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Augmentation of expression of immunocytes' functions by seed extract of Ziziphus mauritiana (Lamk.).
J Ethnopharmacol. 2010 Feb 03; 127(2):341-5.JE

Abstract

AIM OF THE STUDY

The present study was carried out to evaluate the immunomodulatory potential of Ziziphus mauritiana (Lamk.) seed extract to ascertain the folkloric claim as immunomodulator.

MATERIALS AND METHODS

The aqueous-ethanolic seed extract (100-400 mg kg(-1)) of Z. mauritiana was investigated for immunomodulatory potential in mice. The extract was standardized with HPLC using betulinic acid as a marker. Functions of various immunocytes in the form of humoral (development of anti-SRBC (sheep red blood cells) antibody titers) and cell-mediated immune response (delayed type hypersensitivity, nitroblue tetrazolium reduction, inducible nitric oxide synthase activity and bactericidal activity) was studied in SRBC immunized mice. The cytokine, IFN-gamma (interferon-gamma) and IL-4 (interleukin-4) secretion was also measured quantitatively by ELISA as the expression of functions of Th-1 and Th-2 respectively. Levamisole (2.5 mg kg(-1)) was used as standard drug.

RESULTS

The seed extract demonstrated significant (P<0.05-0.001) up-regulation of cell-mediated, humoral immune response and Th-1 mediated cytokine IFN-gamma and decline in Th-2 mediated cytokine IL-4. At higher dose of extract the results were comparable to that of the levamisole.

CONCLUSION

The immunostimulatory potential of this seed extract is likely to be mediated through its effect on macrophage function and Th-1 mediated immunity confirming the folkloric use of this plant.

Authors+Show Affiliations

Immunology and Immunotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147 002, Punjab, India.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19887103

Citation

Mishra, Tulika, and Aruna Bhatia. "Augmentation of Expression of Immunocytes' Functions By Seed Extract of Ziziphus Mauritiana (Lamk.)." Journal of Ethnopharmacology, vol. 127, no. 2, 2010, pp. 341-5.
Mishra T, Bhatia A. Augmentation of expression of immunocytes' functions by seed extract of Ziziphus mauritiana (Lamk.). J Ethnopharmacol. 2010;127(2):341-5.
Mishra, T., & Bhatia, A. (2010). Augmentation of expression of immunocytes' functions by seed extract of Ziziphus mauritiana (Lamk.). Journal of Ethnopharmacology, 127(2), 341-5. https://doi.org/10.1016/j.jep.2009.10.033
Mishra T, Bhatia A. Augmentation of Expression of Immunocytes' Functions By Seed Extract of Ziziphus Mauritiana (Lamk.). J Ethnopharmacol. 2010 Feb 3;127(2):341-5. PubMed PMID: 19887103.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Augmentation of expression of immunocytes' functions by seed extract of Ziziphus mauritiana (Lamk.). AU - Mishra,Tulika, AU - Bhatia,Aruna, Y1 - 2009/11/01/ PY - 2008/03/16/received PY - 2009/10/15/revised PY - 2009/10/27/accepted PY - 2009/11/6/entrez PY - 2009/11/6/pubmed PY - 2010/9/9/medline SP - 341 EP - 5 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 127 IS - 2 N2 - AIM OF THE STUDY: The present study was carried out to evaluate the immunomodulatory potential of Ziziphus mauritiana (Lamk.) seed extract to ascertain the folkloric claim as immunomodulator. MATERIALS AND METHODS: The aqueous-ethanolic seed extract (100-400 mg kg(-1)) of Z. mauritiana was investigated for immunomodulatory potential in mice. The extract was standardized with HPLC using betulinic acid as a marker. Functions of various immunocytes in the form of humoral (development of anti-SRBC (sheep red blood cells) antibody titers) and cell-mediated immune response (delayed type hypersensitivity, nitroblue tetrazolium reduction, inducible nitric oxide synthase activity and bactericidal activity) was studied in SRBC immunized mice. The cytokine, IFN-gamma (interferon-gamma) and IL-4 (interleukin-4) secretion was also measured quantitatively by ELISA as the expression of functions of Th-1 and Th-2 respectively. Levamisole (2.5 mg kg(-1)) was used as standard drug. RESULTS: The seed extract demonstrated significant (P<0.05-0.001) up-regulation of cell-mediated, humoral immune response and Th-1 mediated cytokine IFN-gamma and decline in Th-2 mediated cytokine IL-4. At higher dose of extract the results were comparable to that of the levamisole. CONCLUSION: The immunostimulatory potential of this seed extract is likely to be mediated through its effect on macrophage function and Th-1 mediated immunity confirming the folkloric use of this plant. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19887103/Augmentation_of_expression_of_immunocytes'_functions_by_seed_extract_of_Ziziphus_mauritiana__Lamk___ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00690-4 DB - PRIME DP - Unbound Medicine ER -