Tags

Type your tag names separated by a space and hit enter

Influence of bicarbonate and humic acid on effects of chronic waterborne lead exposure to the fathead minnow (Pimephales promelas).
Aquat Toxicol. 2010 Jan 31; 96(2):135-44.AT

Abstract

Historically, the USEPA has only considered water hardness when establishing acute and chronic water quality criteria (WQC) for lead (Pb) in freshwater. Yet, recent evidence suggests that hardness may not be protective during chronic Pb exposure and that other factors (e.g., dissolved organic carbon (DOC) and alkalinity) influence toxicity. In fact, we have recently shown that Ca(2+) (as CaSO(4)) does not protect against Pb accumulation in fathead minnows (Pimephales promelas) during chronic exposures whereas DOC as humic acid (HA) clearly does. To more clearly define the water chemistry parameters mediating chronic Pb toxicity we carried out 300 d exposures to study the influence of DOC and alkalinity on Pb accumulation and toxicity to fathead minnows at 2 different Pb concentrations (170 and 580 nM (35 and 120 microg/L)). Alkalinity was adjusted by addition of 500 microM NaHCO(3) and DOC by addition of 4 mg/L HA. Fish were collected at 4, 30, 150 and 300 d of exposure to measure growth and Pb accumulation. Breeding assays (21 d) were performed at the end of these exposures to assess reproductive and larval behavioral endpoints. To determine whether effects were acute or chronic, switched breeding exposures were performed in which control breeders were transferred to either high or low Pb conditions and Pb-exposed breeders transferred to tap water without Pb. Mortality and growth effects were observed primarily in the high Pb treatments and within the first 10 d of exposure. Strong protection against Pb accumulation was afforded by increased DOC at both Pb concentrations. Increased alkalinity also appeared to moderately reduce Pb accumulation although not to the level of statistical significance. Tissue distribution of Pb was analyzed at 300 d and was found to accumulate mostly in bone, gill, intestine and kidney. Unexpectedly, high Pb reduced total reproductive output and increased average egg mass in the HCO(3)(-) and DOC treatments but not in the control water (+Pb) treatments. No statistically significant differences in egg hatchability or egg Pb accumulation were observed. Results from switched exposures suggest that embryo Pb accumulation arose from acute exposure to embryos rather than parental transfer. Finally, prey capture assays revealed potential Pb-induced motor/behavioral impairment in 10-d-old F1 larvae exposed to high Pb in all water treatments.

Links

Publisher Full Text

Authors+Show Affiliations

Mager EM
Division of Marine Biology and Fisheries, University of Miami, Rosenstiel School of Marine and Atmospheric Science, 4600 Rickenbacker Causeway, Miami, FL 33149-1098, USA. emager@rsmas.miami.edu
Brix KV
No affiliation info available
Grosell M
No affiliation info available

MeSH

AnimalsBicarbonatesCyprinidaeEnvironmental ExposureFemaleFertilityHumic SubstancesLarvaLeadMaleOvumWaterWater Pollutants, Chemical

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19889467

Citation

Mager, Edward M., et al. "Influence of Bicarbonate and Humic Acid On Effects of Chronic Waterborne Lead Exposure to the Fathead Minnow (Pimephales Promelas)." Aquatic Toxicology (Amsterdam, Netherlands), vol. 96, no. 2, 2010, pp. 135-44.
Mager EM, Brix KV, Grosell M. Influence of bicarbonate and humic acid on effects of chronic waterborne lead exposure to the fathead minnow (Pimephales promelas). Aquat Toxicol. 2010;96(2):135-44.
Mager, E. M., Brix, K. V., & Grosell, M. (2010). Influence of bicarbonate and humic acid on effects of chronic waterborne lead exposure to the fathead minnow (Pimephales promelas). Aquatic Toxicology (Amsterdam, Netherlands), 96(2), 135-44. https://doi.org/10.1016/j.aquatox.2009.10.012
Mager EM, Brix KV, Grosell M. Influence of Bicarbonate and Humic Acid On Effects of Chronic Waterborne Lead Exposure to the Fathead Minnow (Pimephales Promelas). Aquat Toxicol. 2010 Jan 31;96(2):135-44. PubMed PMID: 19889467.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of bicarbonate and humic acid on effects of chronic waterborne lead exposure to the fathead minnow (Pimephales promelas). AU - Mager,Edward M, AU - Brix,Kevin V, AU - Grosell,Martin, Y1 - 2009/11/03/ PY - 2009/07/09/received PY - 2009/10/07/revised PY - 2009/10/08/accepted PY - 2009/11/6/entrez PY - 2009/11/6/pubmed PY - 2010/4/7/medline SP - 135 EP - 44 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 96 IS - 2 N2 - Historically, the USEPA has only considered water hardness when establishing acute and chronic water quality criteria (WQC) for lead (Pb) in freshwater. Yet, recent evidence suggests that hardness may not be protective during chronic Pb exposure and that other factors (e.g., dissolved organic carbon (DOC) and alkalinity) influence toxicity. In fact, we have recently shown that Ca(2+) (as CaSO(4)) does not protect against Pb accumulation in fathead minnows (Pimephales promelas) during chronic exposures whereas DOC as humic acid (HA) clearly does. To more clearly define the water chemistry parameters mediating chronic Pb toxicity we carried out 300 d exposures to study the influence of DOC and alkalinity on Pb accumulation and toxicity to fathead minnows at 2 different Pb concentrations (170 and 580 nM (35 and 120 microg/L)). Alkalinity was adjusted by addition of 500 microM NaHCO(3) and DOC by addition of 4 mg/L HA. Fish were collected at 4, 30, 150 and 300 d of exposure to measure growth and Pb accumulation. Breeding assays (21 d) were performed at the end of these exposures to assess reproductive and larval behavioral endpoints. To determine whether effects were acute or chronic, switched breeding exposures were performed in which control breeders were transferred to either high or low Pb conditions and Pb-exposed breeders transferred to tap water without Pb. Mortality and growth effects were observed primarily in the high Pb treatments and within the first 10 d of exposure. Strong protection against Pb accumulation was afforded by increased DOC at both Pb concentrations. Increased alkalinity also appeared to moderately reduce Pb accumulation although not to the level of statistical significance. Tissue distribution of Pb was analyzed at 300 d and was found to accumulate mostly in bone, gill, intestine and kidney. Unexpectedly, high Pb reduced total reproductive output and increased average egg mass in the HCO(3)(-) and DOC treatments but not in the control water (+Pb) treatments. No statistically significant differences in egg hatchability or egg Pb accumulation were observed. Results from switched exposures suggest that embryo Pb accumulation arose from acute exposure to embryos rather than parental transfer. Finally, prey capture assays revealed potential Pb-induced motor/behavioral impairment in 10-d-old F1 larvae exposed to high Pb in all water treatments. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/19889467/Influence_of_bicarbonate_and_humic_acid_on_effects_of_chronic_waterborne_lead_exposure_to_the_fathead_minnow__Pimephales_promelas__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(09)00351-8 DB - PRIME DP - Unbound Medicine ER -