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[Leptospirosis screening: performance of the Serion Elisa Classic Leptospira IgM KIT].
Pathol Biol (Paris). 2010 Feb; 58(1):95-9.PB

Abstract

AIM OF THE STUDY

Evaluate the sensitivity and the specificity of anti-Leptospira IgM detection with the Serion Elisa classic kit in comparison to the Panbio Elisa kit.

METHODS

Twenty-three sera of patients whom Taqman probe real time PCR is positive and 19 sera positive by the microscopic agglutination test (MAT) are studied for sensitivity. Specificity is evaluated with 49 pairs of sera negative by MAT and with sera positive in IgM to EBV, to syphilis, to Borrelia and positive to influenza virus antibodies.

RESULTS

14/23 (61%) of the sera positive by PCR and 27/30 (90%) of the sera positive at significant level by MAT are positive by Serion IgM Elisa. Serogroups Icterohaemorrhagiae, Grippotyphosa, Australis, Tarassovi, Canicola, Sejroe, Patoc and Cynopteri are detected. 40/49 sera (82%) negative by MAT are negative by Serion IgM Elisa. Agreement with the Panbio kit is 61% (30/49). Results are discrepant in 19/49 cases corresponding to a positive result by Panbio IgM and to a negative result by Serion IgM. Serological cross-reactions are more frequent with IgM to syphilis and to Borrelia than with IgM to EBV and to influenza virus antibodies.

CONCLUSION

A similar sensitivity is observed between the two kits but specificity is higher with Serion. Positivity in IgM is 61% in cases of leptospirosis diagnosed by PCR.

Authors+Show Affiliations

Laboratoire Pasteur-Cerba, rue de l'Equerre, ZI des Béthunes, 95066 Cergy-Pontoise, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

fre

PubMed ID

19892494

Citation

Trombert-Paolantoni, S, et al. "[Leptospirosis Screening: Performance of the Serion Elisa Classic Leptospira IgM KIT]." Pathologie-biologie, vol. 58, no. 1, 2010, pp. 95-9.
Trombert-Paolantoni S, Thomas P, Hermet F, et al. [Leptospirosis screening: performance of the Serion Elisa Classic Leptospira IgM KIT]. Pathol Biol (Paris). 2010;58(1):95-9.
Trombert-Paolantoni, S., Thomas, P., Hermet, F., Clairet, V., Litou, N., & Maury, L. (2010). [Leptospirosis screening: performance of the Serion Elisa Classic Leptospira IgM KIT]. Pathologie-biologie, 58(1), 95-9. https://doi.org/10.1016/j.patbio.2009.06.008
Trombert-Paolantoni S, et al. [Leptospirosis Screening: Performance of the Serion Elisa Classic Leptospira IgM KIT]. Pathol Biol (Paris). 2010;58(1):95-9. PubMed PMID: 19892494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Leptospirosis screening: performance of the Serion Elisa Classic Leptospira IgM KIT]. AU - Trombert-Paolantoni,S, AU - Thomas,P, AU - Hermet,F, AU - Clairet,V, AU - Litou,N, AU - Maury,L, Y1 - 2009/11/04/ PY - 2009/05/28/received PY - 2009/06/26/accepted PY - 2009/11/7/entrez PY - 2009/11/7/pubmed PY - 2010/6/22/medline SP - 95 EP - 9 JF - Pathologie-biologie JO - Pathol Biol (Paris) VL - 58 IS - 1 N2 - AIM OF THE STUDY: Evaluate the sensitivity and the specificity of anti-Leptospira IgM detection with the Serion Elisa classic kit in comparison to the Panbio Elisa kit. METHODS: Twenty-three sera of patients whom Taqman probe real time PCR is positive and 19 sera positive by the microscopic agglutination test (MAT) are studied for sensitivity. Specificity is evaluated with 49 pairs of sera negative by MAT and with sera positive in IgM to EBV, to syphilis, to Borrelia and positive to influenza virus antibodies. RESULTS: 14/23 (61%) of the sera positive by PCR and 27/30 (90%) of the sera positive at significant level by MAT are positive by Serion IgM Elisa. Serogroups Icterohaemorrhagiae, Grippotyphosa, Australis, Tarassovi, Canicola, Sejroe, Patoc and Cynopteri are detected. 40/49 sera (82%) negative by MAT are negative by Serion IgM Elisa. Agreement with the Panbio kit is 61% (30/49). Results are discrepant in 19/49 cases corresponding to a positive result by Panbio IgM and to a negative result by Serion IgM. Serological cross-reactions are more frequent with IgM to syphilis and to Borrelia than with IgM to EBV and to influenza virus antibodies. CONCLUSION: A similar sensitivity is observed between the two kits but specificity is higher with Serion. Positivity in IgM is 61% in cases of leptospirosis diagnosed by PCR. SN - 1768-3114 UR - https://www.unboundmedicine.com/medline/citation/19892494/[Leptospirosis_screening:_performance_of_the_Serion_Elisa_Classic_Leptospira_IgM_KIT]_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0369-8114(09)00114-X DB - PRIME DP - Unbound Medicine ER -