunization with OMV containing the OmpA::CPV VP2 epitope, as well as the protective immune response against S. Typhimurium in BALB/c mice.

Authors+Show Affiliations

The National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Cheongwon, Chungbuk 363-883, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19884791

Citation

Lee, Sang-Rae, et al. "Multi-immunogenic Outer Membrane Vesicles Derived From an MsbB-deficient Salmonella Enterica Serovar Typhimurium Mutant." Journal of Microbiology and Biotechnology, vol. 19, no. 10, 2009, pp. 1271-9.
Lee SR, Kim SH, Jeong KJ, et al. Multi-immunogenic outer membrane vesicles derived from an MsbB-deficient Salmonella enterica serovar typhimurium mutant. J Microbiol Biotechnol. 2009;19(10):1271-9.
Lee, S. R., Kim, S. H., Jeong, K. J., Kim, K. S., Kim, Y. H., Kim, S. J., Kim, E., Kim, J. W., & Chang, K. T. (2009). Multi-immunogenic outer membrane vesicles derived from an MsbB-deficient Salmonella enterica serovar typhimurium mutant. Journal of Microbiology and Biotechnology, 19(10), 1271-9.
Lee SR, et al. Multi-immunogenic Outer Membrane Vesicles Derived From an MsbB-deficient Salmonella Enterica Serovar Typhimurium Mutant. J Microbiol Biotechnol. 2009;19(10):1271-9. PubMed PMID: 19884791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multi-immunogenic outer membrane vesicles derived from an MsbB-deficient Salmonella enterica serovar typhimurium mutant. AU - Lee,Sang-Rae, AU - Kim,Sang-Hyun, AU - Jeong,Kang-Jin, AU - Kim,Keun-Su, AU - Kim,Young-Hyun, AU - Kim,Sung-Jin, AU - Kim,Ekyune, AU - Kim,Jung-Woo, AU - Chang,Kyu-Tae, PY - 2009/11/4/entrez PY - 2009/11/4/pubmed PY - 2010/2/2/medline SP - 1271 EP - 9 JF - Journal of microbiology and biotechnology JO - J Microbiol Biotechnol VL - 19 IS - 10 N2 - To develop low endotoxic and multi-immunogenic outer membrane vesicles (OMVs), a deletion mutant of the msbB gene in Salmonella enterica serovar Typhimurium (S. Typhimurium) was used as a source of low endotoxic OMV, and an expression vector of the canine parvovirus (CPV) VP2 epitope fused to the bacterial OmpA protein was constructed and transformed into the Salmonella deltaAmsbB mutant. In a lethality test, BALB/c mice injected intraperitoneally with the Salmonella deltaAmsbB mutant survived for 7 days, whereas mice injected intraperitoneally with the wild type survived for 3 days. Moreover, all mice inoculated orally with the deltaAmsbB mutant survived for 30 days, but 80% of mice inoculated orally with the wild type survived. The OmpA::CPV VP2 epitope fusion protein was expressed successfully and associated with the outer membrane and OMV fractions from the mutant S. Typhimurium transformed with the fusion protein-expressing vector. In immunogenicity tests, sera obtained from the mice immunized with either the Salmonella msbB mutant or its OMVs containing the OmpA::CPV VP2 epitope showed bactericidal activities against wild-type S. Typhimurium and contained specific antibodies to the CPV VP2 epitope. In the hemagglutination inhibition (HI) assay as a measurement of CPV-neutralizing activity in the immune sera, there was an 8-fold increase of HI titer in the OMV-immunized group compared with the control. These results suggested that the CPV-neutralizing antibody response was raised by immunization with OMV containing the OmpA::CPV VP2 epitope, as well as the protective immune response against S. Typhimurium in BALB/c mice. SN - 1017-7825 UR - https://www.unboundmedicine.com/medline/citation/19884791/Multi_immunogenic_outer_membrane_vesicles_derived_from_an_MsbB_deficient_Salmonella_enterica_serovar_typhimurium_mutant_ L2 - https://medlineplus.gov/salmonellainfections.html DB - PRIME DP - Unbound Medicine ER -