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Improved method for identifying and quantifying olive oil phenolic compounds and their metabolites in human plasma by microelution solid-phase extraction plate and liquid chromatography-tandem mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Dec 15; 877(32):4097-106.JC

Abstract

Two methods based on solid-phase extraction (SPE) using traditional cartridges and microelution SPE plates (muSPE) as the sample pre-treatment, and an improved liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) were developed and compared to determine the phenolic compounds in virgin oil olive from plasma samples. The phenolic compounds studied were hydroxytyrosol, tyrosol, homovanillic acid, p-coumaric acid, 3,4-DHPEA-EDA, p-HPEA-EDA, luteolin, apigenin, pinoresinol and acetoxypinoresinol. Good recoveries were obtained in both methods, and the LOQs and LODs were similar, in the range of low muM. The advantage of muSPE, in comparison with SPE cartridges, was the lack of the evaporation step to pre-concentrate the analytes. The muSPE-UPLC-ESI-MS/MS method developed was then applied to determine the phenolic compounds and their metabolites, in glucuronide, sulphate and methylated forms, in human plasma after the ingestion of virgin olive oil.

Authors+Show Affiliations

Food Technology Department, XaRTA-TPV, Escuela Técnica Superior de Ingeniería Agraria, University of Lleida, Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

19913464

Citation

Suárez, Manuel, et al. "Improved Method for Identifying and Quantifying Olive Oil Phenolic Compounds and Their Metabolites in Human Plasma By Microelution Solid-phase Extraction Plate and Liquid Chromatography-tandem Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 32, 2009, pp. 4097-106.
Suárez M, Romero MP, Macià A, et al. Improved method for identifying and quantifying olive oil phenolic compounds and their metabolites in human plasma by microelution solid-phase extraction plate and liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877(32):4097-106.
Suárez, M., Romero, M. P., Macià, A., Valls, R. M., Fernández, S., Solà, R., & Motilva, M. J. (2009). Improved method for identifying and quantifying olive oil phenolic compounds and their metabolites in human plasma by microelution solid-phase extraction plate and liquid chromatography-tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877(32), 4097-106. https://doi.org/10.1016/j.jchromb.2009.10.025
Suárez M, et al. Improved Method for Identifying and Quantifying Olive Oil Phenolic Compounds and Their Metabolites in Human Plasma By Microelution Solid-phase Extraction Plate and Liquid Chromatography-tandem Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Dec 15;877(32):4097-106. PubMed PMID: 19913464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved method for identifying and quantifying olive oil phenolic compounds and their metabolites in human plasma by microelution solid-phase extraction plate and liquid chromatography-tandem mass spectrometry. AU - Suárez,Manuel, AU - Romero,Maria-Paz, AU - Macià,Alba, AU - Valls,Rosa M, AU - Fernández,Sara, AU - Solà,Rosa, AU - Motilva,Maria-José, Y1 - 2009/10/30/ PY - 2009/07/27/received PY - 2009/10/01/revised PY - 2009/10/24/accepted PY - 2009/11/17/entrez PY - 2009/11/17/pubmed PY - 2010/2/6/medline SP - 4097 EP - 106 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 877 IS - 32 N2 - Two methods based on solid-phase extraction (SPE) using traditional cartridges and microelution SPE plates (muSPE) as the sample pre-treatment, and an improved liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) were developed and compared to determine the phenolic compounds in virgin oil olive from plasma samples. The phenolic compounds studied were hydroxytyrosol, tyrosol, homovanillic acid, p-coumaric acid, 3,4-DHPEA-EDA, p-HPEA-EDA, luteolin, apigenin, pinoresinol and acetoxypinoresinol. Good recoveries were obtained in both methods, and the LOQs and LODs were similar, in the range of low muM. The advantage of muSPE, in comparison with SPE cartridges, was the lack of the evaporation step to pre-concentrate the analytes. The muSPE-UPLC-ESI-MS/MS method developed was then applied to determine the phenolic compounds and their metabolites, in glucuronide, sulphate and methylated forms, in human plasma after the ingestion of virgin olive oil. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/19913464/Improved_method_for_identifying_and_quantifying_olive_oil_phenolic_compounds_and_their_metabolites_in_human_plasma_by_microelution_solid_phase_extraction_plate_and_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(09)00740-5 DB - PRIME DP - Unbound Medicine ER -