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The transcription activation domain of Rpn4 is separate from its degrons.
Int J Biochem Cell Biol. 2010 Feb; 42(2):282-6.IJ

Abstract

The proteasome homeostasis in Saccharomyces cerevisiae is regulated by a negative feedback circuit in which the transcription activator Rpn4 upregulates the proteasome genes and is rapidly degraded by the assembled proteasome. In addition to the proteasome genes, Rpn4 regulates numerous other genes involved in a wide variety of cellular processes. However, the transcription activation domain of Rpn4 remains largely unclear. Here we locate a major transactivation domain of Rpn4 in the N-terminal region between residues 118 and 210. Interestingly, this domain is separate from its degradation signals (degrons), suggesting that functional overlap of sequences that activate transcription and signal degradation may not be as common as previously thought. We further demonstrate that the intracellular proteasome activity is correlated with the transactivation potency of Rpn4. This study provides important information for further understanding the biological functions of Rpn4 and the proteasome system.

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Authors+Show Affiliations

Ju D
Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E. Warren Avenue, Detroit, MI 48201, USA.
Xu H
No affiliation info available
Wang X
No affiliation info available
Xie Y
No affiliation info available

MeSH

Active Transport, Cell NucleusDNA-Binding ProteinsIntracellular SpaceProteasome Endopeptidase ComplexProtein Structure, TertiarySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsTranscriptional Activation

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19914394

Citation

Ju, Donghong, et al. "The Transcription Activation Domain of Rpn4 Is Separate From Its Degrons." The International Journal of Biochemistry & Cell Biology, vol. 42, no. 2, 2010, pp. 282-6.
Ju D, Xu H, Wang X, et al. The transcription activation domain of Rpn4 is separate from its degrons. Int J Biochem Cell Biol. 2010;42(2):282-6.
Ju, D., Xu, H., Wang, X., & Xie, Y. (2010). The transcription activation domain of Rpn4 is separate from its degrons. The International Journal of Biochemistry & Cell Biology, 42(2), 282-6. https://doi.org/10.1016/j.biocel.2009.11.003
Ju D, et al. The Transcription Activation Domain of Rpn4 Is Separate From Its Degrons. Int J Biochem Cell Biol. 2010;42(2):282-6. PubMed PMID: 19914394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The transcription activation domain of Rpn4 is separate from its degrons. AU - Ju,Donghong, AU - Xu,Haiming, AU - Wang,Xiaogang, AU - Xie,Youming, Y1 - 2009/11/13/ PY - 2009/08/25/received PY - 2009/10/17/revised PY - 2009/11/05/accepted PY - 2009/11/17/entrez PY - 2009/11/17/pubmed PY - 2010/4/30/medline SP - 282 EP - 6 JF - The international journal of biochemistry & cell biology JO - Int J Biochem Cell Biol VL - 42 IS - 2 N2 - The proteasome homeostasis in Saccharomyces cerevisiae is regulated by a negative feedback circuit in which the transcription activator Rpn4 upregulates the proteasome genes and is rapidly degraded by the assembled proteasome. In addition to the proteasome genes, Rpn4 regulates numerous other genes involved in a wide variety of cellular processes. However, the transcription activation domain of Rpn4 remains largely unclear. Here we locate a major transactivation domain of Rpn4 in the N-terminal region between residues 118 and 210. Interestingly, this domain is separate from its degradation signals (degrons), suggesting that functional overlap of sequences that activate transcription and signal degradation may not be as common as previously thought. We further demonstrate that the intracellular proteasome activity is correlated with the transactivation potency of Rpn4. This study provides important information for further understanding the biological functions of Rpn4 and the proteasome system. SN - 1878-5875 UR - https://www.unboundmedicine.com/medline/citation/19914394/The_transcription_activation_domain_of_Rpn4_is_separate_from_its_degrons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1357-2725(09)00318-5 DB - PRIME DP - Unbound Medicine ER -