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Syk expression in peripheral blood leukocytes, CD34+ progenitors, and CD34-derived basophils.
J Leukoc Biol 2010; 87(2):291-300JL

Abstract

In human basophils from different subjects, maximum IgE-mediated histamine release and the level of syk protein expression correlate well. It is not clear when in the basophil's lifetime the set-point for syk expression is reached or how expression levels are determined for a given individual. An examination of syk expression in peripheral blood eosinophils, neutrophils, monocytes, B and T cells, DCs, and NK cells showed that with the exception of T cells, basophils were unique in expressing low levels of syk. No correlations were observed between syk expression in basophils and other types of leukocytes, suggesting a unique mechanism of regulation for basophils. The expression level of syk in CD34+ progenitors was approximately 11-fold higher than in peripheral blood basophils, and it remained at this level during maturation of the cells in IL-3 to a cell with characteristics of peripheral blood basophils. Down-regulation of syk expression in the culture-derived basophils was induced by culturing under conditions of chronic aggregation of FcepsilonRI. Syk was down-regulated to peripheral blood basophil levels in 50% of the cells. Despite the chronic aggregation of FcepsilonRI, the cells retained the same expression of FcepsilonRI, histamine content, and morphological staining of granules as cells not experiencing chronic aggregation. These results suggest that chronic stimulation through FcepsilonRI during basophil maturation might be a mechanism for down-regulating syk expression, while retaining other characteristics associated with mature peripheral blood basophils.

Authors+Show Affiliations

Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19889729

Citation

Ishmael, Susan S., and Donald W. MacGlashan. "Syk Expression in Peripheral Blood Leukocytes, CD34+ Progenitors, and CD34-derived Basophils." Journal of Leukocyte Biology, vol. 87, no. 2, 2010, pp. 291-300.
Ishmael SS, MacGlashan DW. Syk expression in peripheral blood leukocytes, CD34+ progenitors, and CD34-derived basophils. J Leukoc Biol. 2010;87(2):291-300.
Ishmael, S. S., & MacGlashan, D. W. (2010). Syk expression in peripheral blood leukocytes, CD34+ progenitors, and CD34-derived basophils. Journal of Leukocyte Biology, 87(2), pp. 291-300. doi:10.1189/jlb.0509336.
Ishmael SS, MacGlashan DW. Syk Expression in Peripheral Blood Leukocytes, CD34+ Progenitors, and CD34-derived Basophils. J Leukoc Biol. 2010;87(2):291-300. PubMed PMID: 19889729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Syk expression in peripheral blood leukocytes, CD34+ progenitors, and CD34-derived basophils. AU - Ishmael,Susan S, AU - MacGlashan,Donald W,Jr Y1 - 2009/11/04/ PY - 2009/11/6/entrez PY - 2009/11/6/pubmed PY - 2010/2/26/medline SP - 291 EP - 300 JF - Journal of leukocyte biology JO - J. Leukoc. Biol. VL - 87 IS - 2 N2 - In human basophils from different subjects, maximum IgE-mediated histamine release and the level of syk protein expression correlate well. It is not clear when in the basophil's lifetime the set-point for syk expression is reached or how expression levels are determined for a given individual. An examination of syk expression in peripheral blood eosinophils, neutrophils, monocytes, B and T cells, DCs, and NK cells showed that with the exception of T cells, basophils were unique in expressing low levels of syk. No correlations were observed between syk expression in basophils and other types of leukocytes, suggesting a unique mechanism of regulation for basophils. The expression level of syk in CD34+ progenitors was approximately 11-fold higher than in peripheral blood basophils, and it remained at this level during maturation of the cells in IL-3 to a cell with characteristics of peripheral blood basophils. Down-regulation of syk expression in the culture-derived basophils was induced by culturing under conditions of chronic aggregation of FcepsilonRI. Syk was down-regulated to peripheral blood basophil levels in 50% of the cells. Despite the chronic aggregation of FcepsilonRI, the cells retained the same expression of FcepsilonRI, histamine content, and morphological staining of granules as cells not experiencing chronic aggregation. These results suggest that chronic stimulation through FcepsilonRI during basophil maturation might be a mechanism for down-regulating syk expression, while retaining other characteristics associated with mature peripheral blood basophils. SN - 1938-3673 UR - https://www.unboundmedicine.com/medline/citation/19889729/Syk_expression_in_peripheral_blood_leukocytes_CD34+_progenitors_and_CD34_derived_basophils_ L2 - https://doi.org/10.1189/jlb.0509336 DB - PRIME DP - Unbound Medicine ER -