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Studies on the antioxidant activity and phenolic compounds of enzyme-assisted water extracts from Du-zhong (Eucommia ulmoides Oliv.) leaves.
J Enzyme Inhib Med Chem. 2009 Dec; 24(6):1280-7.JE

Abstract

Enzyme-assisted water extracts (EWEDL) and ethanol extracts of Du-zhong leaves (EEDL) were evaluated for their antioxidant activities using the DPPH radical-scavenging assay, Fe(2+)-chelating assay, and inhibition ability of the linoleic acid peroxidation assay. In general, the antioxidant activity of Du-zhong leaf extracts increased with increasing concentration. Based on the two extracting methods with different antioxidative reactions, it was shown that the enzyme-assisted water extracting method was more effective for antioxidant extraction from Du-zhong leaves. By HPLC-MS analysis, the main phenolic compounds (geniposidic acid, epicatechin, and chlorogenic acid) identified in EWEDL and EEDL were similar. EWEDL and EEDL had total phenolic contents of 13.84 +/- 0.11 and 14.72 +/- 0.14 mg chlorogenic acid equivalents (CAE) in each gram of extract, respectively. However, there was no positive correlation between total phenolic content and antioxidant activities of EWEDL and EEDL measured by the three different assays.

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Authors+Show Affiliations

Luo J
Department of Food Engineering, Shaanxi Normal University, Xi'an, People's Republic of China. luojy@snnu.edu.cn
Tian C
No affiliation info available
Xu J
No affiliation info available
Sun Y
No affiliation info available

MeSH

AntioxidantsCellulaseEucommiaceaeFree Radical ScavengersIron Chelating AgentsLinoleic AcidLipid PeroxidationPhenolsPlant ExtractsPlant LeavesWater

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19912062

Citation

Luo, Jiyang, et al. "Studies On the Antioxidant Activity and Phenolic Compounds of Enzyme-assisted Water Extracts From Du-zhong (Eucommia Ulmoides Oliv.) Leaves." Journal of Enzyme Inhibition and Medicinal Chemistry, vol. 24, no. 6, 2009, pp. 1280-7.
Luo J, Tian C, Xu J, et al. Studies on the antioxidant activity and phenolic compounds of enzyme-assisted water extracts from Du-zhong (Eucommia ulmoides Oliv.) leaves. J Enzyme Inhib Med Chem. 2009;24(6):1280-7.
Luo, J., Tian, C., Xu, J., & Sun, Y. (2009). Studies on the antioxidant activity and phenolic compounds of enzyme-assisted water extracts from Du-zhong (Eucommia ulmoides Oliv.) leaves. Journal of Enzyme Inhibition and Medicinal Chemistry, 24(6), 1280-7. https://doi.org/10.3109/14756360902829741
Luo J, et al. Studies On the Antioxidant Activity and Phenolic Compounds of Enzyme-assisted Water Extracts From Du-zhong (Eucommia Ulmoides Oliv.) Leaves. J Enzyme Inhib Med Chem. 2009;24(6):1280-7. PubMed PMID: 19912062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on the antioxidant activity and phenolic compounds of enzyme-assisted water extracts from Du-zhong (Eucommia ulmoides Oliv.) leaves. AU - Luo,Jiyang, AU - Tian,Chengrui, AU - Xu,Jianguo, AU - Sun,Ye, PY - 2009/11/17/entrez PY - 2009/11/17/pubmed PY - 2010/4/7/medline SP - 1280 EP - 7 JF - Journal of enzyme inhibition and medicinal chemistry JO - J Enzyme Inhib Med Chem VL - 24 IS - 6 N2 - Enzyme-assisted water extracts (EWEDL) and ethanol extracts of Du-zhong leaves (EEDL) were evaluated for their antioxidant activities using the DPPH radical-scavenging assay, Fe(2+)-chelating assay, and inhibition ability of the linoleic acid peroxidation assay. In general, the antioxidant activity of Du-zhong leaf extracts increased with increasing concentration. Based on the two extracting methods with different antioxidative reactions, it was shown that the enzyme-assisted water extracting method was more effective for antioxidant extraction from Du-zhong leaves. By HPLC-MS analysis, the main phenolic compounds (geniposidic acid, epicatechin, and chlorogenic acid) identified in EWEDL and EEDL were similar. EWEDL and EEDL had total phenolic contents of 13.84 +/- 0.11 and 14.72 +/- 0.14 mg chlorogenic acid equivalents (CAE) in each gram of extract, respectively. However, there was no positive correlation between total phenolic content and antioxidant activities of EWEDL and EEDL measured by the three different assays. SN - 1475-6374 UR - https://www.unboundmedicine.com/medline/citation/19912062/Studies_on_the_antioxidant_activity_and_phenolic_compounds_of_enzyme_assisted_water_extracts_from_Du_zhong__Eucommia_ulmoides_Oliv___leaves_ L2 - https://www.tandfonline.com/doi/full/10.3109/14756360902829741 DB - PRIME DP - Unbound Medicine ER -