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The role of two putative nitroreductases, Frm2p and Hbn1p, in the oxidative stress response in Saccharomyces cerevisiae.
Yeast. 2010 Feb; 27(2):89-102.Y

Abstract

The nitroreductase family is comprised of a group of FMN- or FAD-dependent enzymes that are able to metabolize nitrosubstituted compounds using the reducing power of NAD(P)H. These nitroreductases can be found in bacterial species and, to a lesser extent, in eukaryotes. There is little information on the biochemical functions of nitroreductases. Some studies suggest their possible involvement in the oxidative stress response. In the yeast Saccharomyces cerevisiae, two nitroreductase proteins, Frm2p and Hbn1p, have been described. While Frm2p appears to act in the lipid signalling pathway, the function of Hbn1p is completely unknown. In order to elucidate the functions of Frm2p and Hbn1p, we evaluated the sensitivity of yeast strains, proficient and deficient in both oxidative stress proteins, for respiratory competence, antioxidant-enzyme activities, intracellular reactive oxygen species (ROS) production and lipid peroxidation. We found reduced basal activity of superoxide dismutase (SOD), ROS production, lipid peroxidation and petite induction and higher sensitivity to 4-nitroquinoline-oxide (4-NQO) and N-nitrosodiethylamine (NDEA), as well as higher basal activity of catalase (CAT) and glutathione peroxidase (GPx) and reduced glutathione (GSH) content in the single and double mutant strains frm2Delta and frm2Delta hbn1Delta. These strains exhibited less ROS accumulation and lipid peroxidation when exposed to peroxides, H(2)O(2) and t-BOOH. In summary, the Frm1p and Hbn1p nitroreductases influence the response to oxidative stress in S. cerevisae yeast by modulating the GSH contents and antioxidant enzymatic activities, such as SOD, CAT and GPx.

Authors+Show Affiliations

Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av Bento Gonçalves 9500, 91507-970 Porto Alegre, RS, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19904831

Citation

de Oliveira, Iuri Marques, et al. "The Role of Two Putative Nitroreductases, Frm2p and Hbn1p, in the Oxidative Stress Response in Saccharomyces Cerevisiae." Yeast (Chichester, England), vol. 27, no. 2, 2010, pp. 89-102.
de Oliveira IM, Zanotto-Filho A, Moreira JC, et al. The role of two putative nitroreductases, Frm2p and Hbn1p, in the oxidative stress response in Saccharomyces cerevisiae. Yeast. 2010;27(2):89-102.
de Oliveira, I. M., Zanotto-Filho, A., Moreira, J. C., Bonatto, D., & Henriques, J. A. (2010). The role of two putative nitroreductases, Frm2p and Hbn1p, in the oxidative stress response in Saccharomyces cerevisiae. Yeast (Chichester, England), 27(2), 89-102. https://doi.org/10.1002/yea.1734
de Oliveira IM, et al. The Role of Two Putative Nitroreductases, Frm2p and Hbn1p, in the Oxidative Stress Response in Saccharomyces Cerevisiae. Yeast. 2010;27(2):89-102. PubMed PMID: 19904831.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of two putative nitroreductases, Frm2p and Hbn1p, in the oxidative stress response in Saccharomyces cerevisiae. AU - de Oliveira,Iuri Marques, AU - Zanotto-Filho,Alfeu, AU - Moreira,José Cláudio Fonseca, AU - Bonatto,Diego, AU - Henriques,João Antonio Pêgas, PY - 2009/11/12/entrez PY - 2009/11/12/pubmed PY - 2010/3/27/medline SP - 89 EP - 102 JF - Yeast (Chichester, England) JO - Yeast VL - 27 IS - 2 N2 - The nitroreductase family is comprised of a group of FMN- or FAD-dependent enzymes that are able to metabolize nitrosubstituted compounds using the reducing power of NAD(P)H. These nitroreductases can be found in bacterial species and, to a lesser extent, in eukaryotes. There is little information on the biochemical functions of nitroreductases. Some studies suggest their possible involvement in the oxidative stress response. In the yeast Saccharomyces cerevisiae, two nitroreductase proteins, Frm2p and Hbn1p, have been described. While Frm2p appears to act in the lipid signalling pathway, the function of Hbn1p is completely unknown. In order to elucidate the functions of Frm2p and Hbn1p, we evaluated the sensitivity of yeast strains, proficient and deficient in both oxidative stress proteins, for respiratory competence, antioxidant-enzyme activities, intracellular reactive oxygen species (ROS) production and lipid peroxidation. We found reduced basal activity of superoxide dismutase (SOD), ROS production, lipid peroxidation and petite induction and higher sensitivity to 4-nitroquinoline-oxide (4-NQO) and N-nitrosodiethylamine (NDEA), as well as higher basal activity of catalase (CAT) and glutathione peroxidase (GPx) and reduced glutathione (GSH) content in the single and double mutant strains frm2Delta and frm2Delta hbn1Delta. These strains exhibited less ROS accumulation and lipid peroxidation when exposed to peroxides, H(2)O(2) and t-BOOH. In summary, the Frm1p and Hbn1p nitroreductases influence the response to oxidative stress in S. cerevisae yeast by modulating the GSH contents and antioxidant enzymatic activities, such as SOD, CAT and GPx. SN - 1097-0061 UR - https://www.unboundmedicine.com/medline/citation/19904831/The_role_of_two_putative_nitroreductases_Frm2p_and_Hbn1p_in_the_oxidative_stress_response_in_Saccharomyces_cerevisiae_ L2 - https://doi.org/10.1002/yea.1734 DB - PRIME DP - Unbound Medicine ER -