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Changes in faecal microbiota of infants with cow's milk protein allergy--a Spanish prospective case-control 6-month follow-up study.
Pediatr Allergy Immunol. 2010 Mar; 21(2 Pt 2):e394-400.PA

Abstract

Major differences have been found in gut microbiota between healthy and allergic children, and a possible association between allergy and altered microbiota patterns have been postulated. The main object of the study was to compare the faecal microbiota between healthy and cow's milk protein allergy (CMPA) infants at the baseline immediately after the diagnosis, and to evaluate the changes in the faecal microbiota after 6 months of treatment of CMPA infants fed on extensively cow's milk protein hydrolyzed formulae without pre- or probiotics, compared with healthy children fed on standard milk formulae. The population comprised 92 infants aged 2-12 months who were non-allergic (n=46) or diagnosed with IgE-mediated CMPA (n=46). At baseline and at 6 months, faecal samples were collected into sterile plastic tubes, immediately placed into anaerobic jars and processed within 2 h of their collection. Weighed faeces samples were diluted from 10(-1) to 10(-7) and cultured in selective media for total count of aerobes, anaerobes, enterobacteria, bifidobacteria, lactobacilli, clostridia and yeasts. Samples from non-allergic and allergic infants were compared at baseline and at 6 months using appropriate statistical tests, considering p<0.05 to be significant. In comparison with healthy infants, CMPA infants had higher total bacteria and anaerobic counts and a lower yeast count at baseline, finding no difference in the proportions of each bacterial group between groups. After 6 months, CMPA infants showed higher anaerobic and lactobacilli counts, a higher proportion of lactobacilli, a lower count and proportion of bifidobacteria, and lower proportions of enterobacteria and yeasts. Comparison of faecal samples from CMPA infants between baseline and at 6 months showed an increase in count and proportion of lactobacilli and a decrease in counts and proportions of enterobacteria and bifidobacteria. Differences in the composition of gut microbiota between CMPA and healthy infants may influence in the development of or protection from this allergy.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology II, University of Granada, Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19889194

Citation

Thompson-Chagoyan, Oscar C., et al. "Changes in Faecal Microbiota of Infants With Cow's Milk Protein Allergy--a Spanish Prospective Case-control 6-month Follow-up Study." Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology, vol. 21, no. 2 Pt 2, 2010, pp. e394-400.
Thompson-Chagoyan OC, Vieites JM, Maldonado J, et al. Changes in faecal microbiota of infants with cow's milk protein allergy--a Spanish prospective case-control 6-month follow-up study. Pediatr Allergy Immunol. 2010;21(2 Pt 2):e394-400.
Thompson-Chagoyan, O. C., Vieites, J. M., Maldonado, J., Edwards, C., & Gil, A. (2010). Changes in faecal microbiota of infants with cow's milk protein allergy--a Spanish prospective case-control 6-month follow-up study. Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology, 21(2 Pt 2), e394-400. https://doi.org/10.1111/j.1399-3038.2009.00961.x
Thompson-Chagoyan OC, et al. Changes in Faecal Microbiota of Infants With Cow's Milk Protein Allergy--a Spanish Prospective Case-control 6-month Follow-up Study. Pediatr Allergy Immunol. 2010;21(2 Pt 2):e394-400. PubMed PMID: 19889194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in faecal microbiota of infants with cow's milk protein allergy--a Spanish prospective case-control 6-month follow-up study. AU - Thompson-Chagoyan,Oscar C, AU - Vieites,Jose M, AU - Maldonado,Jose, AU - Edwards,Christine, AU - Gil,Angel, Y1 - 2009/11/02/ PY - 2009/11/6/entrez PY - 2009/11/6/pubmed PY - 2010/8/24/medline SP - e394 EP - 400 JF - Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology JO - Pediatr Allergy Immunol VL - 21 IS - 2 Pt 2 N2 - Major differences have been found in gut microbiota between healthy and allergic children, and a possible association between allergy and altered microbiota patterns have been postulated. The main object of the study was to compare the faecal microbiota between healthy and cow's milk protein allergy (CMPA) infants at the baseline immediately after the diagnosis, and to evaluate the changes in the faecal microbiota after 6 months of treatment of CMPA infants fed on extensively cow's milk protein hydrolyzed formulae without pre- or probiotics, compared with healthy children fed on standard milk formulae. The population comprised 92 infants aged 2-12 months who were non-allergic (n=46) or diagnosed with IgE-mediated CMPA (n=46). At baseline and at 6 months, faecal samples were collected into sterile plastic tubes, immediately placed into anaerobic jars and processed within 2 h of their collection. Weighed faeces samples were diluted from 10(-1) to 10(-7) and cultured in selective media for total count of aerobes, anaerobes, enterobacteria, bifidobacteria, lactobacilli, clostridia and yeasts. Samples from non-allergic and allergic infants were compared at baseline and at 6 months using appropriate statistical tests, considering p<0.05 to be significant. In comparison with healthy infants, CMPA infants had higher total bacteria and anaerobic counts and a lower yeast count at baseline, finding no difference in the proportions of each bacterial group between groups. After 6 months, CMPA infants showed higher anaerobic and lactobacilli counts, a higher proportion of lactobacilli, a lower count and proportion of bifidobacteria, and lower proportions of enterobacteria and yeasts. Comparison of faecal samples from CMPA infants between baseline and at 6 months showed an increase in count and proportion of lactobacilli and a decrease in counts and proportions of enterobacteria and bifidobacteria. Differences in the composition of gut microbiota between CMPA and healthy infants may influence in the development of or protection from this allergy. SN - 1399-3038 UR - https://www.unboundmedicine.com/medline/citation/19889194/Changes_in_faecal_microbiota_of_infants_with_cow's_milk_protein_allergy__a_Spanish_prospective_case_control_6_month_follow_up_study_ L2 - https://doi.org/10.1111/j.1399-3038.2009.00961.x DB - PRIME DP - Unbound Medicine ER -