Tags

Type your tag names separated by a space and hit enter

Aptamer based electrochemical assay for the determination of thrombin by using the amplification of the nanoparticles.
Biosens Bioelectron. 2010 Feb 15; 25(6):1290-4.BB

Abstract

A novel electrochemical assay based on the aptamer and the signal of amplification of nanoparticles (NPs) was constructed for the determination of thrombin. Aptamers immobilized on the electrode and Au NPs could be assembled with the target protein to form a sandwich structure in the presence of the latter. Differential pulse voltammetry (DPV) was employed to detect the CdS NPs loaded on the surface of the Au NPs through the linker DNA, which was related to the concentration of the target protein. The assay took advantage of the amplification ability of Au nanoparticles carrying multiplex CdS NPs and the specific affinity of aptamers. Thrombin was detected in this assay in the linear range of 1.0x10(-15) to 1.0x10(-11) M with the detection limit of 5.5x10(-16) M of target protein. In addition, the assay could be used to detection thrombin in real samples with high sensitivity and good selectivity.

Links

Publisher Full Text

Authors+Show Affiliations

Ding C
The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, PR China.
Ge Y
No affiliation info available
Lin JM
No affiliation info available

MeSH

Aptamers, NucleotideBiosensing TechniquesElectrochemistryElectrodesEquipment DesignEquipment Failure AnalysisNanoparticlesNanotechnologyReproducibility of ResultsSensitivity and SpecificityThrombin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19914815

Citation

Ding, Caifeng, et al. "Aptamer Based Electrochemical Assay for the Determination of Thrombin By Using the Amplification of the Nanoparticles." Biosensors & Bioelectronics, vol. 25, no. 6, 2010, pp. 1290-4.
Ding C, Ge Y, Lin JM. Aptamer based electrochemical assay for the determination of thrombin by using the amplification of the nanoparticles. Biosens Bioelectron. 2010;25(6):1290-4.
Ding, C., Ge, Y., & Lin, J. M. (2010). Aptamer based electrochemical assay for the determination of thrombin by using the amplification of the nanoparticles. Biosensors & Bioelectronics, 25(6), 1290-4. https://doi.org/10.1016/j.bios.2009.10.017
Ding C, Ge Y, Lin JM. Aptamer Based Electrochemical Assay for the Determination of Thrombin By Using the Amplification of the Nanoparticles. Biosens Bioelectron. 2010 Feb 15;25(6):1290-4. PubMed PMID: 19914815.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aptamer based electrochemical assay for the determination of thrombin by using the amplification of the nanoparticles. AU - Ding,Caifeng, AU - Ge,Ying, AU - Lin,Jin-Ming, Y1 - 2009/10/21/ PY - 2009/08/10/received PY - 2009/09/27/revised PY - 2009/10/13/accepted PY - 2009/11/17/entrez PY - 2009/11/17/pubmed PY - 2010/4/23/medline SP - 1290 EP - 4 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 25 IS - 6 N2 - A novel electrochemical assay based on the aptamer and the signal of amplification of nanoparticles (NPs) was constructed for the determination of thrombin. Aptamers immobilized on the electrode and Au NPs could be assembled with the target protein to form a sandwich structure in the presence of the latter. Differential pulse voltammetry (DPV) was employed to detect the CdS NPs loaded on the surface of the Au NPs through the linker DNA, which was related to the concentration of the target protein. The assay took advantage of the amplification ability of Au nanoparticles carrying multiplex CdS NPs and the specific affinity of aptamers. Thrombin was detected in this assay in the linear range of 1.0x10(-15) to 1.0x10(-11) M with the detection limit of 5.5x10(-16) M of target protein. In addition, the assay could be used to detection thrombin in real samples with high sensitivity and good selectivity. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19914815/Aptamer_based_electrochemical_assay_for_the_determination_of_thrombin_by_using_the_amplification_of_the_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(09)00562-4 DB - PRIME DP - Unbound Medicine ER -