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Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy.
Mol Nutr Food Res 2010; 54(3):373-80MN

Abstract

Kiwifruit has become a frequent cause of fruit allergy in the recent years. The molecular basis of type I hypersensitivity to kiwifruit is attributed to 11 IUIS allergens, with Act d 1, Act d 2 and Act d 5 characterized in extenso. Evaluation of the allergenic properties of Act d 4, a cysteine proteinase inhibitor from green kiwifruit (Actinidia deliciosa) was performed in this study. Identity of the purified glycoprotein was determined by Edman degradation and by mass fingerprint whereby more than 90% of the primary structure of the mature kiwifruit cystatin was confirmed. Using MALDI TOF analysis, molecular masses of 10902.5 and 11055.2 Da were detected for Act d 4, respectively. Positive skin prick reactivity with Act d 4 was induced in three kiwifruit allergic patients, as well as the upregulation of CD63 and CD203c molecules in the basophile activation assay. The IgE reactivity was detected in dot blot analysis while Western blot analysis was negative using sera from six kiwifruit patients, suggesting the presence of conformational IgE epitopes on the Act d 4 molecule. As activator of effector cells in type I hypersensitivity Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Chemistry, Belgrade, Serbia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19885843

Citation

Popovic, Milica M., et al. "Cysteine Proteinase Inhibitor Act D 4 Is a Functional Allergen Contributing to the Clinical Symptoms of Kiwifruit Allergy." Molecular Nutrition & Food Research, vol. 54, no. 3, 2010, pp. 373-80.
Popovic MM, Milovanovic M, Burazer L, et al. Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy. Mol Nutr Food Res. 2010;54(3):373-80.
Popovic, M. M., Milovanovic, M., Burazer, L., Vuckovic, O., Hoffmann-Sommergruber, K., Knulst, A. C., ... Gavrovic-Jankulovic, M. (2010). Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy. Molecular Nutrition & Food Research, 54(3), pp. 373-80. doi:10.1002/mnfr.200900035.
Popovic MM, et al. Cysteine Proteinase Inhibitor Act D 4 Is a Functional Allergen Contributing to the Clinical Symptoms of Kiwifruit Allergy. Mol Nutr Food Res. 2010;54(3):373-80. PubMed PMID: 19885843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy. AU - Popovic,Milica M, AU - Milovanovic,Mina, AU - Burazer,Lidija, AU - Vuckovic,Olga, AU - Hoffmann-Sommergruber,Karin, AU - Knulst,Andre C, AU - Lindner,Buko, AU - Petersen,Arnd, AU - Jankov,Ratko, AU - Gavrovic-Jankulovic,Marija, PY - 2009/11/4/entrez PY - 2009/11/4/pubmed PY - 2010/7/9/medline SP - 373 EP - 80 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 54 IS - 3 N2 - Kiwifruit has become a frequent cause of fruit allergy in the recent years. The molecular basis of type I hypersensitivity to kiwifruit is attributed to 11 IUIS allergens, with Act d 1, Act d 2 and Act d 5 characterized in extenso. Evaluation of the allergenic properties of Act d 4, a cysteine proteinase inhibitor from green kiwifruit (Actinidia deliciosa) was performed in this study. Identity of the purified glycoprotein was determined by Edman degradation and by mass fingerprint whereby more than 90% of the primary structure of the mature kiwifruit cystatin was confirmed. Using MALDI TOF analysis, molecular masses of 10902.5 and 11055.2 Da were detected for Act d 4, respectively. Positive skin prick reactivity with Act d 4 was induced in three kiwifruit allergic patients, as well as the upregulation of CD63 and CD203c molecules in the basophile activation assay. The IgE reactivity was detected in dot blot analysis while Western blot analysis was negative using sera from six kiwifruit patients, suggesting the presence of conformational IgE epitopes on the Act d 4 molecule. As activator of effector cells in type I hypersensitivity Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/19885843/Cysteine_proteinase_inhibitor_Act_d_4_is_a_functional_allergen_contributing_to_the_clinical_symptoms_of_kiwifruit_allergy_ L2 - https://doi.org/10.1002/mnfr.200900035 DB - PRIME DP - Unbound Medicine ER -