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Trigona laeviceps propolis from Thailand: antimicrobial, antiproliferative and cytotoxic activities.
Am J Chin Med 2009; 37(5):855-65AJ

Abstract

Propolis is one of the natural bee products which has long been used as a crude preventative and prophylactic medicine, and has been reported to possess antibacterial, antiviral, anti-inflammatory, antioxidative and anticancer properties. Propolis of the stingless bee, Trigona laeviceps, was extracted by water or methanol at 35% (w/v) yielding a crude water or a methanolic extract at 60 and 80 mg/ml, respectively, which is 17.1 and 22.9% (w/w) of the total propolis, respectively. The antimicrobial activity of both crude extracts was assayed on four selected pathogenic microbes by using the agar well diffusion method. The results suggested that both water and methanolic crude extracts have some antimicrobial activities, water extract has greater antimicrobial activity than methanolic extract. The relative order of sensitivity of the four microbes were, however, the same between the two extracts from the most to least sensitive, S. aureus > E. coli >> C. albicans >>> A. niger, with indeed no observed growth inhibition of A. niger at all. Antiproliferative and cytotoxic affects were tested on the colon carcinoma cell line, SW620, using the three parameters: (1) MTT assay; (2) cell morphology; and (3) the fragmentation of genomic DNA. The water extract of propolis showed a higher antiproliferative activity than that of methanolic extract to SW620 cells, additionally both appeared to cause cell death by necrosis.

Authors+Show Affiliations

Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19885946

Citation

Umthong, Supawadee, et al. "Trigona Laeviceps Propolis From Thailand: Antimicrobial, Antiproliferative and Cytotoxic Activities." The American Journal of Chinese Medicine, vol. 37, no. 5, 2009, pp. 855-65.
Umthong S, Puthong S, Chanchao C. Trigona laeviceps propolis from Thailand: antimicrobial, antiproliferative and cytotoxic activities. Am J Chin Med. 2009;37(5):855-65.
Umthong, S., Puthong, S., & Chanchao, C. (2009). Trigona laeviceps propolis from Thailand: antimicrobial, antiproliferative and cytotoxic activities. The American Journal of Chinese Medicine, 37(5), pp. 855-65.
Umthong S, Puthong S, Chanchao C. Trigona Laeviceps Propolis From Thailand: Antimicrobial, Antiproliferative and Cytotoxic Activities. Am J Chin Med. 2009;37(5):855-65. PubMed PMID: 19885946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trigona laeviceps propolis from Thailand: antimicrobial, antiproliferative and cytotoxic activities. AU - Umthong,Supawadee, AU - Puthong,Songchan, AU - Chanchao,Chanpen, PY - 2009/11/4/entrez PY - 2009/11/4/pubmed PY - 2010/1/12/medline SP - 855 EP - 65 JF - The American journal of Chinese medicine JO - Am. J. Chin. Med. VL - 37 IS - 5 N2 - Propolis is one of the natural bee products which has long been used as a crude preventative and prophylactic medicine, and has been reported to possess antibacterial, antiviral, anti-inflammatory, antioxidative and anticancer properties. Propolis of the stingless bee, Trigona laeviceps, was extracted by water or methanol at 35% (w/v) yielding a crude water or a methanolic extract at 60 and 80 mg/ml, respectively, which is 17.1 and 22.9% (w/w) of the total propolis, respectively. The antimicrobial activity of both crude extracts was assayed on four selected pathogenic microbes by using the agar well diffusion method. The results suggested that both water and methanolic crude extracts have some antimicrobial activities, water extract has greater antimicrobial activity than methanolic extract. The relative order of sensitivity of the four microbes were, however, the same between the two extracts from the most to least sensitive, S. aureus > E. coli >> C. albicans >>> A. niger, with indeed no observed growth inhibition of A. niger at all. Antiproliferative and cytotoxic affects were tested on the colon carcinoma cell line, SW620, using the three parameters: (1) MTT assay; (2) cell morphology; and (3) the fragmentation of genomic DNA. The water extract of propolis showed a higher antiproliferative activity than that of methanolic extract to SW620 cells, additionally both appeared to cause cell death by necrosis. SN - 0192-415X UR - https://www.unboundmedicine.com/medline/citation/19885946/Trigona_laeviceps_propolis_from_Thailand:_antimicrobial_antiproliferative_and_cytotoxic_activities_ L2 - https://www.worldscientific.com/doi/full/10.1142/S0192415X09007338?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -