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Plant isoprenoid biosynthesis via the MEP pathway: in vivo IPP/DMAPP ratio produced by (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase in tobacco BY-2 cell cultures.
FEBS Lett. 2010 Jan 04; 584(1):129-34.FL

Abstract

Feeding tobacco BY-2 cells with [2-(13)C,4-(2)H]deoxyxylulose revealed from the (13)C labeling that the plastid isoprenoids, synthesized via the MEP pathway, are essentially derived from the labeled precursor. The ca. 15% (2)H retention observed in all isoprene units corresponds to the isopentenyl diphosphate (IPP)/dimethylallyl diphosphate (DMAPP) ratio (85:15) directly produced by the hydroxymethylbutenyl diphosphate reductase, the last enzyme of the MEP pathway. (2)H retention characterizes the isoprene units derived from the DMAPP branch, whereas (2)H loss represents the signature of the IPP branch. Taking into account the enantioselectivity of the reactions catalyzed by the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, the IPP isomerase and the trans-prenyl transferase, a single biogenetic scheme allows to interpret all labeling patterns observed in bacteria or plants upon incubation with (2)H labeled deoxyxylulose.

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Authors+Show Affiliations

Tritsch D
Université de Strasbourg/CNRS, Institut de Chimie UMR 7177, Strasbourg, France.
Hemmerlin A
No affiliation info available
Bach TJ
No affiliation info available
Rohmer M
No affiliation info available

MeSH

Cell Culture TechniquesCells, CulturedErythritolHemiterpenesOrganophosphorus CompoundsPlastidsSugar PhosphatesTerpenesTobaccoXylulose

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19903472

Citation

Tritsch, Denis, et al. "Plant Isoprenoid Biosynthesis Via the MEP Pathway: in Vivo IPP/DMAPP Ratio Produced By (E)-4-hydroxy-3-methylbut-2-enyl Diphosphate Reductase in Tobacco BY-2 Cell Cultures." FEBS Letters, vol. 584, no. 1, 2010, pp. 129-34.
Tritsch D, Hemmerlin A, Bach TJ, et al. Plant isoprenoid biosynthesis via the MEP pathway: in vivo IPP/DMAPP ratio produced by (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase in tobacco BY-2 cell cultures. FEBS Lett. 2010;584(1):129-34.
Tritsch, D., Hemmerlin, A., Bach, T. J., & Rohmer, M. (2010). Plant isoprenoid biosynthesis via the MEP pathway: in vivo IPP/DMAPP ratio produced by (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase in tobacco BY-2 cell cultures. FEBS Letters, 584(1), 129-34. https://doi.org/10.1016/j.febslet.2009.11.010
Tritsch D, et al. Plant Isoprenoid Biosynthesis Via the MEP Pathway: in Vivo IPP/DMAPP Ratio Produced By (E)-4-hydroxy-3-methylbut-2-enyl Diphosphate Reductase in Tobacco BY-2 Cell Cultures. FEBS Lett. 2010 Jan 4;584(1):129-34. PubMed PMID: 19903472.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plant isoprenoid biosynthesis via the MEP pathway: in vivo IPP/DMAPP ratio produced by (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase in tobacco BY-2 cell cultures. AU - Tritsch,Denis, AU - Hemmerlin,Andréa, AU - Bach,Thomas J, AU - Rohmer,Michel, PY - 2009/09/04/received PY - 2009/11/04/accepted PY - 2009/11/12/entrez PY - 2009/11/12/pubmed PY - 2010/1/22/medline SP - 129 EP - 34 JF - FEBS letters JO - FEBS Lett VL - 584 IS - 1 N2 - Feeding tobacco BY-2 cells with [2-(13)C,4-(2)H]deoxyxylulose revealed from the (13)C labeling that the plastid isoprenoids, synthesized via the MEP pathway, are essentially derived from the labeled precursor. The ca. 15% (2)H retention observed in all isoprene units corresponds to the isopentenyl diphosphate (IPP)/dimethylallyl diphosphate (DMAPP) ratio (85:15) directly produced by the hydroxymethylbutenyl diphosphate reductase, the last enzyme of the MEP pathway. (2)H retention characterizes the isoprene units derived from the DMAPP branch, whereas (2)H loss represents the signature of the IPP branch. Taking into account the enantioselectivity of the reactions catalyzed by the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, the IPP isomerase and the trans-prenyl transferase, a single biogenetic scheme allows to interpret all labeling patterns observed in bacteria or plants upon incubation with (2)H labeled deoxyxylulose. SN - 1873-3468 UR - https://www.unboundmedicine.com/medline/citation/19903472/Plant_isoprenoid_biosynthesis_via_the_MEP_pathway:_in_vivo_IPP/DMAPP_ratio_produced_by__E__4_hydroxy_3_methylbut_2_enyl_diphosphate_reductase_in_tobacco_BY_2_cell_cultures_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(09)00903-X DB - PRIME DP - Unbound Medicine ER -