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[Expression CD27 on T and B lymphocytes in hypertrophied adenoids at children with otitis media with effusion].
Otolaryngol Pol. 2009 May-Jun; 63(3):264-70.OP

Abstract

INTRODUCTION

Adenoid has particular meaning to develop of immunological response to inflammations in upper respiratory inclusive middle ear. The mining of antigen CD27 on lymphocytes T and B in creation of memory cells is still unclear.

AIM

CD27 on lymphocytes T and B has a crucial role in development of immune response against inflammatory state. Aim of this study was evaluation functions of lymhocytes with expression CD27 in hypertrophied adenoid in children with otitis media with effusion.

MATERIAL AND METHODS

[corrected] We tested 24 children in examine group with hypertrophied adenoid and otitis media with effusion (OME), and 29 children in comparative group only with hypertrophied adenoid (HA). We also divided both groups into two groups, above 5 and over 5 years old. We made the research by flow cytometry method. We used anty-CD19, anty-CD3, anty-CD5 and anty-CD27 monoclonal antibodies to examinations.

RESULTS

We showed the lowest percentage lymphocytes B CD5+ with expression of CD27 in subgroup older children with otitis media with effusion (O.M.E. 22.43 +/- 2.66%) in compartative to younger children in the same group (15.96 +/- 5.31%; p < 0.005) and to older comparative subgroup (H.A. 15.50 +/- 8.03; p < 0.001). Percentages of B CD5 cells with expression of CD27 was statistical lower in examinated group (7.25 +/- 3.81) than in comparative group (H.A. 16.26 +/- 5.82; p < 0.04). In group with hypertrophied adenoid showed higher percentages of CD5-CD27+CD19+ cells both in younger and older supgroup than in equivalent supgroups with O.M.E.

CONCLUSIONS

Iteraction of CD27-CD70 regulates many functions of T and B lymphocytes. It seems that disorders humoral immunologucal answer in hypertrophied adenoids are supported to develop of otitis media with effusion.

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Authors+Show Affiliations

Ratomski K
Zakład Laboratoryjnej Diagnostyki Pediatrycznej Uniwersytetu Medycznego w Białymstoku. karol.cam@interia.pl
Zelazowska-Rutkowska B
No affiliation info available
Wysocka J
No affiliation info available
Skotnicka B
No affiliation info available
Kasprzycka E
No affiliation info available
Hassmann-Poznańska E
No affiliation info available

MeSH

AdenoidsAntigens, CD19B-LymphocytesBiomarkersCD3 ComplexCD4-Positive T-LymphocytesCD5 AntigensChildChild, PreschoolFemaleFlow CytometryHumansHypertrophyOtitis Media with EffusionSeverity of Illness IndexStatistics, NonparametricT-LymphocytesTonsillectomyTumor Necrosis Factor Receptor Superfamily, Member 7

Pub Type(s)

Journal Article

Language

pol

PubMed ID

19886534

Citation

Ratomski, Karol, et al. "[Expression CD27 On T and B Lymphocytes in Hypertrophied Adenoids at Children With Otitis Media With Effusion]." Otolaryngologia Polska = the Polish Otolaryngology, vol. 63, no. 3, 2009, pp. 264-70.
Ratomski K, Zelazowska-Rutkowska B, Wysocka J, et al. [Expression CD27 on T and B lymphocytes in hypertrophied adenoids at children with otitis media with effusion]. Otolaryngol Pol. 2009;63(3):264-70.
Ratomski, K., Zelazowska-Rutkowska, B., Wysocka, J., Skotnicka, B., Kasprzycka, E., & Hassmann-Poznańska, E. (2009). [Expression CD27 on T and B lymphocytes in hypertrophied adenoids at children with otitis media with effusion]. Otolaryngologia Polska = the Polish Otolaryngology, 63(3), 264-70. https://doi.org/10.1016/S0030-6657(09)70120-9
Ratomski K, et al. [Expression CD27 On T and B Lymphocytes in Hypertrophied Adenoids at Children With Otitis Media With Effusion]. Otolaryngol Pol. 2009 May-Jun;63(3):264-70. PubMed PMID: 19886534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression CD27 on T and B lymphocytes in hypertrophied adenoids at children with otitis media with effusion]. AU - Ratomski,Karol, AU - Zelazowska-Rutkowska,Beata, AU - Wysocka,Jolanta, AU - Skotnicka,Bozena, AU - Kasprzycka,Edwina, AU - Hassmann-Poznańska,Elzbieta, PY - 2009/11/6/entrez PY - 2009/11/6/pubmed PY - 2009/12/16/medline SP - 264 EP - 70 JF - Otolaryngologia polska = The Polish otolaryngology JO - Otolaryngol Pol VL - 63 IS - 3 N2 - INTRODUCTION: Adenoid has particular meaning to develop of immunological response to inflammations in upper respiratory inclusive middle ear. The mining of antigen CD27 on lymphocytes T and B in creation of memory cells is still unclear. AIM: CD27 on lymphocytes T and B has a crucial role in development of immune response against inflammatory state. Aim of this study was evaluation functions of lymhocytes with expression CD27 in hypertrophied adenoid in children with otitis media with effusion. MATERIAL AND METHODS: [corrected] We tested 24 children in examine group with hypertrophied adenoid and otitis media with effusion (OME), and 29 children in comparative group only with hypertrophied adenoid (HA). We also divided both groups into two groups, above 5 and over 5 years old. We made the research by flow cytometry method. We used anty-CD19, anty-CD3, anty-CD5 and anty-CD27 monoclonal antibodies to examinations. RESULTS: We showed the lowest percentage lymphocytes B CD5+ with expression of CD27 in subgroup older children with otitis media with effusion (O.M.E. 22.43 +/- 2.66%) in compartative to younger children in the same group (15.96 +/- 5.31%; p < 0.005) and to older comparative subgroup (H.A. 15.50 +/- 8.03; p < 0.001). Percentages of B CD5 cells with expression of CD27 was statistical lower in examinated group (7.25 +/- 3.81) than in comparative group (H.A. 16.26 +/- 5.82; p < 0.04). In group with hypertrophied adenoid showed higher percentages of CD5-CD27+CD19+ cells both in younger and older supgroup than in equivalent supgroups with O.M.E. CONCLUSIONS: Iteraction of CD27-CD70 regulates many functions of T and B lymphocytes. It seems that disorders humoral immunologucal answer in hypertrophied adenoids are supported to develop of otitis media with effusion. SN - 0030-6657 UR - https://www.unboundmedicine.com/medline/citation/19886534/[Expression_CD27_on_T_and_B_lymphocytes_in_hypertrophied_adenoids_at_children_with_otitis_media_with_effusion]_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0030-6657(09)70120-9 DB - PRIME DP - Unbound Medicine ER -