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The neuroprotective effect of cannabidiol in an in vitro model of newborn hypoxic-ischemic brain damage in mice is mediated by CB(2) and adenosine receptors.
Neurobiol Dis 2010; 37(2):434-40ND

Abstract

To investigate the mechanisms involved in cannabidiol (CBD)-induced neuroprotection in hypoxic-ischemic (HI) immature brain, forebrain slices from newborn mice underwent oxygen and glucose deprivation in the presence of vehicle, or CBD alone or with selective antagonists of cannabinoid CB(1) and CB(2), and adenosine A(1) and A(2) receptors. CBD reduced acute (LDH efflux to the incubation medium) and apoptotic (caspase-9 concentration in tissue) HI brain damage by reducing glutamate and IL-6 concentration, and TNFalpha, COX-2, and iNOS expression. CBD effects were reversed by the CB(2) antagonist AM630 and by the A(2A) antagonist SCH58261. The A(1A) antagonist DPCPX only counteracted the CBD reduction of glutamate release, while the CB(1) antagonist SR141716 did not modify any effect of CBD. In conclusion, CBD induces robust neuroprotection in immature brain, by acting on some of the major mechanisms underlying HI cell death; these effects are mediated by CB(2) and adenosine, mainly A(2A), receptors.

Authors+Show Affiliations

Laboratorio de Apoyo a la Investigación, Hospital Universitario Fundación Alcorcón, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19900555

Citation

Castillo, A, et al. "The Neuroprotective Effect of Cannabidiol in an in Vitro Model of Newborn Hypoxic-ischemic Brain Damage in Mice Is Mediated By CB(2) and Adenosine Receptors." Neurobiology of Disease, vol. 37, no. 2, 2010, pp. 434-40.
Castillo A, Tolón MR, Fernández-Ruiz J, et al. The neuroprotective effect of cannabidiol in an in vitro model of newborn hypoxic-ischemic brain damage in mice is mediated by CB(2) and adenosine receptors. Neurobiol Dis. 2010;37(2):434-40.
Castillo, A., Tolón, M. R., Fernández-Ruiz, J., Romero, J., & Martinez-Orgado, J. (2010). The neuroprotective effect of cannabidiol in an in vitro model of newborn hypoxic-ischemic brain damage in mice is mediated by CB(2) and adenosine receptors. Neurobiology of Disease, 37(2), pp. 434-40. doi:10.1016/j.nbd.2009.10.023.
Castillo A, et al. The Neuroprotective Effect of Cannabidiol in an in Vitro Model of Newborn Hypoxic-ischemic Brain Damage in Mice Is Mediated By CB(2) and Adenosine Receptors. Neurobiol Dis. 2010;37(2):434-40. PubMed PMID: 19900555.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The neuroprotective effect of cannabidiol in an in vitro model of newborn hypoxic-ischemic brain damage in mice is mediated by CB(2) and adenosine receptors. AU - Castillo,A, AU - Tolón,M R, AU - Fernández-Ruiz,J, AU - Romero,J, AU - Martinez-Orgado,J, Y1 - 2009/11/06/ PY - 2009/08/03/received PY - 2009/10/22/revised PY - 2009/10/26/accepted PY - 2009/11/11/entrez PY - 2009/11/11/pubmed PY - 2010/6/16/medline SP - 434 EP - 40 JF - Neurobiology of disease JO - Neurobiol. Dis. VL - 37 IS - 2 N2 - To investigate the mechanisms involved in cannabidiol (CBD)-induced neuroprotection in hypoxic-ischemic (HI) immature brain, forebrain slices from newborn mice underwent oxygen and glucose deprivation in the presence of vehicle, or CBD alone or with selective antagonists of cannabinoid CB(1) and CB(2), and adenosine A(1) and A(2) receptors. CBD reduced acute (LDH efflux to the incubation medium) and apoptotic (caspase-9 concentration in tissue) HI brain damage by reducing glutamate and IL-6 concentration, and TNFalpha, COX-2, and iNOS expression. CBD effects were reversed by the CB(2) antagonist AM630 and by the A(2A) antagonist SCH58261. The A(1A) antagonist DPCPX only counteracted the CBD reduction of glutamate release, while the CB(1) antagonist SR141716 did not modify any effect of CBD. In conclusion, CBD induces robust neuroprotection in immature brain, by acting on some of the major mechanisms underlying HI cell death; these effects are mediated by CB(2) and adenosine, mainly A(2A), receptors. SN - 1095-953X UR - https://www.unboundmedicine.com/medline/citation/19900555/abstract/The_neuroprotective_effect_of_cannabidiol_in_an_in_vitro_model_of_newborn_hypoxic_ischemic_brain_damage_in_mice_is_mediated_by_CB_2__and_adenosine_receptors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-9961(09)00309-X DB - PRIME DP - Unbound Medicine ER -