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Structural determination and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps sinensis.
Am J Chin Med. 2009; 37(5):977-89.AJ

Abstract

A water-soluble polysaccharide named CPS1 had been isolated from C. sinensis mycelium by hot water extraction, ethanol precipitation, anion-exchange, and gel-permeation chromatography. UV spectra, FTIR spectra, partial acid hydrolysis, PMP precolumn derivation, periodate oxidation and Smith degradation studies were conducted to elucidate its structure. The results indicated that CPS1 was a glucomannogalactan with the monosaccharide composition of glucose: mannose: galactose = 2.8: 2.9: 1. The total carbohydrate content of CPS1 was 99.0%. The weight-average molecular weight was 8.1 x 10(3) Da. The results predicted (1-->2) and (1-->4)-linkage of mannose, (1-->3)-linkage of galactose, (1-->) and (1-->3, 6)-linkage of glucose composed the backbone of CPS1. CPS1 was also evaluated for its antioxidant activity in vitro, including scavenging effects on the hydroxyl radicals, the reducing power, Fe(2+)-chelating activity, scavenging effect on superoxide radicals, as well as the inhibition of hydrogen peroxide induced haemolysis. CPS1 showed a high antioxidant effect, especially scavenging effect of hydroxyl radicals, the reducing power and Fe(2+)-chelating activity. The results provide scientific support for the antioxidant activity and indicated a connection between antioxidant activity and reparation of renal failure.

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Authors+Show Affiliations

Wang Y
Department of Microbiology and Biochemistry, School of Life Science and Technology, China Pharmaceutical University, Jiangsu, China. wangying19830511@hotmail.com
Wang M
No affiliation info available
Ling Y
No affiliation info available
Fan W
No affiliation info available
Wang Y
No affiliation info available
Yin H
No affiliation info available

MeSH

AnimalsAntioxidantsChelating AgentsChromatography, High Pressure LiquidCordycepsDose-Response Relationship, DrugFree Radical ScavengersFruiting Bodies, FungalGalactoseGlucoseHemolysisHydrogen PeroxideIronMannoseMiceMolecular WeightOxidation-ReductionPolysaccharides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19885957

Citation

Wang, Ying, et al. "Structural Determination and Antioxidant Activity of a Polysaccharide From the Fruiting Bodies of Cultured Cordyceps Sinensis." The American Journal of Chinese Medicine, vol. 37, no. 5, 2009, pp. 977-89.
Wang Y, Wang M, Ling Y, et al. Structural determination and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps sinensis. Am J Chin Med. 2009;37(5):977-89.
Wang, Y., Wang, M., Ling, Y., Fan, W., Wang, Y., & Yin, H. (2009). Structural determination and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps sinensis. The American Journal of Chinese Medicine, 37(5), 977-89.
Wang Y, et al. Structural Determination and Antioxidant Activity of a Polysaccharide From the Fruiting Bodies of Cultured Cordyceps Sinensis. Am J Chin Med. 2009;37(5):977-89. PubMed PMID: 19885957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural determination and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps sinensis. AU - Wang,Ying, AU - Wang,Min, AU - Ling,Yun, AU - Fan,Weiqiang, AU - Wang,Yufeng, AU - Yin,Hongping, PY - 2009/11/4/entrez PY - 2009/11/4/pubmed PY - 2010/1/12/medline SP - 977 EP - 89 JF - The American journal of Chinese medicine JO - Am J Chin Med VL - 37 IS - 5 N2 - A water-soluble polysaccharide named CPS1 had been isolated from C. sinensis mycelium by hot water extraction, ethanol precipitation, anion-exchange, and gel-permeation chromatography. UV spectra, FTIR spectra, partial acid hydrolysis, PMP precolumn derivation, periodate oxidation and Smith degradation studies were conducted to elucidate its structure. The results indicated that CPS1 was a glucomannogalactan with the monosaccharide composition of glucose: mannose: galactose = 2.8: 2.9: 1. The total carbohydrate content of CPS1 was 99.0%. The weight-average molecular weight was 8.1 x 10(3) Da. The results predicted (1-->2) and (1-->4)-linkage of mannose, (1-->3)-linkage of galactose, (1-->) and (1-->3, 6)-linkage of glucose composed the backbone of CPS1. CPS1 was also evaluated for its antioxidant activity in vitro, including scavenging effects on the hydroxyl radicals, the reducing power, Fe(2+)-chelating activity, scavenging effect on superoxide radicals, as well as the inhibition of hydrogen peroxide induced haemolysis. CPS1 showed a high antioxidant effect, especially scavenging effect of hydroxyl radicals, the reducing power and Fe(2+)-chelating activity. The results provide scientific support for the antioxidant activity and indicated a connection between antioxidant activity and reparation of renal failure. SN - 0192-415X UR - https://www.unboundmedicine.com/medline/citation/19885957/Structural_determination_and_antioxidant_activity_of_a_polysaccharide_from_the_fruiting_bodies_of_cultured_Cordyceps_sinensis_ L2 - https://www.worldscientific.com/doi/10.1142/S0192415X09007387?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -