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Evaluation of the genotoxic and antigenotoxic effects after acute and subacute treatments with açai pulp (Euterpe oleracea Mart.) on mice using the erythrocytes micronucleus test and the comet assay.
Mutat Res. 2010 Jan; 695(1-2):22-8.MR

Abstract

Açai, the fruit of a palm native to the Amazonian basin, is widely distributed in northern South America, where it has considerable economic importance. Whereas individual polyphenolics compounds in açai have been extensively evaluated, studies of the intact fruit and its biological properties are lacking. Therefore, the present study was undertaken to investigate the in vivo genotoxicity of açai and its possible antigenotoxicity on doxorubicin (DXR)-induced DNA damage. The açai pulp doses selected were 3.33, 10.0 and 16.67g/kg b.w. administered by gavage alone or prior to DXR (16mg/kg b.w.) administered by intraperitoneal injection. Swiss albino mice were distributed in eight groups for acute treatment with açai pulp (24h) and eight groups for subacute treatment (daily for 14 consecutive days) before euthanasia. The negative control groups were treated in a similar way. The results of chemical analysis suggested the presence of carotenoids, anthocyanins, phenolic, and flavonoids in açai pulp. The endpoints analyzed were micronucleus induction in bone marrow and peripheral blood cells polychromatic erythrocytes, and DNA damage in peripheral blood, liver and kidney cells assessed using the alkaline (pH >13) comet assay. There were no statistically significant differences (p>0.05) between the negative control and the groups treated with the three doses of açai pulp alone in all endpoints analyzed, demonstrating the absence of genotoxic effects. The protective effects of açai pulp were observed in both acute and subacute treatments, when administered prior to DXR. In general, subacute treatment provided greater efficiency in protecting against DXR-induced DNA damage in liver and kidney cells. These protective effects can be explained as the result of the phytochemicals present in açai pulp. These results will be applied to the developmental of food with functional characteristics, as well as to explore the characteristics of açai as a health promoter.

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Authors+Show Affiliations

Ribeiro JC
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Antunes LM
No affiliation info available
Aissa AF
No affiliation info available
Darin JD
No affiliation info available
De Rosso VV
No affiliation info available
Mercadante AZ
No affiliation info available
Bianchi Mde L
No affiliation info available

MeSH

AnimalsAnthocyaninsAntibiotics, AntineoplasticAntioxidantsArecaceaeBone MarrowComet AssayDNA DamageDose-Response Relationship, DrugDoxorubicinErythrocytesMaleMiceMicronucleus TestsPlant Extracts

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19892033

Citation

Ribeiro, Juliana Carvalho, et al. "Evaluation of the Genotoxic and Antigenotoxic Effects After Acute and Subacute Treatments With Açai Pulp (Euterpe Oleracea Mart.) On Mice Using the Erythrocytes Micronucleus Test and the Comet Assay." Mutation Research, vol. 695, no. 1-2, 2010, pp. 22-8.
Ribeiro JC, Antunes LM, Aissa AF, et al. Evaluation of the genotoxic and antigenotoxic effects after acute and subacute treatments with açai pulp (Euterpe oleracea Mart.) on mice using the erythrocytes micronucleus test and the comet assay. Mutat Res. 2010;695(1-2):22-8.
Ribeiro, J. C., Antunes, L. M., Aissa, A. F., Darin, J. D., De Rosso, V. V., Mercadante, A. Z., & Bianchi, M. d. e. . L. (2010). Evaluation of the genotoxic and antigenotoxic effects after acute and subacute treatments with açai pulp (Euterpe oleracea Mart.) on mice using the erythrocytes micronucleus test and the comet assay. Mutation Research, 695(1-2), 22-8. https://doi.org/10.1016/j.mrgentox.2009.10.009
Ribeiro JC, et al. Evaluation of the Genotoxic and Antigenotoxic Effects After Acute and Subacute Treatments With Açai Pulp (Euterpe Oleracea Mart.) On Mice Using the Erythrocytes Micronucleus Test and the Comet Assay. Mutat Res. 2010;695(1-2):22-8. PubMed PMID: 19892033.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the genotoxic and antigenotoxic effects after acute and subacute treatments with açai pulp (Euterpe oleracea Mart.) on mice using the erythrocytes micronucleus test and the comet assay. AU - Ribeiro,Juliana Carvalho, AU - Antunes,Lusânia Maria Greggi, AU - Aissa,Alexandre Ferro, AU - Darin,Joana D'arc Castania, AU - De Rosso,Veridiana Vera, AU - Mercadante,Adriana Zerlotti, AU - Bianchi,Maria de Lourdes Pires, Y1 - 2009/11/03/ PY - 2009/07/27/received PY - 2009/09/25/revised PY - 2009/10/20/accepted PY - 2009/11/7/entrez PY - 2009/11/7/pubmed PY - 2010/3/31/medline SP - 22 EP - 8 JF - Mutation research JO - Mutat Res VL - 695 IS - 1-2 N2 - Açai, the fruit of a palm native to the Amazonian basin, is widely distributed in northern South America, where it has considerable economic importance. Whereas individual polyphenolics compounds in açai have been extensively evaluated, studies of the intact fruit and its biological properties are lacking. Therefore, the present study was undertaken to investigate the in vivo genotoxicity of açai and its possible antigenotoxicity on doxorubicin (DXR)-induced DNA damage. The açai pulp doses selected were 3.33, 10.0 and 16.67g/kg b.w. administered by gavage alone or prior to DXR (16mg/kg b.w.) administered by intraperitoneal injection. Swiss albino mice were distributed in eight groups for acute treatment with açai pulp (24h) and eight groups for subacute treatment (daily for 14 consecutive days) before euthanasia. The negative control groups were treated in a similar way. The results of chemical analysis suggested the presence of carotenoids, anthocyanins, phenolic, and flavonoids in açai pulp. The endpoints analyzed were micronucleus induction in bone marrow and peripheral blood cells polychromatic erythrocytes, and DNA damage in peripheral blood, liver and kidney cells assessed using the alkaline (pH >13) comet assay. There were no statistically significant differences (p>0.05) between the negative control and the groups treated with the three doses of açai pulp alone in all endpoints analyzed, demonstrating the absence of genotoxic effects. The protective effects of açai pulp were observed in both acute and subacute treatments, when administered prior to DXR. In general, subacute treatment provided greater efficiency in protecting against DXR-induced DNA damage in liver and kidney cells. These protective effects can be explained as the result of the phytochemicals present in açai pulp. These results will be applied to the developmental of food with functional characteristics, as well as to explore the characteristics of açai as a health promoter. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/19892033/Evaluation_of_the_genotoxic_and_antigenotoxic_effects_after_acute_and_subacute_treatments_with_açai_pulp__Euterpe_oleracea_Mart___on_mice_using_the_erythrocytes_micronucleus_test_and_the_comet_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(09)00367-2 DB - PRIME DP - Unbound Medicine ER -