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Curcumin pretreatment protects against acute acrylonitrile-induced oxidative damage in rats.
Toxicology. 2010 Jan 12; 267(1-3):140-6.T

Abstract

Acrylonitrile (AN) is widely used in the manufacturing of fibers, plastics and pharmaceuticals. Free radical-mediated lipid peroxidation is implicated in the toxicity of AN. The present study was designed to examine the ability of curcumin, a natural polyphenolic compound, to attenuate acute AN-induced lipid peroxidation in the brain and liver of rats. Male Sprague-Dawley rats were orally administered curcumin at doses of 0 (olive oil control), 50 or 100 mg/kg bodyweight daily for 7 consecutive days. Two hours after the last dose of curcumin, rats received an intraperitoneal injection of 50 mg AN/kg bodyweight. Acute exposure to AN significantly increased the generation of lipid peroxidation products, reflected by high levels of malondialdehyde (MDA) both in the brain and liver. These increases were accompanied by a significant decrease in reduced glutathione (GSH) content and a significant reduction in catalase (CAT) activity in the same tissues. No consistent changes in superoxide dismutase (SOD) activity were observed between the control and AN-treatment groups in both tissues. Pretreatment with curcumin reversed the AN-induced effects, reducing the levels of MDA and enhancing CAT activity and increasing reduced GSH content both in the brain and liver. Furthermore, curcumin effectively prevented AN-induced decrease in cytochrome c oxidase activity in both liver and brain. These results establish that curcumin pretreatment has a beneficial role in mitigating AN-induced oxidative stress both in the brains and livers of exposed rats and these effects are mediated independently of cytochrome P450 2E1 inhibition. Accordingly, curcumin should be considered as a potential safe and effective approach in attenuating the adverse effects produced by AN-related toxicants.

Authors+Show Affiliations

Department of Preventive Medicine, School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19913070

Citation

Guangwei, Xing, et al. "Curcumin Pretreatment Protects Against Acute Acrylonitrile-induced Oxidative Damage in Rats." Toxicology, vol. 267, no. 1-3, 2010, pp. 140-6.
Guangwei X, Rongzhu L, Wenrong X, et al. Curcumin pretreatment protects against acute acrylonitrile-induced oxidative damage in rats. Toxicology. 2010;267(1-3):140-6.
Guangwei, X., Rongzhu, L., Wenrong, X., Suhua, W., Xiaowu, Z., Shizhong, W., Ye, Z., Aschner, M., Kulkarni, S. K., & Bishnoi, M. (2010). Curcumin pretreatment protects against acute acrylonitrile-induced oxidative damage in rats. Toxicology, 267(1-3), 140-6. https://doi.org/10.1016/j.tox.2009.11.001
Guangwei X, et al. Curcumin Pretreatment Protects Against Acute Acrylonitrile-induced Oxidative Damage in Rats. Toxicology. 2010 Jan 12;267(1-3):140-6. PubMed PMID: 19913070.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Curcumin pretreatment protects against acute acrylonitrile-induced oxidative damage in rats. AU - Guangwei,Xing, AU - Rongzhu,Lu, AU - Wenrong,Xu, AU - Suhua,Wang, AU - Xiaowu,Zhao, AU - Shizhong,Wang, AU - Ye,Zhang, AU - Aschner,Michael, AU - Kulkarni,Shrinivas K, AU - Bishnoi,Mahendra, Y1 - 2009/11/11/ PY - 2009/08/16/received PY - 2009/10/09/revised PY - 2009/11/02/accepted PY - 2009/11/17/entrez PY - 2009/11/17/pubmed PY - 2010/2/27/medline SP - 140 EP - 6 JF - Toxicology JO - Toxicology VL - 267 IS - 1-3 N2 - Acrylonitrile (AN) is widely used in the manufacturing of fibers, plastics and pharmaceuticals. Free radical-mediated lipid peroxidation is implicated in the toxicity of AN. The present study was designed to examine the ability of curcumin, a natural polyphenolic compound, to attenuate acute AN-induced lipid peroxidation in the brain and liver of rats. Male Sprague-Dawley rats were orally administered curcumin at doses of 0 (olive oil control), 50 or 100 mg/kg bodyweight daily for 7 consecutive days. Two hours after the last dose of curcumin, rats received an intraperitoneal injection of 50 mg AN/kg bodyweight. Acute exposure to AN significantly increased the generation of lipid peroxidation products, reflected by high levels of malondialdehyde (MDA) both in the brain and liver. These increases were accompanied by a significant decrease in reduced glutathione (GSH) content and a significant reduction in catalase (CAT) activity in the same tissues. No consistent changes in superoxide dismutase (SOD) activity were observed between the control and AN-treatment groups in both tissues. Pretreatment with curcumin reversed the AN-induced effects, reducing the levels of MDA and enhancing CAT activity and increasing reduced GSH content both in the brain and liver. Furthermore, curcumin effectively prevented AN-induced decrease in cytochrome c oxidase activity in both liver and brain. These results establish that curcumin pretreatment has a beneficial role in mitigating AN-induced oxidative stress both in the brains and livers of exposed rats and these effects are mediated independently of cytochrome P450 2E1 inhibition. Accordingly, curcumin should be considered as a potential safe and effective approach in attenuating the adverse effects produced by AN-related toxicants. SN - 1879-3185 UR - https://www.unboundmedicine.com/medline/citation/19913070/Curcumin_pretreatment_protects_against_acute_acrylonitrile_induced_oxidative_damage_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-483X(09)00571-X DB - PRIME DP - Unbound Medicine ER -