Tags

Type your tag names separated by a space and hit enter

Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher.
Appl Opt 2009; 48(32):6344-54AO

Abstract

The optical stretcher is a dual-beam trap capable of stretching individual cells. Previous studies have used either ray- or wave-optical models to compute the optical pressure on the surface of a spherical cell. We have extended the ray-optics model to account for focusing by the spherical interface and the effects of multiple internal reflections. Simulation results for red-blood cells (RBCs) show that internal reflections can lead to significant perturbation of the deformation, leading to a systematic error in the determination of cellular elasticity. Calibration studies show excellent agreement between the predicted and measured escape force, and RBC stiffness measurements are consistent with literature values. Measurements of the elasticity of murine osteogenic cells reveal that these cells are approximately 5.4 times stiffer than RBCs.

Authors+Show Affiliations

Department of Physics, Creighton University, 2500 California Plaza, Omaha, Nebraska 68178, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19904335

Citation

Ekpenyong, Andrew E., et al. "Determination of Cell Elasticity Through Hybrid Ray Optics and Continuum Mechanics Modeling of Cell Deformation in the Optical Stretcher." Applied Optics, vol. 48, no. 32, 2009, pp. 6344-54.
Ekpenyong AE, Posey CL, Chaput JL, et al. Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher. Appl Opt. 2009;48(32):6344-54.
Ekpenyong, A. E., Posey, C. L., Chaput, J. L., Burkart, A. K., Marquardt, M. M., Smith, T. J., & Nichols, M. G. (2009). Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher. Applied Optics, 48(32), pp. 6344-54. doi:10.1364/AO.48.006344.
Ekpenyong AE, et al. Determination of Cell Elasticity Through Hybrid Ray Optics and Continuum Mechanics Modeling of Cell Deformation in the Optical Stretcher. Appl Opt. 2009 Nov 10;48(32):6344-54. PubMed PMID: 19904335.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher. AU - Ekpenyong,Andrew E, AU - Posey,Carolyn L, AU - Chaput,Joy L, AU - Burkart,Anya K, AU - Marquardt,Meg M, AU - Smith,Timothy J, AU - Nichols,Michael G, PY - 2009/11/12/entrez PY - 2009/11/12/pubmed PY - 2010/1/21/medline SP - 6344 EP - 54 JF - Applied optics JO - Appl Opt VL - 48 IS - 32 N2 - The optical stretcher is a dual-beam trap capable of stretching individual cells. Previous studies have used either ray- or wave-optical models to compute the optical pressure on the surface of a spherical cell. We have extended the ray-optics model to account for focusing by the spherical interface and the effects of multiple internal reflections. Simulation results for red-blood cells (RBCs) show that internal reflections can lead to significant perturbation of the deformation, leading to a systematic error in the determination of cellular elasticity. Calibration studies show excellent agreement between the predicted and measured escape force, and RBC stiffness measurements are consistent with literature values. Measurements of the elasticity of murine osteogenic cells reveal that these cells are approximately 5.4 times stiffer than RBCs. SN - 1539-4522 UR - https://www.unboundmedicine.com/medline/citation/19904335/Determination_of_cell_elasticity_through_hybrid_ray_optics_and_continuum_mechanics_modeling_of_cell_deformation_in_the_optical_stretcher_ L2 - http://www.opticsinfobase.org/abstract.cfm?URI=ao-48-32-6344 DB - PRIME DP - Unbound Medicine ER -